Conserved Protein Domain Family
XLF

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pfam09302: XLF 
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XLF-Cernunnos, XRcc4-like factor, NHEJ component
XLF (also called Cernunnos) is Xrcc4-like-factor, and interacts with the XRCC4-DNA ligase IV complex to promote DNA non-homologous end-joining. It directly interacts with the XRCC4-Ligase IV complex and siRNA-mediated down-regulation of XLF in human cell lines leads to radio-sensitivity and impaired DNA non-homologous end-joining. This family contains Nej1 (non-homologous end-joining factor), and Lif1, ligase-interacting factor. XLF forms one of the components of the NHEJ machinery for DNA non-homologous end-joining.
Statistics
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PSSM-Id: 286395
View PSSM: pfam09302
Aligned: 89 rows
Threshold Bit Score: 31.1187
Threshold Setting Gi: 207345356
Created: 26-Jun-2015
Updated: 5-Aug-2015
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

pfam09302 is a member of the superfamily cl08542.
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3W03_A        14 PWAWLQLaENS-----LLAKVFIT--KQ--GYAL-LV-SDLQQ--VWHEQVDTSVVSQRAKE---L---NKRL-----T- 68
gi 74608996    4 AWKVGEIdG---------------------RYNL------LKQ------DIDRDGTKLLKITihmT---EALLvpqssTl 47
gi 74644939   14 EWCVKKInGEGn---cLLLFLPMSspTT--IVMI-VL-VSLERlvPYVFKLSQTQLSQQCQSqgfT---DS--------- 74
gi 50401795    2 SWVQLRGhRf------HFIQASFDaeSNe-AVQViHI-TDLVR--VWSCTCSRNQIVNQAES---E---RCPIdp----- 60
gi 754415898   6 KWNVLNIdSPKk----YYYGVDFS--TEkiHYSL-YI-TDFSN--VWISDVDEEKLHFKAIQ---N---G--IetesiN- 66
gi 448083895   7 TWFSTSLsSQGtekvkLLYGYEFSgeSK--SYLI-CT-TDLND--VWIERLNKSQIIKKAKRfgiE---DLS-------- 69
gi 112362763  14 EWFVGNVdGEEnh-lvTFLPISSS--TI--IFTV-VL-VSLNDfvPHVFELTQTQLIQQCQN---Q---Gft-----dN- 74
gi 366992846  15 EWHSCTInGQp-----FLFSLNLSdnEE--STLF-FInADQHP--TFKACISSNDIKAQCTI---QgfpSDSVtkitkE- 80
gi 748452793  14 KWFVGNVnGEEn---hLVALLPMS--SSt-ILTI-VC-VSLNSlvPNVFKLTQNQFLKQCQN---Q---Gft-----dS- 73
gi 207345356 124 VWk-------------VVMELESSaiIR-------KI-AELTLhpVKKGEIDLFEMADKLYK---D---ICCVndsyrNi 176
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3W03_A        69 ----A-----PPAAFLCHLDNLLrpl-----lkDAAHPSe-----A-------TFSCD--------CvaD------A--- 105
gi 74608996   48 kkslK-----TYAVKLDSARKQLksqgfdlqssDNLIKIvieflnN-------LNCYTknnykdskCemSlhksdmK--- 112
gi 74644939   75 ----I-----SLNLIKLKLMDIL----------QAPQEInqiglvD-------SNLVF------------------S--- 107
gi 50401795   61 ---fQ-----TFETLVDVLAEVLvnvs-----------------------------------sesrIqlRgsndegS--- 94
gi 754415898  67 ----G-----VYHTLISTFENNF----------NSEDVEt-----R-------VDMNE--------MkiD------Kgip 101
gi 448083895  70 ----D-----KVEDLLAVFSEGF----------GSRSKNk-----T-------LKFE---------IndQ------S--- 100
gi 112362763  75 ----T-----SSNQIKLKLMDIL----------QSPKKIs-----Q-------FKLVD--------Sn-------lK--- 105
gi 366992846  81 ----TykqihQLRSF-KKIEDGs----------------------R-------LQLTI--------VlsG------D--- 109
gi 748452793  74 ----T-----SLNQIRLKLIDIL----------QFPKKIs-----KfgiidskLNFNF--------SisA------N--- 112
gi 207345356 177 kesdS-----SNRNRVEQLARER-----------------------------------------------------E--- 195
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
3W03_A       106 L--ILRV------RS-----------------ELSGL--PFYWNFHCM--LASPS---L---VSQHLIRPLMG---MSLA 147
gi 74608996  113 I--TIKLdatikaTInlgfvrltdeqgllillELSHFlsETLWYWSRVcsLIDSE---S---NTKNLNNCLI-------- 176
gi 74644939  108 F--DVSA--------------------------------DITVSINSVpsHVTKD---MfymILQSLCMLLLK---LVNL 147
gi 50401795   95 L--KIFC------TS-----------------KIAKDi-FLEWNWTLW--LTPPE---T---IAKMVWFPLINhhlFESE 140
gi 754415898 102 L--ELRL------NL-----------------VKVYSnaELNWNFDLV--ILPDSsaiT---ILRNLIFHLST---IIFS 148
gi 448083895 101 LgaTDVI------NI-----------------I---FaqDIQWRFELQ--KQSHQiatE---FFASMNIQQFR---NHAF 146
gi 112362763 106 F--SFKV------SA-----------------KLTVSm--nSEPTHVT--HETCF---T---ILQDLSSVLLR---LVNV 147
gi 366992846 110 I--TLNL------TT-----------------IILG----------LS--MQEQL---T---LSENLIRQTLR---IIHI 143
gi 748452793 113 I--NVSI------NS-----------------V-------------SS--QVTKD---McfmILQNVSSVLLQ---LINV 146
gi 207345356 196 L--LDKL-------------------------------------------LETRD---E---RTRAMMVTLLN------E 218
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*...
3W03_A       148 LQCQVRELATLLHMKDLEI-------------------QDYQESGATL 176
gi 74608996  177 ------ELNRIAEAK-----------------------ESNQIYGRDL 195
gi 74644939  148 STQYHYVQRDILNEKQKCLdf---------------llISLRDLDGGS 180
gi 50401795  141 TDTSPGSLMSIIRDQKSLLndktwf--------kealnERSSSSNPST 180
gi 754415898 149 LNEYKNDLIKVVEHKDSAIrf---------------lgESIEGMNGGh 181
gi 448083895 147 LDYKIQQLERAIKDKDHYIly---------------leQNYKAVNGne 179
gi 112362763 148 SAQYQNIQRDVLNEKQKCLdf---------------llRSVNDLDGGk 180
gi 366992846 144 QGLYSNHLLNVITEKDSVIqf---------------lsDSLNDLGASk 176
gi 748452793 147 SAHYQSIQRDIIDRKQKCLdf---------------lvRSVKDLDGGs 179
gi 207345356 219 KKKKIRELHEILRQNNIKLsdddvldsalintevqkpiSELNSPGKRM 266
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