1DTD,2V77,2BO9,2PCU,2BOA


Conserved Protein Domain Family
M14_CPA

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cd03870: M14_CPA 
Click on image for an interactive view with Cn3D
Peptidase M14 carboxypeptidase subfamily A/B-like; Carboxypeptidase A subgroup
Peptidase M14 Carboxypeptidase (CP) A (CPA) belongs to the A/B subfamily of the M14 family of metallocarboxypeptidases (MCPs). The M14 family are zinc-binding CPs which hydrolyze single, C-terminal amino acids from polypeptide chains, and have a recognition site for the free C-terminal carboxyl group, which is a key determinant of specificity. CPA enzymes generally favor hydrophobic residues. A/B subfamily enzymes are normally synthesized as inactive precursors containing preceding signal peptide, followed by a globular N-terminal pro-region linked to the enzyme; these proenzymes are called procarboxypeptidases. The procarboxypeptidase A (PCPA) is produced by the exocrine pancreas and stored as a stable zymogen in the pancreatic granules until secretion into the digestive tract occurs. This subfamily includes CPA1, CPA2 and CPA4 forms. Within these A forms, there are slightly different specificities, with CPA1 preferring aliphatic and small aromatic residues, and CPA2 preferring the bulkier aromatic side chains. CPA4, detected in hormone-regulated tissues, is thought to play a role in prostate cancer.
Statistics
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PSSM-Id: 349442
Aligned: 13 rows
Threshold Bit Score: 490.796
Created: 18-Oct-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding siteactive siteinhibitor
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: Zn binding site [ion binding site], 3 residue positions
Conserved feature residue pattern:[H] [ED] [H]Click to see conserved feature residue pattern help
Evidence:
  • Comment:Metallocarboxypeptidases share the zinc binding motif HXXE...H, where the zinc ion is penta-coordinated to ND1 atoms of the histidines, OE1 and OE2 atoms of the glutamic acid, and to a water molecule in a slightly distorted tetrahedral manner.
  • Structure:1DTD_A: human Carboxypeptidase A2; contact distance 3.5A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1DTD_A      1 FNFG------------AYHTLE----------------------EISQEMDNLVAEHPg----LVSKVNIGSSF--ENRP 40  human
EUB53850   77 FNFG------------AYHTLE----------------------EISQEMENLVAEYPs----LVSQVNIGSSF--ENRP 116 Echinococcus granu...
2V77_A      7 FNYA------------TYHTLE----------------------EIYDFLDLLVAENPh----LVSKIQIGNTY--EGRP 46  human
NP_989915 119 FDYT------------SYHTLE----------------------EIYAFMDLLVAENPn----LVSKLEIGRTT--ENRP 158 chicken
2BO9_A      5 FNYG------------AYHSLE----------------------AIYHEMDNIAADFPd----LARRVKIGHSF--ENRP 44  human
2PCU_A      3 FNYG------------AYHSLE----------------------AIYHEMDNIAADFPd----LARRVKIGHSF--ENRP 42  human
2BOA_A    101 FNYG------------AYHSLE----------------------AIYHEMDNIAADFPd----LARRVKIGHSF--ENRP 140 human
NP_653120 134 FSYS------------SYHTLD----------------------EIYSWIDNFVAEHSn----LVSKIHIGKSF--ENRS 173 house mouse
AAH84831  119 FNYA------------TYHTFD----------------------EINAFIDNLVNENPn----LVSKIQIGSSY--EGRP 158 African clawed frog
AAH76057  117 FNFA------------AYHDLD----------------------TIYSFMDTLVASHSn----LISKVKIGSTY--ENRP 156 zebrafish
OXB58047   15 FSEQl----------fVGDQVLrvvasneeqiallrelgeqvdlQIDFWRDPTKPGRPadlrvPFPRLQAVKVFleSNGI 84  scaled quail
KFO29347   24 CSFIleaaw--gqmncAGHQVLrvlakdeeqlsllrdlenlktqKVDFWWGPARPSLPvdmrvPFSELDDIKADleSHGL 101 Fukomys damarensis
EPY81141    6 FFGAligsgicgrekcFGDQVFrinvrngdeiskisqlvnsdnfKLSFWKSPSTFGHPvdilvPSVSLQPVRAFlkSQLL 85  Wild Bactrian camel
Feature 1                               #  #                                                  
1DTD_A     41 MNVLKFSTGg-----dKPAIWLDAGIHAREWVTQATALWTANKIVSDYGkd-pSITSILDaLDIFLLP-VTNPDGYVFSQ 113 human
EUB53850  117 MNVLKFSTGg-----dKPAIWLDAGIHAREWVTQATALWTANKIASDYGkd-pSITSLLDtMDIFLLP-VTNPDGYVFSQ 189 Echinococcus granu...
2V77_A     47 IYVLKFSTGgs----kRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGqd-aAFTAILDtLDIFLEI-VTNPDGFAFTH 120 human
NP_989915 159 IYVLKFSKGgt----nRPAIWIDTGIHSREWVTQASGIWFAKKIVQEQDe---GLANILDqMDIFLEI-VTNPDGFAFTH 230 chicken
2BO9_A     45 MYVLKFSTGkg---vrRPAVWLNAGIHSREWISQATAIWTARKIVSDYQrd-pAITSILEkMDIFLLP-VANPDGYVYTQ 119 human
2PCU_A     43 MYVLKFSTGkg---vrRPAVWLNAGIHSREWISQATAIWTARKIVSDYQrd-pAITSILEkMDIFLLP-VANPDGYVYTQ 117 human
2BOA_A    141 MYVLKFSTGkg---vrRPAVWLNAGIHSREWISQATAIWTARKIVSDYQrd-pAITSILEkMDIFLLP-VANPDGYVYTQ 215 human
NP_653120 174 ILVLKFSTGgp----nRPAIWIDTGIHSREWITHATGIWISQKIVNAYGkd-hVLKRILNtMDIFIEI-VTNPDGFAFTH 247 house mouse
AAH84831  159 LNVLKFSTGp-----nRPAFWIDTGIHSREWVTQASGIWFAKKIATDYGkd-aSLTNTLNnIDILLEI-VTNPDGYVYTH 231 African clawed frog
AAH76057  157 MYVLKFSTGge----nKPAIWIDAGIHAREWVSHASAVWIADRIATDFKenraPVPDVLSkMDIYLMI-VANPDGYVFSH 231 zebrafish
OXB58047   85 SYSIMIEDVqqlldeeKRTMMLSRRMERSVSKFNFASYHTLEEFAEDYGhd-pTVTSLLDtVDIFFEI-VTNPDGYAFTH 162 scaled quail
KFO29347  102 VYSVMIKNVqelldqeREAMAKAHQLERSTSSFSYSSYHTLEEIVSEYGkd-hILTDILKvMDIFIEL-VTNPDGFALTH 179 Fukomys damarensis
EPY81141   86 EYSVTIEDLqalldneEKERQHNEGQERSSNNFNYGAYHSLEAIYHEMD----SIAQDFSgLVSRVKIgHSFENRSMYIL 161 Wild Bactrian camel
Feature 1                                                                                     
1DTD_A    114 t------------------kNRMWRKTRSKvsagslCVGVDPNRNWDAGFGgpGASSNPCSDSYHGPSANSEVEVKSIVD 175 human
EUB53850  190 t------------------kNRMWRKTRSKvsg-slCVGVDPNRNWDAGFGgpGASKQPCSDSYHGPSANSEVEVKSIVD 250 Echinococcus granu...
2V77_A    121 s------------------tNRMWRKTRSHtag-slCIGVDPNRNWDAGFGlsGASSNPCSETYHGKFANSEVEVKSIVD 181 human
NP_989915 231 t------------------sNRMWRKTRSKrsg-slCVGVDPNRNWDAGFGgsGASSNPCSETYHGPYANSEPEVKAIVD 291 chicken
2BO9_A    120 t------------------qNRLWRKTRSRnpg-ssCIGADPNRNWNASFAgkGASDNPCSEVYHGPHANSEVEVKSVVD 180 human
2PCU_A    118 t------------------qNRLWRKTRSRnpg-ssCIGADPNRNWNASFAgkGASDNPCSEVYHGPHANSEVEVKSVVD 178 human
2BOA_A    216 t------------------qNRLWRKTRSRnpg-ssCIGADPNRNWNASFAgkGASDNPCSEVYHGPHANSEVEVKSVVD 276 human
NP_653120 248 s------------------mNRLWRKNKSSqpg-ifCIGVDLNRNWKAGFGgnGSNKNPCSETYRGPAPESEPEVAAIVD 308 house mouse
AAH84831  232 k------------------sDRMWRKTRSPnsg-slCVGTDPNRNWDAGFGgpGSSSNPCTETYRGRAAHSEPEVKAIVD 292 African clawed frog
AAH76057  232 ltv--------------rpfHRLWRKSRSVtsn-pnCPGVDLNRNFDAEFSgpGASDDPCAEDYRGPSAHSEIEVTNIVD 296 zebrafish
OXB58047  163 s------------------sNRLWRKTRSVnag-ssCIGVDPNRNWDAGFGdaGSSSNPCSETYHGPYAHSESEVKSIVD 223 scaled quail
KFO29347  180 sttplrqsgwqhshawesgpNRLWRKNKSSrpg-vlCTGVDLNRNWKSDFGgnGSNSNPCSETYHGPLPRSEPEVAAIVD 258 Fukomys damarensis
EPY81141  162 k-------------------DRLWRKSRSLnpg-ssCIGTDLNRNWNVSFAgkGASDDPCSEAYHGTHANSEVEVKSVVD 221 Wild Bactrian camel
Feature 1                   #                                                                 
1DTD_A    176 FIKSHGkVKAFIILHSYSQLLMFPYGYKCTKLDDFDELSEVAQKAAQSLSRlhGTKYKVGPICSVIYQASGGSIDWSYDY 255 human
EUB53850  251 FIKSHGkVKAFITIHSYSQLLMFPYGYTCSKPDNFDELNEVAQKAAKSVASlhGTQYKVGPICSVIYQASGGSIDWAYDF 330 Echinococcus granu...
2V77_A    182 FVKDHGnIKAFISIHSYSQLLMYPYGYKTEPVPDQDELDQLSKAAVTALASlyGTKFNYGSIIKAIYQASGSTIDWTYSQ 261 human
NP_989915 292 FVKSHKnIKAFISIHSYSQLLLYPYGYTTTAVPDKEELHQVAKEAVAALSSlyGTNYKYGSIITTIYQASGGTIDWTYNQ 371 chicken
2BO9_A    181 FIQKHGnFKGFIDLHSYSQLLMYPYGYSVKKAPDAEELDKVARLAAKALASvsGTEYQVGPTCTTVYPASGSSIDWAYDN 260 human
2PCU_A    179 FIQKHGnFKGFIDLHSYSQLLMYPYGYSVKKAPDAEELDKVARLAAKALASvsGTEYQVGPTCTTVYPASGSSIDWAYDN 258 human
2BOA_A    277 FIQKHGnFKGFIDLHSYSQLLMYPYGYSVKKAPDAEELDKVARLAAKALASvsGTEYQVGPTCTTVYPASGSSIDWAYDN 356 human
NP_653120 309 FITGHGnFKAMISIHSYSQMVMYPYGHSLEPVPNHEELFNLAKDAVKALNKvhGIQYIFGSISTTLYSASGISVDWAYDS 388 house mouse
AAH84831  293 FVKSHGnIKGFVSIHSYSQMLLFPYGYTTARTPDHNELNTVSRNAVNALASlyGTSYTYGSIIATIYQASGGTIDWTYNQ 372 African clawed frog
AAH76057  297 FILSHGnFKSFMTLHAYSQLLMYPYGYTGSNAPDQPELHDVATQANKALNSwhGTIYKVGSIFHTIEQASGASVDWAYQH 376 zebrafish
OXB58047  224 FIQSHGnVKALISIHSYSQMLMFPYGYKRTPAPNHEELNELAKKAVSDLAAvyGTEYTYGSIADTIYLAAGTTVDWAYEN 303 scaled quail
KFO29347  259 FITGHGnFKALISIHSYSQMLTYPYGHCVEPISDQTELYNLAKDAVRAMSEvhRIKYLFGSISTTLYVATGVTVDWAYDN 338 Fukomys damarensis
EPY81141  222 FIQKHRnFKCFIDLHSYSQLLMYPYGYTFNKAPDTKELDEVARRAAKALASlsGTEYRVGPISTTIYPASGSSTDWAYDN 301 Wild Bactrian camel
Feature 1                                                   
1DTD_A    256 GIKYSFAFELRDTGRYGFLLPARQILPTAEETWLGLKAIMEHVRDH 301 human
EUB53850  331 GIKYSFAFELRDTGRYGFLLPANQILPTAEETWLGLKTIMEHVRDH 376 Echinococcus granulosus
2V77_A    262 GIKYSFTFELRDTGRYGFLLPASQIIPTAKETWLALLTIMEHTLNH 307 human
NP_989915 372 GIKYSFTFELRDTGRYGFLLPASQIVPTAQETWKALEVIMAHTRGH 417 chicken
2BO9_A    261 GIKFAFTFELRDTGTYGFLLPANQIIPTAEETWLGLKTIMEHVRDN 306 human
2PCU_A    259 GIKFAFTFELRDTGTYGFLLPANQIIPTAEETWLGLKTIMEHVRDN 304 human
2BOA_A    357 GIKFAFTFELRDTGTYGFLLPANQIIPTAEETWLGLKTIMEHVRDN 402 human
NP_653120 389 GIKYAFSFELRDTGQYGFLLPASQIVPTAEETWMALQTIMKHTLNH 434 house mouse
AAH84831  373 GIKYSYSFELRDTGRYGFALPANQIIPTAEETWLALTKLIEHTRDN 418 African clawed frog
AAH76057  377 GIKYCFAFELRDTGEYGFLLPADQIVSTARETWFALRYIMQYVSDH 422 zebrafish
OXB58047  304 GVKYSYTFELRDTGRYGFLLPSSQIIPTATETWPALLDILVHVQQH 349 scaled quail
KFO29347  339 GIKYAFTFELRDTGRYGFLLPATQIIPTTQETWVAMRTIMEHTLNH 384 Fukomys damarensis
EPY81141  302 GIKYAFAFELRDTRHYGFLLPANQIIPTAEETWLGMKTIMEHVRDH 347 Wild Bactrian camel

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