Conserved Protein Domain Family
EFh

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cd00051: EFh 
Click on image for an interactive view with Cn3D
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.
BioAssay Targets and Results
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CID: 44409753
AID: 263483
GI: 115613
IC50: 0.01µM
more
Inhibition of mu calpain in porcine erythrocyte
Target Name: Calpain small subunit 1
Chemical Name: CHEMBL206243
IC50: 0.01µM
CID: 44405281
AID: 257051
GI: 115613
IC50: 0.02µM
more
Inhibitory activity against porcine calpain I using Suc-Leu-Tyr-AMC fluorogenic substrate
Target Name: Calpain small subunit 1
Chemical Name: CHEMBL200826
IC50: 0.02µM
CID: 44409659
AID: 263483
GI: 115613
IC50: 0.015µM
more
Inhibition of mu calpain in porcine erythrocyte
Target Name: Calpain small subunit 1
Chemical Name: CHEMBL378702
IC50: 0.015µM
Statistics
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PSSM-Id: 28933
View PSSM: cd00051
Aligned: 104 rows
Threshold Bit Score: -1
Threshold Setting Gi: 0
Created: 1-Nov-2000
Updated: 9-Mar-2011
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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Ca2+ binding
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:Ca2+ binding site [ion binding site]
Evidence:
  • Structure:1FW4_A, each Ca2+ is bound by four sidechains
    View structure with Cn3D
  • Comment:some sites may be non-functional

cd00051 is part of a hierarchy of related CD models.
Use the graphical representation to navigate this hierarchy.
cd00051:EFh1JF0 A1EJ3 A1171672128012465419548940586237115644115392134566413473411708241170824116880158486946169954909424934621159021BOD  1198581198591168696729092730481729055465834134565911549511295714424461112959131246891351098134749134756117082511699991FW4 A11539014286110115504231700115501115523117690025062531CDL B996732317001155011155331TRF  5490941153905439367290517290522316807290517290521A2X A1DTL A1C7V A11687511352331729092116878211686961TCO B1154951DGU A1BJF A1G8I A117167058515611716714654181JBA A1169874134637113463721170636462436120165250625413467331355782316801RTP 14CPV  5PAL  1RRO  729056115644729056729056134749135109813475613475913476211708251153911JF0 A1EJ3 A1171672128012465419548940586237115644115392134566413473411708241170824116880158486946169954909424934621159021BOD  1198581198591168696729092730481729055465834134565911549511295714424461112959131246891351098134749134756117082511699991FW4 A11539014286110115504231700115501115523117690025062531CDL B996732317001155011155331TRF  5490941153905439367290517290522316807290517290521A2X A1DTL A1C7V A11687511352331729092116878211686961TCO B1154951DGU A1BJF A1G8I A117167058515611716714654181JBA A1169874134637113463721170636462436120165250625413467331355782316801RTP 14CPV  5PAL  1RRO  72905611564472905672905613474913510981347561347591347621170825115391
cd00051 Sequence Cluster
cd00051 Sequence Cluster
Sub-family Hierarchy
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CD Hierarchy
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                       10        20        30        40        50        60
               ....*....|....*....|....*....|....*....|....*....|....*....|....*...
Feature 1               # # #      #                             # # #      #      
1FW4_A       7 EIREAFRVFDKDGNGYISAAELRHVMtnlg-----ekltDEEVDEMIREADIDGDGQVNYEEFVQMMT 69
gi 584869    1 MAEALFKEIDVNGDGAVSYEEVKAFVskkr-----aiknEQLLQLIFKSIDADGNGEIDQNEFAKFYG 63
gi 135578   12 EFIRAFLEIDADSNEMIDKQELIKYCqky-------rldMKLIDPWIARFDTDKDNKISIEEFCRGFG 72
gi 1345664 696 SCRSMIALMDTDGSGKLNLQEFHHLWn-----------kIKAWQKIFKHYDTDQSGTINSYEMRNAVN 752
gi 1168696  40 RLRRIFDLFDKNSDGIITVDELSRALnllg-----letdLSELESTVKSFTREGNIGLQFEDFISLHQ 102
gi 115902  104 VVKGTWGMCDKNADGQINADEFAAWLtal-------gmsKAEAAEAFNQVDTNGNGELSLDELLTAVR 164
gi 231680   69 ELVQAFKLIDRDDDGVVSRGDLAALIsrlsh----eppsQEEVSLMLREVDGGDGGCISLEDLASRVA 132
gi 134734   74 TCRLMVSMLDRDMSGTMGFNEFKELWa-----------vLNGWRQHFISFDSDRSGTVDPQELQKALT 130
gi 461699  403 AASAAFRIHDSNCSGTLSKKEFKKLIkhlg-----yyfsKGQTKMLFHSIDRDYSGSLSEREFVDWWS 465
gi 2493462 207 VVLETLEDIDKNGDGFVDQDEYIADMfsheengpepdwvLSEREQFNEFRDLNKDGKLDKDEIRHWIL 274

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