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cd01663: Cyt_c_Oxidase_I 
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Cytochrome C oxidase subunit I. Cytochrome c oxidase (CcO), the terminal oxidase in the respiratory chains of eukaryotes and most bacteria, is a multi-chain transmembrane protein located in the inner membrane of mitochondria and the cell membrane of prokaryotes. It catalyzes the reduction of O2 and simultaneously pumps protons across the membrane. The number of subunits varies from three to five in bacteria and up to 13 in mammalian mitochondria. Only subunits I and II are essential for function, but subunit III, which is also conserved, may play a role in assembly or oxygen delivery to the active site. Subunits I, II, and III of mammalian CcO are encoded within the mitochondrial genome and the remaining 10 subunits are encoded within the nuclear genome. Subunit I contains a heme-copper binuclear center (the active site where O2 is reduced to water) formed by a high-spin heme (heme a3) and a copper ion (CuB). It also contains a low-spin heme (heme a), believed to participate in the transfer of electrons to the binuclear center. For every reduction of an O2 molecule, eight protons are taken from the inside aqueous compartment and four electrons are taken from cytochrome c on the opposite side of the membrane. The four electrons and four of the protons are used in the reduction of O2; the four remaining protons are pumped across the membrane. This charge separation of four charges contributes to the electrochemical gradient used for ATP synthesis. Two proton channels, the D-pathway and K-pathway, leading to the binuclear center have been identified in subunit I. A well-defined pathway for the transfer of pumped protons beyond the binuclear center has not been identified. Electrons are transferred from cytochrome c (the electron donor) to heme a via the CuA binuclear site in subunit II, and directly from heme a to the binuclear center.
Statistics
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PSSM-Id: 238833
View PSSM: cd01663
Aligned: 90 rows
Threshold Bit Score: 647.619
Threshold Setting Gi: 12248350
Created: 10-Apr-2003
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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Display:
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Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Binuclear center (heme a3/CuB) [ion binding site]
Evidence:
  • Comment:Heme a3 and CuB form the binuclear center, where O2 is reduced to water.
  • Structure:1AR1_A: H411 is the axial ligand of heme a3; CuB ligands are H276, H325, and H326
    View structure with Cn3D
  • Comment:Electron transfer is believed to proceed from CuA in subunit II via heme a to the binuclear center.
  • Citation:PMID 9443344

cd01663 is part of a hierarchy of related CD models.
Use the graphical representation to navigate this hierarchy.
cd01663 is a member of the superfamily cl00275.
cd00919:Heme_Cu_Oxidase_Icd01660:ba3-like_Oxidase_Icd01661:cbb3_Oxidase_Icd01662:Ubiquinol_Oxidase_Icd01663:Cyt_c_Oxidase_I1M56 A1AR1 A6110178846203809134762262301422352698853425201873410190917545082520076653024901953804942542988942306182621110292417226721044421711697221689541771104611465927491471711146660111690311169030116902754407841069491352132114665596647494391564850234085627362294160366127187942363029227385245857858876297675249751827509822653443312188942632185553382412OCC N5145092721362442116971117002116997621845115198009831218885200043058352354635843249146904122483311224835012248327555833055158835835446131957464914726362161268181574523432877272124571512859352220942123285895183006451830050126419521264196812642024116963491473704914742227262533670777991338326227228455571577857335691403635001M56 A1AR1 A6110178846203809134762262301422352698853425201873410190917545082520076653024901953804942542988942306182621110292417226721044421711697221689541771104611465927491471711146660111690311169030116902754407841069491352132114665596647494391564850234085627362294160366127187942363029227385245857858876297675249751827509822653443312188942632185553382412OCC N514509272136244211697111700211699762184511519800983121888520004305835235463584324914690412248331122483501224832755583305515883583544613195746491472636216126818157452343287727212457151285935222094212328589518300645183005012641952126419681264202411696349147370491474222726253367077799133832622722845557157785733569140363500
cd01663 Sequence Cluster
cd01663 Sequence Cluster
Sub-family Hierarchy
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 cd01663 Branch
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CD Hierarchy
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2OCC_N       10 TNHKDIGTLYLLFGAWAGMVGTALSLLIRAELGQPgtl-----------------lgDDQIYNVVVTAHAFVMIFFMVMP 72
1AR1_A       26 TNHKDIGILYLFTAGIVGLISVCFTVYMRMELQHPgvqymclegarliadasaectpNGHLWNVMITYHGVLMMFFVVIP 105
gi 67077799   9 TNHKDIGTLYFTLAAWAGMMGTSLSVLIRIELSQPgsf-----------------igSDQTYNALVTAHAFIMIFFMVMP 71
gi 62184511  11 TNHKDIGTLYFLFGVGPGMPGSVTSLLIRMKLIQPges-----------------sgGDSLYNMFITYHALTMIFFMVMP 73
gi 51980098   7 TNHKDIGTLYFLVGIWTGLVATSMSLLIREELGQPgtl-----------------lgDDQIYNCLITAHGLLMMFFVVLP 69
gi 13383262   8 TNHKDIGSLYLIFGIWSGLIGTAFSFLIRSELSQPgsi-----------------lnDSQLYNSIITAHGLIMIFFFVMP 70
gi 27228455  18 TNHKDIGTLYFIFGTWSGIIGTSLSVIIRMELSQGggv-----------------inNGQIYNVVVTSHAFVMIFFFVMP 80
gi 57157785   8 TNHKDIGTLYFLFGMWAGMIGTGMSVLIRIELGKAgtv----------------wadDGQVYNVMVTAHAFVMIFFFVMP 71
gi 7335691   12 TNHKDIGTLYFLFGVWAGFVGTGMSVFIRMELSQVgqv-----------------igDGQLYNVVVTAHAFVMIFFFVMP 74
gi 40363500  12 TNHKDIGTLYFIFGAWAAMMGTALSAIIRIELGSPgty-----------------inDGHLYNAIVTAHAFMMIFFAVMP 74
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2OCC_N       73 iMIGGFGNWLVPLMIGAPDMAFPRMNNMSFWLLPPSFLLLLASSMve-----aGAGTGWTVYPPLAGNlahaGASVDLTI 147
1AR1_A      106 aLFGGFGNYFMPLHIGAPDMAFPRLNNLSYWMYVCGVALGVASLLapggndqmGSGVGWVLYPPLSTTe--aGYSMDLAI 183
gi 67077799  72 aMIGGFGNWLIPMMIGSPDMAFPRLNNMSFWLLIPSLFFLSMSMFsg-----sGAGTGWTVYPPLSGNlahsGFSVDLTI 146
gi 62184511  74 iMIGGFGNWLVPLMLGAPDMAFPRLNNMSFWLLPPSFLLLMSTAWfn-----sGVGTGWTIYPPLSGNlahaGPSMDLAI 148
gi 51980098  70 iLMGGFGNWLVPLMLGAPDMAFPRINNLGFWLIPPAVILLVMSAFie-----kGAGTGWTVYPPLASNiahaGPCIDLAI 144
gi 13383262  71 vLIGGFGNWLIPLYLASPDMAFPRLNNMSFWLLPPAFFLLLGSFIve-----gGVGGGWTIYPPLSSNiahsGPSADMAI 145
gi 27228455  81 iMVGGFGNWLIPLMVGAPDMAFPRMNNMSFWLLPPSFFLLLKSGFsg----saGVGTGWTVYPPLSANighsNTGVDMAI 156
gi 57157785  72 vTLGGFGNWLIPLMVGAPDMAFPRMNNLSFWLLPPAFFLLLLSSFvg-----gGAGTGWTVYPPLSGNlahaGPSVDCAI 146
gi 7335691   75 vMIGGFGNWLLPLMLGCPDMAFPRLNNMSFWLLPPALFMLLLSSVie-----sGVGTGWTVYPPLSGNlahsGPALDCAI 149
gi 40363500  75 iLIGGFGNWLVPLLLGSPDMAFPRLNNLSFWLLPPSLLLLLVSSLvd-----kGVGAGWTVYPPLSGLvgqnGPSMDLAI 149
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2OCC_N      148 FSLHLAGVSSILGAINFITTIINMKPpamsqyqtPLFVWSVMITAVLLLLSLPVLAAGITMLLTDRNLNTTFFDpaGGGD 227
1AR1_A      184 FAVHVSGASSILGAINIITTFLNMRApgmtlfkvPLFAWSVFITAWLILLSLPVLAGAITMLLMDRNFGTQFFDpaGGGD 263
gi 67077799 147 FSLHLAGISSILGAINFITTILSATAntw--ermPLFVWSVLVTTILLLLSLPVLAGAITMLLTDRNFNTSFFDpsGGGD 224
gi 62184511 149 FALHLAGASSILGAINFITTCINMSPhhmspynwPLFVWSVFLTAILLLLSLPVLAAAITMLLTDRNLNTTFFDpnGGGD 228
gi 51980098 145 FALHLSGISSILASINFITTVMNMRYkglrlervPLFVWSVKLTAVLLLLSIPVLAGGLTMLLTDRNLNTSFFDpaGGGD 224
gi 13383262 146 FSLHLAGVSSILGSINFIITMVNAKInlaw-gqlPLFLWAVMVTAYMLVLSLPVLAGGLTMLLTDRNFNTTFFDpgGGGD 224
gi 27228455 157 FSLHLAGVSSILGSVNFLVTLFNMKNkgksmsnlSLFCWSLIVTTILLVLSLPVLAAAITMLLFDRNFNTTFFDpnGGGD 236
gi 57157785 147 FSLHLAGVSSILGSINFLVTMMNMMSkgqtygnlSLFCWSIVVTTILLILSLPVLAAAITMLLFDRNFNTSFFDpaGGGD 226
gi 7335691  150 FSLHLAGVSSILGSLNFMTTMFNMGLkggglfmmPLFCWSVLITTLLLLLSLPVLAAAITMLLFGRNLNTSFFDpaGGGD 229
gi 40363500 150 FSLHLAGASSLAGAINFLVTIFQARSpgmtleriPLYPWAVAVTALLLVLALPVLAGAITMLLTDRNFNTSFFSpdGGGD 229
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   #                                                 ##                
2OCC_N      228 PILYQHLFWFFGHPEVYILILPGFGMISHIVTYYsgkKEPFGYMGMVWAMMSIGFLGFIVWAHHMFTVGMDVDTRAYFTS 307
1AR1_A      264 PVLYQHILWFFGHPEVYIIILPGFGIISHVISTFa-kKPIFGYLPMVLAMAAIGILGFVVWAHHMYTAGMSLTQQAYFML 342
gi 67077799 225 PILFQHLFWFFGHPEVYILILPGFGLISHILSISsgkKEPVGSMAMVHAILSIGILGFIVWGHHMFTVGMDVDSRAYFSA 304
gi 62184511 229 PILFQHLFWFFGHPEVYILILPGFGMISHIVTHHsskKEPFGYMSMVWAMLAITVLGFIVWAHHMFTVGLDVDTRAYFSA 308
gi 51980098 225 PVLYQHLFWFFGHPEVYILVLPGFGLISHLVAHHsakLEPFGSLGMIYAMMGIGLIGFLVWAHHMFTIGMDVDTRAYFTA 304
gi 13383262 225 PILFQHIFWFFGHPEVYILILPGFGMISQIVMFYsgkDSAFGYMGMVYAILGIGFLGFIVWAHHMYTVGLDIDTRAYFTG 304
gi 27228455 237 PILYQHLFWFFGHPEVYILILPGFGMISHVIVFYsgkDNIFGYYGMVWAMGGIGFLGFLVWAHHMFSVGMDVDSRAYFTS 316
gi 57157785 227 PILYQHLFWFFGHPEVYILILPGFGIVSHTVAYYsgkKEVFGYYGMVWAMLGIGFLGFLVWAHHMYTVGMDTDSRAYFTA 306
gi 7335691  230 PVLYQHLFWLFGHPEAYILILPGFGMISHIIVFYtnkEVVFGFYGMVWAIIGIGFLGFLVWAHHMFTVGMDVDSRAYFTA 309
gi 40363500 230 MILFQHLFWFFGHPEVYILILPGFGLVSHIVIHHsskGLAFGTLGMIYAMCAIGLLGFIVWGHHMFTVGLDVDTRAYFTS 309
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                              #        
2OCC_N      308 ATMIIAIPTGVKVFSWLATLHGGnik---wsPAMMWALGFIFLFTVGGLTGIVLANSSLDIVLHDTYYVVAHFHYVLSMG 384
1AR1_A      343 ATMTIAVPTGIKVFSWIATMWGGsie---fkTPMLWAFGFLFLFTVGGVTGVVLSQAPLDRVYHDTYYVVAHFHYVMSLG 419
gi 67077799 305 ATMVIAIPTGVKVFNWIASLSGAyvm---ktPALLWAVGFVFLFTIGGLTGIVLANSSLDVVLHDTYYVVAHFHYVLSMG 381
gi 62184511 309 ATMTIAVPTGIKVFSWAATIFGGkir---wtVPMFWAAGFIYLFTLGGLTGIMLSNSSLDTMLHDTYYVVAHFHYVLSMG 385
gi 51980098 305 ATMIIAVPTGIKVFSWLATIHGSkik---ydTPMLWVLGFIFLFTVGGLTGIVVGNCSLDIMMHDTYYVVAHFHYVLSLG 381
gi 13383262 305 ATMIIAVPTGIKIFSWLATFYGRplakdednVGPLWATGFIFLFTLGGLTGVVLASASLDISLHDTYYVVAHFHYVLSMG 384
gi 27228455 317 ATMIIAVPTGIKVFSWVSTLLGAkih---wgLPLLWAYGFLFLFTIGGLTGIVLANCSLDLVLHDTYYVVAHFHYVLSMG 393
gi 57157785 307 ATMVIGVPTGIKVFSWMGTMAGGrld---wgISFYWTAGFLFLFTIGGLTGIVLSNSSMDLVLHDTYYVVAHFHYVLSMG 383
gi 7335691  310 ATMVIAVPTGIKVFSWVSTLVGAkiv---whIAMYWVCGFLFLFTLGGLTGIILSNCSLDLVLHDTYYVVAHFHYVLSMG 386
gi 40363500 310 ATMIIAVPTGVKIFSWLSTLSGAnfn--nitPSLSWACGFIVLFTFGGFTGIVLSNSSIDLALHDTYYVVAHFHYVLSMG 387
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
2OCC_N      385 AVFaIMGGFVHWFPLFSGYTLnDTWAKIHFAIMFVGVNMTFFPQHFLGLSGMPRRYSDYPDa-YTMWNTISSMGSFISLT 463
1AR1_A      420 AVFgIFAGVYYWIGKMSGRQYpEWAGQLHFWMMFIGSNLIFFPQHFLGRQGMPRRYIDYPVe-FAYWNNISSIGAYISFA 498
gi 67077799 382 AVFaIFGGIALWVPILFSIHLsQKQMKAHFFSTFLGVNLTFFPQHFLGLNGMPRRYSDFPDg-LTFWNSISSFGALLSAL 460
gi 62184511 386 AVFaIMEGLVHFHAMLTGYSLnSTLAKAQFWAMFTGVNITFFPQHMLGLAGMPRRYSDFPDa-YAIWNTISSMGSIMSLV 464
gi 51980098 382 AVFaIFGGLTHYFPLFTGVTLhSRWSKSHFFMMFTGVNLTFFPQHFLGLSGMPRRYSDYPDv-YTTWNVLSSIGAFISFT 460
gi 13383262 385 AVFsIFAGLVHWWVIFTGVALnSKMASAQFWIMFTGVNLTFFPQHFLGLSGMPRRYSDYPDg-FAYWNSISSFGSLVSVI 463
gi 27228455 394 AVFaIFSGFFHWSPLFTGLSFnLILGQIHFFMMFVGVNLTFFPQHFLGLSGMPRRYNDYPDf-YLTWNVVSSIGSLLSGV 472
gi 57157785 384 GVFaVFAGFTHWYPVFTGLSFnKILGEAHFYTTFIGVNLTSFPQHFLGLTGMPRRYNDFPDf-FLAWNTVSSVGSMVSVF 462
gi 7335691  387 AVFaIFAGFAHWYSLFTGLQMsHYLGLVQFWVMFIGVNLTFFPQHFLGLAGMPPRRYNDFSdfYLFWKVASSIGSLMSSL 466
gi 40363500 388 AVFaILGGIVHWFPLFTGYTLnNKYANWQFILMFLGVNLTFFPQHFLGLSGMPRRYTDYPDi-YATWHFISSVGSLISTV 466
                       490       500       510       520
                ....*....|....*....|....*....|....*....|..
Feature 1                                                 
2OCC_N      464 AVMLMVFIIWEAFaskrevlt---------vdltttnlEWLN 496
1AR1_A      499 SFLFFIGIVFYTLfagkrvnvpn------ywnehadtlEWTL 534
gi 67077799 461 SAFFFLWILWEGSvvsrktis---------hnisyssgEWAQ 493
gi 62184511 465 SALTMIVILWEAFskkrsttp---------attdaklqEWTQ 497
gi 51980098 461 SLMFFIFLMWEALasqrgvla---------sphmptalEWQE 493
gi 13383262 464 GVVFFIVIVWESIlservvly---------vispnsqlEWIG 496
gi 27228455 473 AVVVFFLMLWESFyskrflftveskslefklgfppalhTWMT 514
gi 57157785 463 GVVLFLVMLWESMtvmrfastyeld-alefaevppqahTWME 503
gi 7335691  467 GALLFGCMIWESMisnrkvya---------aeetgsylEWSC 499
gi 40363500 467 ALILFVVLLWEALiacrpvmf---------tthpstniEWSH 499

Citing CDD
Marchler-Bauer A et al. (2013), "CDD: conserved domains and protein three-dimensional structure.", Nucleic Acids Res. 41(D1):D384-52.
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Conserved site includes 4 residues -Click on image for an interactive view with Cn3D