4G24,2QIP,4CHG,3Q8K,5MQ8


Conserved Protein Domain Family
PIN_SF

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cd09852: PIN_SF 
Click on image for an interactive view with Cn3D
PIN (PilT N terminus) domain: Superfamily
The PIN (PilT N terminus) domain belongs to a large nuclease superfamily, and were originally named for their sequence similarity to the N-terminal domain of an annotated pili biogenesis protein, PilT, a domain fusion between a PIN-domain and a PilT ATPase domain. The structural properties of the PIN domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions; in some members, additional metal coordinating residues can be found while some others lack several of these key catalytic residues. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons. The PIN domain superfamily includes: the FEN-like PIN domain family such as the PIN domains of Flap endonuclease-1 (FEN1), exonuclease-1 (EXO1), Mkt1, Gap Endonuclease 1 (GEN1), and Xeroderma pigmentosum complementation group G (XPG) nuclease, 5'-3' exonucleases of DNA polymerase I and bacteriophage T4- and T5-5' nucleases; the VapC-like PIN domain family which includes toxins of prokaryotic toxin/antitoxin operons FitAB and VapBC, as well as eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1; the LabA-like PIN domain family which includes the PIN domains of Synechococcus elongatus LabA (low-amplitude and bright); the PRORP-Zc3h12a-like PIN domain family which includes the PIN domains of RNase P (PRORP), ribonuclease Zc3h12a; and Bacillus subtilis YacP/Rae1-like PIN domains. It also includes the Mut7-C PIN domain family, which is not represented here as it is a shortened version of the PIN fold and lacks a core strand and helix (H3 and S3). The Mut7-C PIN domain family includes the C-terminus of Caenorhabditis elegans exonuclease Mut-7.
Statistics
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PSSM-Id: 350203
Aligned: 5 rows
Threshold Bit Score: 73.4288
Created: 17-Mar-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1: active site [active site], 4 residue positions
Conserved feature residue pattern:[DENQ] [DENQ] [DENQ] [DENQ]Click to see conserved feature residue pattern help
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1       #                                                                             
4G24_A    326 VIDGANMGLvnqrsfs------------ffqlnnTVQrcqqis---pskrlPLVILhksrvngg---------------- 374 thale cress
2QIP_A     15 LVDVQNVYYtcrea---------------yrsnfDYNqfwyvatqekevvsAKAYAias--------------------- 58  Vibrio parahaemoly...
4CHG_A      3 IVDTSVWIAylstse--------------slasrWLAdria------adstVIVPEvvxxellig--------------- 47  Mycobacterium tube...
3Q8K_A     31 AIDASMSIYqfliavrqggdvlqneegettshlmGMFyrtirm--mengikPVYVFdgkppqlksgelakrserraeaek 108 human
5MQ8_A      5 LVDGYNMIGawpqlkdlkan---sfeeardvliqKMAeyqsy-----tgnrVIVVFdahlv------------------- 57  Bacillus subtilis ...
Feature 1                                                                                #    
4G24_A    375 ---------------------patypknRALlekw----knagALYAtppg---------------------sndDWYWL 408 thale cress
2QIP_A     59 --------------------------ndPKQrqfhhilrgvgfEVXLkpyiqrrd-------------gsakgdwDVGIT 99  Vibrio parahaemoly...
4CHG_A     48 -------------------ktdedtaalRRRllq-------rfAIEPlapvrdaedaaaihrrcrrggdtvrsliDCQVA 101 Mycobacterium tube...
3Q8K_A    109 qlqqaqaagaeqevekftkrlvkvtkqhNDEckhll--slmgiPYLDaps-----------------------eaEASCA 163 human
5MQ8_A     58 -----------------------kglekKQTnh--------rvEVIFtken---------------------etaDERIE 85  Bacillus subtilis ...
Feature 1                       #   #                     
4G24_A    409 YAAvsc-----kcLLVTNDemrDHLfqllgnsffprwkekHQVR 447 thale cress
2QIP_A    100 LDAieiapdvdrvILVSGDg--DFSllveriq-qrynkkvTVYG 140 Vibrio parahaemolyticus RIMD 2210633
4CHG_A    102 AXAlri-----gvAVAHRDr--DYEairt-------hcglRTEP 131 Mycobacterium tuberculosis H37Rv
3Q8K_A    164 ALVkag----kvyAAATEDm--DCLtfg----------spVLMR 191 human
5MQ8_A     86 KLAqalnniatqiHVATSDy-tEQWaifg--------qgaLRKS 120 Bacillus subtilis subsp. subtilis str. 168

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