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Conserved domains on  [gi|296182501|gb|ADG99407|]
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biotin/acetyl-CoA-carboxylase ligase [Segniliparus rotundus DSM 44985]

Protein Classification

biotin--[acetyl-CoA-carboxylase] ligase( domain architecture ID 11417403)

biotin--[acetyl-CoA-carboxylase] ligase catalyzes the formation of biotinyl-5'-AMP from biotin and ATP, and the succeeding biotinylation of the biotin carboxyl carrier protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 29-278 6.86e-74

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


:

Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 226.21  E-value: 6.86e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  29 SVEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAFDRSARaggpELGWLPLAVG 108
Cdd:COG0340    1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPA----RLPLLSLAAG 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 109 LSVGAMLAQYFdsyrsisGVPVpepSLKWPNDVLVGDRKIAGVLVQLSPKADE---AVVGVGLNVDQHEDELP--VPTAT 183
Cdd:COG0340   77 LAVAEALRELT-------GVDV---GLKWPNDILLNGKKLAGILIEASGEGDGidwVVIGIGINVNQPPFDPEelDQPAT 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 184 SLRIEQ-VAAARQQLVPLLLMELDRGLDRWLAQD-PGLADAYRAHCSTLGQDVRVALPGGnEIFGRASSVDDHGRLVVAL 261
Cdd:COG0340  147 SLKEETgKEVDREELLAALLEELEELYDRFLEEGfAPILEEWRARLATLGRRVRVETGGE-TLEGIAVGIDEDGALLLET 225

                        250
                 ....*....|....*..
gi 296182501 262 ADGTARVVDAGDVqHVR 278
Cdd:COG0340  226 ADGEIRAVAAGEV-SLR 241
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 29-278 6.86e-74

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 226.21  E-value: 6.86e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  29 SVEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAFDRSARaggpELGWLPLAVG 108
Cdd:COG0340    1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPA----RLPLLSLAAG 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 109 LSVGAMLAQYFdsyrsisGVPVpepSLKWPNDVLVGDRKIAGVLVQLSPKADE---AVVGVGLNVDQHEDELP--VPTAT 183
Cdd:COG0340   77 LAVAEALRELT-------GVDV---GLKWPNDILLNGKKLAGILIEASGEGDGidwVVIGIGINVNQPPFDPEelDQPAT 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 184 SLRIEQ-VAAARQQLVPLLLMELDRGLDRWLAQD-PGLADAYRAHCSTLGQDVRVALPGGnEIFGRASSVDDHGRLVVAL 261
Cdd:COG0340  147 SLKEETgKEVDREELLAALLEELEELYDRFLEEGfAPILEEWRARLATLGRRVRVETGGE-TLEGIAVGIDEDGALLLET 225

                        250
                 ....*....|....*..
gi 296182501 262 ADGTARVVDAGDVqHVR 278
Cdd:COG0340  226 ADGEIRAVAAGEV-SLR 241
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 30-212 3.90e-47

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 155.50  E-value: 3.90e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  30 VEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAFDRSARaggpELGWLPLAVGL 109
Cdd:cd16442    2 LIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLRPDVPPA----EAPLLTLLAAV 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 110 SVGAMLAQYFDsyrsisgvpvPEPSLKWPNDVLVGDRKIAGVLVQLSPKADE---AVVGVGLNVDQHEDELPVPTATSLR 186
Cdd:cd16442   78 AVAEALEKLGG----------IPVQIKWPNDILVNGKKLAGILTEASAEGEGvaaVVIGIGINVNNTPPPEPLPDTSLAT 147

                        170       180
                 ....*....|....*....|....*.
gi 296182501 187 IEQVAAARQQLVPLLLMELDRGLDRW 212
Cdd:cd16442  148 SLGKEVDRNELLEELLAALENRLELF 173
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
10-264 2.40e-36

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 131.83  E-value: 2.40e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  10 LGAAQLRQYLSNSPplgwesVEVIEETGSTNADLLERAARGPiARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAF 89
Cdd:PRK11886  66 LDPERISSQLPPGR------VTVLPVIDSTNQYLLDRIAELK-SGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWRL 138

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  90 DrsaraGGP-ELGWLPLAVGLSVgamlaqyfdsYRSISGVPVPEPSLKWPNDVLVGDRKIAGVLVQLSPKADEA---VVG 165
Cdd:PRK11886 139 N-----QGPaQAMGLSLVVGIAI----------AEALRRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAahvVIG 203

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 166 VGLNV---DQHEDELPVPTaTSLRIEQVAAARQQLVPLLLMELDRGLDRWLAQdpGLAD---AYRAHCSTLGQDVRVaLP 239
Cdd:PRK11886 204 IGINVampDFPEELIDQPW-SDLQEAGPTIDRNQLAAELIKQLRAALELFEQE--GLAPfleRWKKLDLFLGREVKL-II 279

                        250       260
                 ....*....|....*....|....*
gi 296182501 240 GGNEIFGRASSVDDHGRLVVALADG 264
Cdd:PRK11886 280 GDKEISGIARGIDEQGALLLEDDGV 304
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 29-277 1.54e-31

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 117.12  E-value: 1.54e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501   29 SVEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRtqiALSFGVAFDrsaraggPELGWLP---- 104
Cdd:TIGR00121   1 EVIVLDVIDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEG---GLYFSLILR-------PDLPKSPapgl 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  105 -LAVGLSVGAMLAQYfdsyrsisGVPVpepSLKWPNDVLVGDRKIAGVLVQLSPKADE---AVVGVGLNVD-QHEDELPV 179
Cdd:TIGR00121  71 tLVAGIAIAEVLKEL--------GDQV---QVKWPNDILLKDKKLGGILTELTGKENRadyVVIGIGINVQnRKPAESLR 139

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  180 PTATSLRIE-QVAAARQQLVPLLLMELDRGLdRWLAQ---DPGLADaYRAHCSTLGQDVRVALPGGnEIFGRASSVDDHG 255
Cdd:TIGR00121 140 EQAISLSEEaGIDLDRGELIEGFLRNFEENL-EWFEQegiDEILSK-WEKLSAHIGREVSLTTGNG-EIEGIARGIDKDG 216

                         250       260
                  ....*....|....*....|..
gi 296182501  256 RLVVaLADGTARVVDAGDVQHV 277
Cdd:TIGR00121 217 ALLL-EDGGGIKKIISGEISLR 237
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 32-169 6.44e-14

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 67.08  E-value: 6.44e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501   32 VIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTqiaLSFGVAFdrSARAGGPELGWLPLAVGLSV 111
Cdd:pfam03099   1 LGERIKSTNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKGC---LTYSLLL--SKEHPNVDPSVLEFYVLELV 75

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  112 GAMLAQYFDSYRSISGVPVPepsLKWPNDVLVGDRKIAGVLV--QLSPKADEAVVGVGLN 169
Cdd:pfam03099  76 LAVLEALGLYKPGISGIPCF---VKWPNDLYVNGRKLAGILQrsTRGGTLHHGVIGLGVN 132
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 29-278 6.86e-74

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 226.21  E-value: 6.86e-74
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  29 SVEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAFDRSARaggpELGWLPLAVG 108
Cdd:COG0340    1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPA----RLPLLSLAAG 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 109 LSVGAMLAQYFdsyrsisGVPVpepSLKWPNDVLVGDRKIAGVLVQLSPKADE---AVVGVGLNVDQHEDELP--VPTAT 183
Cdd:COG0340   77 LAVAEALRELT-------GVDV---GLKWPNDILLNGKKLAGILIEASGEGDGidwVVIGIGINVNQPPFDPEelDQPAT 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 184 SLRIEQ-VAAARQQLVPLLLMELDRGLDRWLAQD-PGLADAYRAHCSTLGQDVRVALPGGnEIFGRASSVDDHGRLVVAL 261
Cdd:COG0340  147 SLKEETgKEVDREELLAALLEELEELYDRFLEEGfAPILEEWRARLATLGRRVRVETGGE-TLEGIAVGIDEDGALLLET 225

                        250
                 ....*....|....*..
gi 296182501 262 ADGTARVVDAGDVqHVR 278
Cdd:COG0340  226 ADGEIRAVAAGEV-SLR 241
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 30-212 3.90e-47

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 155.50  E-value: 3.90e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  30 VEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAFDRSARaggpELGWLPLAVGL 109
Cdd:cd16442    2 LIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLRPDVPPA----EAPLLTLLAAV 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 110 SVGAMLAQYFDsyrsisgvpvPEPSLKWPNDVLVGDRKIAGVLVQLSPKADE---AVVGVGLNVDQHEDELPVPTATSLR 186
Cdd:cd16442   78 AVAEALEKLGG----------IPVQIKWPNDILVNGKKLAGILTEASAEGEGvaaVVIGIGINVNNTPPPEPLPDTSLAT 147

                        170       180
                 ....*....|....*....|....*.
gi 296182501 187 IEQVAAARQQLVPLLLMELDRGLDRW 212
Cdd:cd16442  148 SLGKEVDRNELLEELLAALENRLELF 173
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
10-264 2.40e-36

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 131.83  E-value: 2.40e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  10 LGAAQLRQYLSNSPplgwesVEVIEETGSTNADLLERAARGPiARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAF 89
Cdd:PRK11886  66 LDPERISSQLPPGR------VTVLPVIDSTNQYLLDRIAELK-SGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWRL 138

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  90 DrsaraGGP-ELGWLPLAVGLSVgamlaqyfdsYRSISGVPVPEPSLKWPNDVLVGDRKIAGVLVQLSPKADEA---VVG 165
Cdd:PRK11886 139 N-----QGPaQAMGLSLVVGIAI----------AEALRRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAahvVIG 203

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 166 VGLNV---DQHEDELPVPTaTSLRIEQVAAARQQLVPLLLMELDRGLDRWLAQdpGLAD---AYRAHCSTLGQDVRVaLP 239
Cdd:PRK11886 204 IGINVampDFPEELIDQPW-SDLQEAGPTIDRNQLAAELIKQLRAALELFEQE--GLAPfleRWKKLDLFLGREVKL-II 279

                        250       260
                 ....*....|....*....|....*
gi 296182501 240 GGNEIFGRASSVDDHGRLVVALADG 264
Cdd:PRK11886 280 GDKEISGIARGIDEQGALLLEDDGV 304
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 29-277 1.54e-31

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 117.12  E-value: 1.54e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501   29 SVEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRtqiALSFGVAFDrsaraggPELGWLP---- 104
Cdd:TIGR00121   1 EVIVLDVIDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEG---GLYFSLILR-------PDLPKSPapgl 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  105 -LAVGLSVGAMLAQYfdsyrsisGVPVpepSLKWPNDVLVGDRKIAGVLVQLSPKADE---AVVGVGLNVD-QHEDELPV 179
Cdd:TIGR00121  71 tLVAGIAIAEVLKEL--------GDQV---QVKWPNDILLKDKKLGGILTELTGKENRadyVVIGIGINVQnRKPAESLR 139

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  180 PTATSLRIE-QVAAARQQLVPLLLMELDRGLdRWLAQ---DPGLADaYRAHCSTLGQDVRVALPGGnEIFGRASSVDDHG 255
Cdd:TIGR00121 140 EQAISLSEEaGIDLDRGELIEGFLRNFEENL-EWFEQegiDEILSK-WEKLSAHIGREVSLTTGNG-EIEGIARGIDKDG 216

                         250       260
                  ....*....|....*....|..
gi 296182501  256 RLVVaLADGTARVVDAGDVQHV 277
Cdd:TIGR00121 217 ALLL-EDGGGIKKIISGEISLR 237
PRK08330 PRK08330
biotin--protein ligase; Provisional
 56-274 2.97e-29

biotin--protein ligase; Provisional


Pssm-ID: 169384 [Multi-domain]  Cd Length: 236  Bit Score: 110.99  E-value: 2.97e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  56 VLIADHQTQGKGRLGRRWEAPPRtqiALSFGVAFdrSARAGGPELGWLPLAVGLSVGAMLAQYfdsyrSISGvpvpepSL 135
Cdd:PRK08330  30 VIVADRQTAGHGRKGRAWASPEG---GLWMSVIL--KPKVSPEHLPKLVFLGALAVVDTLREF-----GIEG------KI 93

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 136 KWPNDVLVGDRKIAGVLVQLspKADEAVVGVGLNVDQHEDELPVPTATSLRIEQVAAarqqlVPLL--LMELDRGLDRW- 212
Cdd:PRK08330  94 KWPNDVLVNYKKIAGVLVEG--KGDFVVLGIGLNVNNEIPDELRETATSMKEVLGRE-----VPLIevFKRLVENLDRWy 166

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 296182501 213 ---LAQDPGLADAYRAHCSTLGQDVRVALPGGNEIFGRASSVDDHGRLVVALADGTARVVDAGDV 274
Cdd:PRK08330 167 klfLEGPGEILEEVKGRSMILGKRVKIIGDGEILVEGIAEDIDEFGALILRLDDGTVKKVLYGDV 231
PRK06955 PRK06955
biotin--[acetyl-CoA-carboxylase] ligase;
29-274 3.19e-25

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 235896 [Multi-domain]  Cd Length: 300  Bit Score: 101.78  E-value: 3.19e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  29 SVEVIEETGSTNADL------LERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTQIALSFGVAFDRSARAggpeLGW 102
Cdd:PRK06955  34 PLEIVEETGSTNADLmarlkaLPRSADALPAPIVRVAYEQTAGRGRQGRPWFAQPGNALLFSVACVLPRPVAA----LAG 109

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 103 LPLAVGLSVGAMLAQYFDSYRSISGvpvpepsLKWPNDVLVGDRKIAGVLVQLSPKADEA---VVGVGLNVDQHEDELPV 179
Cdd:PRK06955 110 LSLAVGVALAEALAALPAALGQRIA-------LKWPNDLLIAGRKLAGILIETVWATPDAtavVIGIGLNVRRADAVAAE 182

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 180 PTATSLRI-----------EQVAAARQQLVPLLLMELDR---GLDRWLAQdpGLA---------DAYRahcstlGQDVRV 236
Cdd:PRK06955 183 VDALRAREaalarglppvaLAAACAGANLTDTLAAALNAlapALQAFGAD--GLApfaarwhalHAYA------GREVVL 254

                        250       260       270
                 ....*....|....*....|....*....|....*...
gi 296182501 237 ALPGGNEIFGRASSVDDHGRLVVALADGTARVVdAGDV 274
Cdd:PRK06955 255 LEDGAELARGVAHGIDETGQLLLDTPAGRQAIA-AGDV 291
BirA COG1654
Biotin operon repressor [Transcription];
4-274 2.24e-16

Biotin operon repressor [Transcription];


Pssm-ID: 441260 [Multi-domain]  Cd Length: 324  Bit Score: 77.72  E-value: 2.24e-16
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501   4 KDPREPLGAAQLRQYLSnSPPLGWEsVEVIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTQIAL 83
Cdd:COG1654   60 AEPPDLLDPEEIRAGLS-TKRLGRE-ILYVISSTSTNLLALELAAQGGDAGTVVAAEQQRGGRGRRRRSWSSPGGGGLLY 137

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  84 SFGVAFDRSARAggpelgwLPLAVGLSVGAMLAQyfdsyrsISGVPVPEPSLKWPNDVLVGDRKIAGVLVQLSPKADEAV 163
Cdd:COG1654  138 SLLLRPPIAPAL-------LSLLLLAAAVAVAAA-------LAEGGGLVKWKKWPNDLLKKGKKILGILEEEGGDADGVV 203

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 164 VGVGLNV-------DQHEDELPVPTATSLRIEQVAAARQQLVPLLLMELDRGLDRWLAQDPGLADAYRAHCSTLGQDVRV 236
Cdd:COG1654  204 IVVGGGGnnnnsnpEEEPQELAELATSLLLILRLRLLRLLLLLLLLLLELLELLGFLEFFFLWERLDWELLRVLKLVVVV 283

                        250       260       270
                 ....*....|....*....|....*....|....*...
gi 296182501 237 ALPGGNEIFGRASSVDDHGRLVVALADGTARVVDAGDV 274
Cdd:COG1654  284 VEIGGGGGGGGALGGGLLGLLLLGGGGGGGEGSLSAVV 321
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 32-169 6.44e-14

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 67.08  E-value: 6.44e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501   32 VIEETGSTNADLLERAARGPIARRVLIADHQTQGKGRLGRRWEAPPRTqiaLSFGVAFdrSARAGGPELGWLPLAVGLSV 111
Cdd:pfam03099   1 LGERIKSTNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKGC---LTYSLLL--SKEHPNVDPSVLEFYVLELV 75

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  112 GAMLAQYFDSYRSISGVPVPepsLKWPNDVLVGDRKIAGVLV--QLSPKADEAVVGVGLN 169
Cdd:pfam03099  76 LAVLEALGLYKPGISGIPCF---VKWPNDLYVNGRKLAGILQrsTRGGTLHHGVIGLGVN 132
PRK13325 PRK13325
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;
35-279 2.27e-11

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;


Pssm-ID: 183976 [Multi-domain]  Cd Length: 592  Bit Score: 63.96  E-value: 2.27e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  35 ETGSTNADLLERAARGP-IARRVLIADH-QTQGKGRLGRRWEAPPRTQIALSFGVAFDRSARaggpELGWLPLAVGLSVg 112
Cdd:PRK13325  89 ECASSNDEILELARIAPdKAHKTICVTHlQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQY----ELGSLSPVAAVAC- 163

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 113 amlaqyfdsyRSISGVPVPEPSLKWPNDVLVGDRKIAGVLVQLSPKADE--AVVGVGLNVDQHEDelpVPTATSLR-IEQ 189
Cdd:PRK13325 164 ----------RRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKtvAVVGIGINFVLPKE---VENAASVQsLFQ 230

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 190 VAAARQQ-----LVPLLLMELDRGLDRWlaQDPGLA---DAYRAHCSTLGQDVRVALPGGNEIFGRASSVDDHGRLVVAL 261
Cdd:PRK13325 231 TASRRGNadaavLLETLLAELDAVLLQY--ARDGFApfvAEYQAANRDHGKAVLLLRDGETVFEGTVKGVDGQGVLHLET 308

                        250
                 ....*....|....*...
gi 296182501 262 ADGTARVVdAGDVQhVRP 279
Cdd:PRK13325 309 AEGKQTVV-SGEIS-LRS 324
PRK05935 PRK05935
biotin--protein ligase; Provisional
 33-198 6.66e-10

biotin--protein ligase; Provisional


Pssm-ID: 235649 [Multi-domain]  Cd Length: 190  Bit Score: 57.13  E-value: 6.66e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  33 IEETGSTNAdlleRAARG-----PIARRVLIADHQTQGKGRLGRRWEAPPRtQIALSF-------GVAFDRSARAGGPEL 100
Cdd:PRK05935   8 IAETPSTNT----TAKEGmhlwdPYALTVISTREQTAGKGKFGKSWHSSDQ-DLLASFcffitvlNIDVSLLFRLGTEAV 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 101 gwlpLAVGLSVGamlaqyfdsyrsisgvpVPEPSLKWPNDVLVGDRKIAGVLVQLSP--KADEAVVGVGLNVDQHEDELp 178
Cdd:PRK05935  83 ----MRLGEDLG-----------------ITEAVIKWPNDVLVHGEKLCGVLCETIPvkGGLGVILGIGVNGNTTKDEL- 140

                        170       180
                 ....*....|....*....|
gi 296182501 179 vptatsLRIEQVAAARQQLV 198
Cdd:PRK05935 141 ------LGIDQPATSLQELL 154
PRK08477 PRK08477
biotin--[acetyl-CoA-carboxylase] ligase;
30-169 1.70e-09

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 236273 [Multi-domain]  Cd Length: 211  Bit Score: 56.50  E-value: 1.70e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  30 VEVIEETGSTNADLLERAARGPIARRVLI-ADHQTQGKGRLGRRWEAPPRtqiALSFGVAFDRSARAGGpelgwLPLA-V 107
Cdd:PRK08477   3 IRVFESLDSTQTYLIEKIKNGELKAPFAIvAKEQTAGIGSRGNSWEGKKG---NLFFSFALKESDLPKD-----LPLQsS 74

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 296182501 108 GLSVGAMLAQYFDSYRSisgvpvpEPSLKWPNDVLVGDRKIAGVLVQLSpkADEAVVGVGLN 169
Cdd:PRK08477  75 SIYFGFLLKEVLKELGS-------KVWLKWPNDLYLDDKKIGGVITNKI--KNFIVCGIGLN 127
PTZ00276 PTZ00276
biotin/lipoate protein ligase; Provisional
 20-271 2.41e-08

biotin/lipoate protein ligase; Provisional


Pssm-ID: 140302 [Multi-domain]  Cd Length: 245  Bit Score: 53.72  E-value: 2.41e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  20 SNSPPlgweSVEVIEETGST-NADLLERAARGPIARRVLiADHQTQGKGRLGRRWEAPpRTQIALSFGVafdrsaraggP 98
Cdd:PTZ00276   3 ADVPP----NIHFVGEVTSTmDVARTMLAAAGGKPFAVL-AESQTAGRGTGGRTWTSP-KGNMYFTLCI----------P 66

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501  99 ELGW-------LPLAVGLSVGAMLAQYfdsyrsISGVPVpepSLKWPNDVLVGDRKIAGVLVQlsPKADEAVVGVGLNVd 171
Cdd:PTZ00276  67 QKGVppelvpvLPLITGLACRAAIMEV------LHGAAV---HTKWPNDIIYAGKKIGGSLIE--SEGEYLIIGIGMNI- 134

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 296182501 172 qhEDELPVPTA--TSLRIEQVAAA--RQQLVPLLLMEL----------DRGLDRwlaqdPGLADAY-RAHCSTLGQDVRv 236
Cdd:PTZ00276 135 --EVAPPVTDAgrESTMVNEIAEDlgVKSVTPQDLAEAvwkhffdicsDPELTR-----EILIESFdAAMDKSLKLHKR- 206

                        250       260       270
                 ....*....|....*....|....*....|....*.
gi 296182501 237 aLPGGNEIFG-RASSVDDHGRLVVALADGTARVVDA 271
Cdd:PTZ00276 207 -TPTGRDPEElTALSLNEWGHLIVRRPDGTEEDLMA 241
BPL_C pfam02237
Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It ...
 229-276 8.44e-08

Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It is found to the C terminus of the biotin protein ligase catalytic domain pfam01317.


Pssm-ID: 426672 [Multi-domain]  Cd Length: 48  Bit Score: 47.84  E-value: 8.44e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 296182501  229 TLGQDVRVALPGGnEIFGRASSVDDHGRLVVALADGTARVVDAGDVQH 276
Cdd:pfam02237   1 TLGREVRVLLGDG-IVEGIAVGIDDDGALLLETDDGTIRDINSGEVSL 47
PTZ00275 PTZ00275
biotin-acetyl-CoA-carboxylase ligase; Provisional
 135-173 9.97e-03

biotin-acetyl-CoA-carboxylase ligase; Provisional


Pssm-ID: 185536 [Multi-domain]  Cd Length: 285  Bit Score: 36.72  E-value: 9.97e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 296182501 135 LKWPNDVLVGDRKIAGVLVQLSPKADEA---------VVGVGLNV---DQH 173
Cdd:PTZ00275 122 IKWINDVLVNYKKIAGCLVHLYYLDDFPnlnsryvcvMVGIGINVtleDKH 172
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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