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Conserved domains on  [gi|1063733387|ref|NP_001332159|]
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magnesium (Mg) transporter 10 [Arabidopsis thaliana]

Protein Classification

CorA family magnesium transporter( domain architecture ID 10191269)

CorA family magnesium transporter, belonging to the CorA metal ion transporter (MIT) family, mediates the influx of magnesium ions, similar to mitochondrial inner membrane magnesium transporter MRS2

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 132-458 1.21e-88

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


:

Pssm-ID: 213357  Cd Length: 323  Bit Score: 272.97  E-value: 1.21e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 132 VVEVKATGAISTRKINRRQLLKSSGLRPRDIRSVDPSLfmTNSVPSLLVREHAILLNLGSLRAIAMRDRVLIFDYNRRGG 211
Cdd:cd12823     1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRLLDPTL--TSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGSSS 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 212 ---RAFVDTLMPRLNPRSMNGGP-----SMPFELEAVESALISRIQRLEQRLMDIEPRVQALLEVLPNRLTADILEELRI 283
Cdd:cd12823    79 alvSAFLEELQRRLASSNGSESEsggedSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLERLLP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 284 SKQRLVELGSRAGALRQMLLDLLEDPHEIRRICIMGRNCTLRRGD--------DDLECTLpsdkliaeeeeeeiemllEN 355
Cdd:cd12823   159 LKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLEssrkeddhEEVEMLL------------------EA 220

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 356 YLQRCESCHGQAERLLDSAKEMEDSIAVNLSSRRLEVSRFELLLQVGTFCVAVGALIAGIFGMNLRSYLEEQASAFWLTT 435
Cdd:cd12823   221 YLQQVDELLNKLEELREYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESPYAFWIVT 300

                         330       340
                  ....*....|....*....|...
gi 1063733387 436 GGIIIGAAVAFFLMYSYLSRRKI 458
Cdd:cd12823   301 GGSVVGSILIFIVVLRYLRRKRL 323
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 132-458 1.21e-88

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 272.97  E-value: 1.21e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 132 VVEVKATGAISTRKINRRQLLKSSGLRPRDIRSVDPSLfmTNSVPSLLVREHAILLNLGSLRAIAMRDRVLIFDYNRRGG 211
Cdd:cd12823     1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRLLDPTL--TSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGSSS 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 212 ---RAFVDTLMPRLNPRSMNGGP-----SMPFELEAVESALISRIQRLEQRLMDIEPRVQALLEVLPNRLTADILEELRI 283
Cdd:cd12823    79 alvSAFLEELQRRLASSNGSESEsggedSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLERLLP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 284 SKQRLVELGSRAGALRQMLLDLLEDPHEIRRICIMGRNCTLRRGD--------DDLECTLpsdkliaeeeeeeiemllEN 355
Cdd:cd12823   159 LKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLEssrkeddhEEVEMLL------------------EA 220

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 356 YLQRCESCHGQAERLLDSAKEMEDSIAVNLSSRRLEVSRFELLLQVGTFCVAVGALIAGIFGMNLRSYLEEQASAFWLTT 435
Cdd:cd12823   221 YLQQVDELLNKLEELREYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESPYAFWIVT 300

                         330       340
                  ....*....|....*....|...
gi 1063733387 436 GGIIIGAAVAFFLMYSYLSRRKI 458
Cdd:cd12823   301 GGSVVGSILIFIVVLRYLRRKRL 323
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 158-450 9.84e-04

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 41.15  E-value: 9.84e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 158 RPRDIRSVDPSLFMTNSVPSLLVREHAILLNLgslrAIAMRDRVLI-FdynRRGGRAFVDTLMPRLNPRsmNGGPSMPFE 236
Cdd:pfam01544  40 RPRVEDYDDYFLLVLPGVNLDDSEEADELVTV----SILLGDNFLItV---RHEPLPALDEVRKRLTLL--GGGPLDPGD 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 237 LeaVESALISRIQRLEQRLMDIEPRVQALLEVLPNRLTADILEELRISKQRLVELGSRAGALRQMLLDLL--EDPHEIRR 314
Cdd:pfam01544 111 L--LYRLLDEIVDRFLELLEKLEDELDELEDELEDETNNELLRELAALRRSLVYLRRSLLPQRDVLNRLLrrEDDPLLDD 188

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 315 icimGRNCTLRRGDDDLEctlpsdkliaeeeeeeiemllenylqrceschgQAERLLDSAKEMEDSIAVNLSSRR-LEVS 393
Cdd:pfam01544 189 ----EQKEYLRDLLDRLE---------------------------------RLLEDLDALRERLRSLQDEYSSLLsERTN 231

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 394 RFELLLQVGTFCVAVGALIAGIFGMNLRSY--LEEQ-ASAFWLTTGGIIIGAAVAFFLMY 450
Cdd:pfam01544 232 RVMKVLTVVTAIFLPLTLITGIYGMNFGGLpeLEWSyGYPFVLILGLMILLAILLLFYFR 291
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 132-458 1.21e-88

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 272.97  E-value: 1.21e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 132 VVEVKATGAISTRKINRRQLLKSSGLRPRDIRSVDPSLfmTNSVPSLLVREHAILLNLGSLRAIAMRDRVLIFDYNRRGG 211
Cdd:cd12823     1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRLLDPTL--TSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGSSS 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 212 ---RAFVDTLMPRLNPRSMNGGP-----SMPFELEAVESALISRIQRLEQRLMDIEPRVQALLEVLPNRLTADILEELRI 283
Cdd:cd12823    79 alvSAFLEELQRRLASSNGSESEsggedSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLERLLP 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 284 SKQRLVELGSRAGALRQMLLDLLEDPHEIRRICIMGRNCTLRRGD--------DDLECTLpsdkliaeeeeeeiemllEN 355
Cdd:cd12823   159 LKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLEssrkeddhEEVEMLL------------------EA 220

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 356 YLQRCESCHGQAERLLDSAKEMEDSIAVNLSSRRLEVSRFELLLQVGTFCVAVGALIAGIFGMNLRSYLEEQASAFWLTT 435
Cdd:cd12823   221 YLQQVDELLNKLEELREYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESPYAFWIVT 300

                         330       340
                  ....*....|....*....|...
gi 1063733387 436 GGIIIGAAVAFFLMYSYLSRRKI 458
Cdd:cd12823   301 GGSVVGSILIFIVVLRYLRRKRL 323
EcCorA_ZntB-like cd12821
Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily ...
 147-459 9.78e-06

Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this family are found in all three kingdoms of life. It is a functionally diverse family, including the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), and the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). It also includes two Saccharomyces cerevisiae members: the inner membrane Mg2+ transporters Mfm1p/Lpe10p, and Mrs2p, and a family of Arabidopsis thaliana members (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213355 [Multi-domain]  Cd Length: 285  Bit Score: 47.31  E-value: 9.78e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 147 NRRQLLKssGLRPRDIRSVDPSLFMtnsvpsLLVREHAILLNLGSLRAIAMRDRVLIFdyNRRGGRAFVDtLMPRLNPRS 226
Cdd:cd12821    26 DLRDILL--GVSFPRFEEVSGSGIY------IQLRKPQKDADTASFHFLITRDRLITV--RLDELPALDE-LRQDLRSGQ 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 227 MNGGPSmpfELEAVESALISRIQRLEQRLMDIEPRVQALLEVLPNRLTADILEELRISKQRLVELGSRAGALRQMLLDLL 306
Cdd:cd12821    95 MKSAKS---AAELLFVLLGAIIKALLTGIDQFEEKLEELEWDLLEGNNAIKLDRILELRRELLRLTNLIEPQQEVLMALQ 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 307 EDPHEIRRiciMGRNCTLRRgdddlectlpsdkliaeeeeeeiemllenYLQRCESCHGQAERLLDSAKEMEDSIAVNLS 386
Cdd:cd12821   172 EAFAELLF---SEDEEELRR-----------------------------TLDRIERLLQLIEEYEQELDTLQDIEEVVSS 219

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1063733387 387 SRRLEVSRFELLLQVgtfcVAVGA-LIAGIFGMNLrSYLEEQ--ASAFWLTTGGIIigaaVAFFLMYSYLsRRKIF 459
Cdd:cd12821   220 HRGNEIMKTLTVVTT----VFLPLtLIGGLWGMNF-KNMPELewKYGYLFVLGLII----LSTAGLYLYL-RRKGW 285
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 407-459 5.14e-04

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 41.72  E-value: 5.14e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1063733387 407 AVGALIAGIFGMNLRSYLEEQASAFWLttggIIIGAAVAFFLMYSYLSRRKIF 459
Cdd:cd12827   241 TIPTIITGFYGMNVPLPFADSPYAFWI----ILLISLLLSVIVALILRRKKYF 289
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 158-450 9.84e-04

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 41.15  E-value: 9.84e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 158 RPRDIRSVDPSLFMTNSVPSLLVREHAILLNLgslrAIAMRDRVLI-FdynRRGGRAFVDTLMPRLNPRsmNGGPSMPFE 236
Cdd:pfam01544  40 RPRVEDYDDYFLLVLPGVNLDDSEEADELVTV----SILLGDNFLItV---RHEPLPALDEVRKRLTLL--GGGPLDPGD 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 237 LeaVESALISRIQRLEQRLMDIEPRVQALLEVLPNRLTADILEELRISKQRLVELGSRAGALRQMLLDLL--EDPHEIRR 314
Cdd:pfam01544 111 L--LYRLLDEIVDRFLELLEKLEDELDELEDELEDETNNELLRELAALRRSLVYLRRSLLPQRDVLNRLLrrEDDPLLDD 188

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 315 icimGRNCTLRRGDDDLEctlpsdkliaeeeeeeiemllenylqrceschgQAERLLDSAKEMEDSIAVNLSSRR-LEVS 393
Cdd:pfam01544 189 ----EQKEYLRDLLDRLE---------------------------------RLLEDLDALRERLRSLQDEYSSLLsERTN 231

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063733387 394 RFELLLQVGTFCVAVGALIAGIFGMNLRSY--LEEQ-ASAFWLTTGGIIIGAAVAFFLMY 450
Cdd:pfam01544 232 RVMKVLTVVTAIFLPLTLITGIYGMNFGGLpeLEWSyGYPFVLILGLMILLAILLLFYFR 291
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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