Conserved Protein Domain Family
Heme_Cu_Oxidase_I

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cd00919: Heme_Cu_Oxidase_I 
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Heme-copper oxidase subunit I. Heme-copper oxidases are transmembrane protein complexes in the respiratory chains of prokaryotes and mitochondria which catalyze the reduction of O2 and simultaneously pump protons across the membrane. The superfamily is diverse in terms of electron donors, subunit composition, and heme types. The number of subunits varies from three to five in bacteria and up to 13 in mammalian mitochondria. It has been proposed that Archaea acquired heme-copper oxidases through gene transfer from Gram-positive bacteria. Membership in the superfamily is defined by subunit I, which contains a heme-copper binuclear center (the active site where O2 is reduced to water) formed by a high-spin heme and a copper ion. It also contains a low-spin heme, believed to participate in the transfer of electrons to the binuclear center. Only subunit I is common to the entire superfamily. For every reduction of an O2 molecule, eight protons are taken from the inside aqueous compartment and four electrons are taken from the electron donor on the opposite side of the membrane. The four electrons and four of the protons are used in the reduction of O2; the four remaining protons are pumped across the membrane. This charge separation of four charges contributes to the electrochemical gradient used for ATP synthesis. Two proton channels, the D-pathway and K-pathway, leading to the binuclear center have been identified in subunit I of cytochrome c oxidase (CcO) and ubiquinol oxidase. A well-defined pathway for the transfer of pumped protons beyond the binuclear center has not been identified. Electron transfer occurs in two segments: from the electron donor to the low-spin heme, and from the low-spin heme to the binuclear center. The first segment can be a multi-step process and varies among the different families, while the second segment, a direct transfer, is consistent throughout the superfamily.
Statistics
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PSSM-Id: 238461
View PSSM: cd00919
Aligned: 223 rows
Threshold Bit Score: 201.22
Threshold Setting Gi: 56477505
Created: 10-Apr-2003
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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  next features
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Binuclear center (active site) [active site]
Evidence:
  • Comment:The high-spin heme and CuB form the binuclear center, where O2 is reduced to water.
  • Structure:1AR1_A: H411 is the axial ligand of heme a3; CuB ligands are H276, H325, and H326
    View structure with Cn3D

cd00919 is part of a hierarchy of related CD models.
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cd00919 is a member of the superfamily cl00275.
cd00919:Heme_Cu_Oxidase_Icd01660:ba3-like_Oxidase_Icd01661:cbb3_Oxidase_Icd01662:Ubiquinol_Oxidase_Icd01663:Cyt_c_Oxidase_I1EHK A66797955112550631655451355230686112550652309920146113811156133022298917127379591538034921460152218312579156071242301382656477505302486921346009379636726797841130213141342276056708974162627734591590132474205175492144877069956477059679093871592083115897014172943415920898159229301589938042794897159203141592272732820616686392552317161317336748478376159202827304471589968342794899181600351578985055230797552304391FFT F211074103035117833600270461313224757564813475707134694020378344500855115670743654296266295416164568425427377826464468244923929646451249244342230997094416094231029755647552016264696564780123247309246193350134884746792642622988856229893737404346230988931017523551893235679295261561018025028974854694550842190509551292847625251191339995329456561144252191046131224537947276793199410185096668555643244820666857918559802816460449163305632312723017131825226520513363903837520309375217322265204417130297567515169885829336331721347115146314387543020674885547067934493530616689285864521280140762911700030370151085738235828912248331122483501224832755583305621612685158835835446131957464914726372124573432877218157452151285931AR1 A1M56 A61101788462038091347622623014223526988534252018734101909175450825200766530249019538049425429889423061826211102924172267210444217114659271169722168954177110461827509875249757629761271879441603666273622939156485023408554407811690271169030116903113521324106949114665591146660149147171273852423630292585785886647494226534433121889426321855533824113383262272284555715778573356912OCC N51450927116971117002213624421169975200043058352353121888519800984635843249146904621845114914742227262533670777991169631264195212642024126419681232858952220942518300645183005049147370403635001EHK A66797955112550631655451355230686112550652309920146113811156133022298917127379591538034921460152218312579156071242301382656477505302486921346009379636726797841130213141342276056708974162627734591590132474205175492144877069956477059679093871592083115897014172943415920898159229301589938042794897159203141592272732820616686392552317161317336748478376159202827304471589968342794899181600351578985055230797552304391FFT F211074103035117833600270461313224757564813475707134694020378344500855115670743654296266295416164568425427377826464468244923929646451249244342230997094416094231029755647552016264696564780123247309246193350134884746792642622988856229893737404346230988931017523551893235679295261561018025028974854694550842190509551292847625251191339995329456561144252191046131224537947276793199410185096668555643244820666857918559802816460449163305632312723017131825226520513363903837520309375217322265204417130297567515169885829336331721347115146314387543020674885547067934493530616689285864521280140762911700030370151085738235828912248331122483501224832755583305621612685158835835446131957464914726372124573432877218157452151285931AR1 A1M56 A61101788462038091347622623014223526988534252018734101909175450825200766530249019538049425429889423061826211102924172267210444217114659271169722168954177110461827509875249757629761271879441603666273622939156485023408554407811690271169030116903113521324106949114665591146660149147171273852423630292585785886647494226534433121889426321855533824113383262272284555715778573356912OCC N5145092711697111700221362442116997520004305835235312188851980098463584324914690462184511491474222726253367077799116963126419521264202412641968123285895222094251830064518300504914737040363500
cd00919 Sequence Cluster
cd00919 Sequence Cluster
Sub-family Hierarchy
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CD Hierarchy
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AR1_A       28 HKDIGILYLFTAGIVGLISVCFTVYMRMELqhpgvqymclegarl----------iadasaectpnghlWNVMITYHGVL 97
1EHK_A       17 EKKATLYFLVLGFLALIVGSLFGPFQALNYgnvdaypll---------------------krllpfvqsYYQGLTLHGVL 75
1FFT_F       54 HKRLGIMYIIVAIVMLLRGFADAIMMRSQQalasagea------------------------gflpphhYDQIFTAHGVI 109
1M56_A       26 HKDIGVLYLFTGGLVGLISVAFTVYMRMELmapgvqfmcaehlesglvkgffqslwpsavenctpnghlWNVMITGHGIL 105
2OCC_N       12 HKDIGTLYLLFGAWAGMVGTALSLLIRAELgqpgtl---------------------------lgddqiYNVVVTAHAFV 64
gi 1346009   24 AHDSLFAAHMWVLFFTLVVSTLLLLRRVSFlppvagp--------------------------pcrrteYFDEVVKYGVM 77
gi 37963672  25 AADFGFAVHMGIVCAAALAVAVLTMRHADYeaitrgll-----------------------kmpasqgrYDDDPIRWGVI 81
gi 67978411  24 GLDLAYRVHAVLIMLVAGGLFLWQVRQTGEervpa------------------------------pqgeYMDGPVRAAAI 73
gi 3021314   35 ALDAPFAFHAALSAAASVAAVFCIVNRYFErpaalppa------------------------eingrpnYNMGPIKFSSF 90
gi 13422760  36 THDPMFRLQIGTFALAGVIAAFALNAGLAGgtlqd------------------------------hpdeYSDNVVKAGVI 85
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AR1_A       98 MMFFVVipaLFGGFGNyfmplhigapDMAFPR---LNNLSYWMYVCGVALGVASLlapggndqmgsgVGWVLYPPLstte 174
1EHK_A       76 NAIVFTqlfAQAIMVYlpar---elnMRPNMG---LMWLSWWMAFIGLVVAALPLlan------eatVLYTFYPPLkg-- 141
1FFT_F      110 MIFFVAmpfVIGLMNLvvplq-igarDVAFPF---LNNLSFWFTVVGVILVNVSLgvge-----faqTGWLAYPPLsgie 180
1M56_A      106 MMFFVVipaLFGGFGNyfmplhigapDMAFPR---MNNLSYWLYVAGTSLAVASLfapggngqlgsgIGWVLYPPLstse 182
2OCC_N       65 MIFFMVmpiMIGGFGNwlvplmigapDMAFPR---MNNMSFWLLPPSFLLLLASSmvea-----gagTGWTVYPPLagnl 136
gi 1346009   78 ATVFWGvvgFLVGVVVa--------lQLAFPD---LNIAPYFNFGRMRPLHTSAVifafg---gnalIATSFYVVQrtcr 143
gi 37963672  82 ATVFWGlagFLAGLYIa--------lQLAFPAl--NLGLEYTSFGRLRPLHTSAVifafg---gnalIATSFYVVQrtcr 148
gi 67978411  74 ATVFWGctgFLVGVVIa--------fQLAFPVlnfEWAQPYLNFGRLRPLHTSAVifafg---gnalIAATFYIGQrtca 142
gi 3021314   91 MAMFWGiagFLVGLIIa--------sQLAWPAl--NFDLPWISFGRLRPLHTSAVifafg---gnvlIATSFYVVQkscr 157
gi 13422760  86 ATMFWGaagLLVGVIIa--------aQLSWPTifyFPDLGFLNFGRLRPLHTSAVifafg---gnalIATSFYVVQrtck 154
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1AR1_A      175 a----gysmDLAIFAVHVSGASSILGAINIITTFLnmrapgmtlfkvpLFAWSVFITaWLILLSLPVLAGAITMLLMDRn 250
1EHK_A      142 --------hWAFYLGASVFVLSTWVSIYIVLDLWRrwkaan-pgkvtpLVTYMAVVFwLMWFLASLGLVLEAVLFLLPWs 212
1FFT_F      181 ys--pgvgvDYWIWSLQLSGIGTTLTGINFFVTILkmrapgmtmfkmpVFTWASLCAnVLIIASFPILTVTVALLTLDRy 258
1M56_A      183 s----gystDLAIFAVHLSGASSILGAINMITTFLnmrapgmtmhkvpLFAWSIFVTaWLILLALPVLAGAITMLLTDRn 258
2OCC_N      137 ah--agasvDLTIFSLHLAGVSSILGAINFITTIInmkppamsqyqtpLFVWSVMITaVLLLLSLPVLAAGITMLLTDRn 214
gi 1346009  144 arlfggnlgWFVFWGYNLFIIMAATGYLLGITQGReyaepewyvdlwlTIVWVAYLAtFLGTILTRKEPHISVANWFYLs 223
gi 37963672 149 arlafpalaRFVFWGYQLFIVLAATGYLLGITEGKeyaepewyvdlwlTIVWVAYLVvFGGTILRRSEPHIYVANWFYLs 228
gi 67978411 143 vrmwggnlsWFVFWGYQFFIVLAATGYLLGATQSLeyaepewyvdlwlTIVWVAFLIvYMGTILTRKEPHIYVANWFLLa 222
gi 3021314  158 vrlagdlapWFVVVGYNFFILVAGTGYLLGVTQSKeyaepewyadlwlTIVWVVYLLvFLATIIKRKEPHIFVANWFYLa 237
gi 13422760 155 arlaggitpWFVFWGYQLFIVLAATGYLMGATSGKeysepewytdlwlTIVWVAYLLvFLGTIWKRKEPHIYVANWFYLa 234
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                 #                                     
1AR1_A      251 fgtqff-----------------dpagggDPVLYQHILWFFGHPEVYIIILPGFGIISHVISTFak-kPIFGYLPMVLAM 312
1EHK_A      213 fgl----------------------vegvDPLVARTLFWWTGHPIVYFWLLPAYAIIYTILPKQag-gKLVSDPMARLAF 269
1FFT_F      259 lgthff-----------------tndmggNMMMYINLIWAWGHPEVYILILPVFGVFSEIAATFsr-kRLFGYTSLVWAT 320
1M56_A      259 fgttff-----------------qpsgggDPVLYQHILWFFGHPEVYIIVLPAFGIVSHVIATFak-kPIFGYLPMVYAM 320
2OCC_N      215 lnttff-----------------dpagggDPILYQHLFWFFGHPEVYILILPGFGMISHIVTYYsgkkEPFGYMGMVWAM 277
gi 1346009  224 fivtiamlhivnnlavpvsflgvksysafSGVQAALTQWWYGHNAVGFFLTAGFLGMMYYFIPKqvnrPVYSYRLSIIHF 303
gi 37963672 229 filtiamlhivnnlsmpvswlgsksysafAGVQDALTQWWYGHNAVGFFLTAGFLAMMYYFVPKqaerPVYSYRLSIIHF 308
gi 67978411 223 fvitvamlhlinnmavpvsifgsrsvqvlSGVQSAMTQWWYGHNAVGFFLTAGFLGMMYYFVPKqaerPVYSYKLSIIHF 302
gi 3021314  238 fivtiavlhlgnnpalpvsafgsksyvawGGIQDAMFQWWYGHNAVGFFLTAGFLAIMYYFIPKraerPIYSYRLSIIHF 317
gi 13422760 235 fivtvallhvvnnaavpvgllnhksygvfAGVQDALVQWWYGHNAVGFFLTAGFLAMMYYFVPRrverPVYSYRLSIVHF 314
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   ##                                                                  
1AR1_A      313 AAIGILGFVVWAHHMYTAGms-ltQQAYFMLATMTIAVPTGIKVFSWIATMWGgsi---------------efkTPMLWA 376
1EHK_A      270 LLFLLLSTPVGFHHQFADPgidptWKMIHSVLTLFVAVPSLMTAFTVAASLEFagrlrggrglfgwiralpwdnPAFVAP 349
1FFT_F      321 VCITVLSFIVWLHHFFTMGag-anVNAFFGITTMIIAIPTGVKIFNWLFTMYQgri---------------vfhSAMLWT 384
1M56_A      321 VAIGVLGFVVWAHHMYTAGls-ltQQSYFMMATMVIAVPTGIKIFSWIATMWGgsi---------------elkTPMLWA 384
2OCC_N      278 MSIGFLGFIVWAHHMFTVGmd-vdTRAYFTSATMIIAIPTGVKVFSWLATLHGgni---------------kwsPAMMWA 341
gi 1346009  304 WALIFMYIWAGPHHLHYTAlp-dwAQTLGMVFSIMLWMPSWGGMINGLMTLSGawdk-------------irtdPIVRMM 369
gi 37963672 309 WSLIFLYIWAGPHHLHYTAlp-dwAQTLGMVFSVMLWMPSWGGMINGLMTLNGawdr-------------irtdPIIRMM 374
gi 67978411 303 WALIFLYIWAGPHHLHYTAlp-dwAATLGMVFSIVLWMPSWGGMINGLMTLQGawdk-------------lrtdPILRMM 368
gi 3021314  318 WALIFLYIWAGPHHLHYTAlp-dwTQTLGMTFSIMLWMPSWGGMINGLMTLSGawdk-------------lrtdPVLRML 383
gi 13422760 315 WALIFIYIWAGPHHLHYTAlp-qwAQTLGMTFSIMLWMPSWGGMINGLMTLSGawdk-------------lrtdPVVRMM 380
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         #                                             
1AR1_A      377 FGFLFLFTVGGVTGVVLsqapLDRVYHDTYYVVAHFHYVMSLGAVfgIFAGVYYWIGKMSgrqy---pewAGQLHFWMMF 453
1EHK_A      350 VLGLLGFIPGGAGGIVNasftLDYVVHNTAWVPGHFHLQVASLVTltAMGSLYWLLPNLTgkpisdaqrrLGLAVVWLWF 429
1FFT_F      385 IGFIVTFSVGGMTGVLLavpgADFVLHNSLFLIAHFHNVIIGGVVfgCFAGMTYWWPKAFgfkl---netWGKRAFWFWI 461
1M56_A      385 LGFLFLFTVGGVTGIVLsqasVDRYYHDTYYVVAHFHYVMSLGAVfgIFAGIYFWIGKMSgrqy---pewAGKLHFWMMF 461
2OCC_N      342 LGFIFLFTVGGLTGIVLanssLDIVLHDTYYVVAHFHYVLSMGAVfaIMGGFVHWFPLFSgytl---ndtWAKIHFAIMF 418
gi 1346009  370 VMAVAFYGMATFEGPMMsikaVNSLSHYTDWTIGHVHSGALGWNGmiTFGAIYYLTPKLWgrdrl-yslqLVNWHFWLAT 448
gi 37963672 375 VMALAFYGMSTFEGPMMsvkaVNSLSHYTDWTIGHVHSGALGWNGliTFACLYYLTPRLWkrerl-yslrLVNWHFWLAT 453
gi 67978411 369 VTSLAFYGMSTFEGPMMsiraVNSLSHYTDWTIGHVHSGALGWNGmiTFGMLYYLFPKLWhtkl--ysmrAVSWHFWLAT 446
gi 3021314  384 VVSVAFYGMSTFEGPMMsikvVNSLSHYTDWTIGHVHSGALGWVGfvSFGALYCLVPWAWnrkgl-yslkLVNWHFWVAT 462
gi 13422760 381 VVSIAFYGMSTFEGPVMsiraVNALSHYTDWTIGHVHSGALGWVGfiSFGAIYCLVPWLWkkpgm-ysnkLIEWHFWIAT 459
                       490       500       510       520       530       540
                ....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1                                                                       
1AR1_A      454 IGSNLIFFPQHFLgr-qGMPRRYidypv-----efaywNNISSIGAYISFASFLFFIGIVFYTL 511
1EHK_A      430 LGMMIMAVGLHWAgl-lNVPRRAyiaqvpdayphaavpMVFNVLAGIVLLVALLLFIYGLFSVL 492
1FFT_F      462 IGFFVAFMPLYALgf-mGMTRRLsqqidp----qfhtmLMIAASGAVLIALGILCLVIQMYVSI 520
1M56_A      462 VGANLTFFPQHFLgr-qGMPRRYidype-----afatwNFVSSLGAFLSFASFLFFLGVIFYTL 519
2OCC_N      419 VGVNMTFFPQHFLgl-sGMPRRYsdypd-----aytmwNTISSMGSFISLTAVMLMVFIIWEAF 476
gi 1346009  449 LGIVVYAAVMWVAgiqqALMWREydsqg------flvySFAESVAALFPYYVMRALGGLMFLSG 506
gi 37963672 454 VGIVLYAASMWVAgimqGLMWREygsdg------ylvyAFSEVVSAMFPMYLIRAAGGLLYLSG 511
gi 67978411 447 VGIVLYAASMWVTgimeGLMWREvdaqg------flvnSFADTVEAKFPMYVVRGLGGVMFLSG 504
gi 3021314  463 LGIVLYISAMWVSgilqGLMWRAytslg------fleySFIETVEAMHPFYIIRAAGGGLFLIG 520
gi 13422760 460 TGILLYIAAMWVSgvmeGLMWREytpqg------flaySFIETVSAKHIENVIRTVGGLMYLSG 517

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Conserved site includes 4 residues -Click on image for an interactive view with Cn3D