Conserved Protein Domain Family
P53

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cd08367: P53 
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P53 DNA-binding domain
P53 is a tumor suppressor gene product; mutations in p53 or lack of expression are found associated with a large fraction of all human cancers. P53 is activated by DNA damage and acts as a regulator of gene expression that ultimatively blocks progression through the cell cycle. P53 binds to DNA as a tetrameric transcription factor. In its inactive form, p53 is bound to the ring finger protein Mdm2, which promotes its ubiquitinylation and subsequent proteosomal degradation. Phosphorylation of p53 disrupts the Mdm2-p53 complex, while the stable and active p53 binds to regulatory regions of its target genes, such as the cyclin-kinase inhibitor p21, which complexes and inactivates cdk2 and other cyclin complexes.
Links
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BioAssay Targets and Results
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CID: 16031519
AID: 902
GI: 120407068
IC50: 0.0398µM
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qHTS Screen for Compounds that Selectively Target Cancer Cells with p53 Mutations: Cytotoxicity of p53ts Cells at the Nonpermissive Temperature
Chemical Name: MolPort-007-887-326
IC50: 0.0398µM
CID: 14957
AID: 651743
GI: 269849759
IC50: 0.02µM
more
qHTS assay for small molecule activators of the p53 signaling pathway
Target Name: Cellular tumor antigen p53
Chemical Name: Antimycin A
IC50: 0.02µM
CID: 2335783
AID: 624305
GI: 23491729
IC50: 0.0517µM
more
qHTS assay for re-activators of p53 using a Luc reporter: Hit Validation
Chemical Name: AC1M5THE
IC50: 0.0517µM
Statistics
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PSSM-Id: 176262
View PSSM: cd08367
Aligned: 35 rows
Threshold Bit Score: 145.104
Threshold Setting Gi: 229553939
Created: 18-Nov-2009
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:DNA binding site [nucleic acid binding site]
Evidence:

cd08367 is part of a hierarchy of related CD models.
Use the graphical representation to navigate this hierarchy.
cd08367 is a member of the superfamily cl14608.
cd08367:P531T4W A2295539392244857611700329471571672791700329411700329431700329455838495845553461167523367167526646221131137270016435910946152422473572420031021565465322419982321259764012211012051259763991158984542AC0 A2GEQ A28296771293681293748928244269784945747670781930830751183439481960078361043038451T4W A2295539392244857611700329471571672791700329411700329431700329455838495845553461167523367167526646221131137270016435910946152422473572420031021565465322419982321259764012211012051259763991158984542AC0 A2GEQ A2829677129368129374892824426978494574767078193083075118343948196007836104303845
cd08367 Sequence Cluster
cd08367 Sequence Cluster
Sub-family Hierarchy
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CD Hierarchy
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2AC0_A        16 FRLGFLHsgt-aksVTCTYSPa---lNKMFCQl--aKTCPVQLWVdstp---ppgTRVRAMAIYKqsq--------hmtE 78
1T4W_A         4 MEIDVLKqkv-aksSDMAFAIsseheKYLWTKm--gCLVPIQVKWkldkrhfnsnLSLRIRFVKYdkkenveyairnprS 80
gi 170032947 141 FRVNLPSdas--dkTSWMYSDk---lQKLFVKm--gNTCTFNVQLmasp---evtTVVRAMVVCSspe--------dqhH 202
gi 157167279 154 FNVDLNGets--gkSSWMFSSr---lNKVFVKm--gQACTFNISYqalt---hqeLFVRAMMVCSape--------dmhY 215
gi 45553461  203 FSMVLDEpp----kSLWMYSIp---lNKLYIRmnkaFNVDVQFKSkmp----iqpLNLRVFLCFSnd----------vsA 261
gi 241998232  93 FRVVFVKpegvrnsTSWTYSNa---kRKLYTNm--nEFCSVHFITerap---prgSVVCVVGVFLdse--------frrH 156
gi 58384958  102 FTVIPSStq----gSGFIFSEq---lQKLFLKt--dSICSFDIACqlptflpptgWYVRVMLVSLape--------sqhE 164
gi 170032941  74 FKVTLEKsg---snDFHVFSQk---lNRIFTSt--gKSLTFNMAMnph-----tpLLVRIMLVCSnq----------lfQ 130
gi 170032945 128 FKVRIAEtg---rnESYTYSNv---lNKIYTSi--eKHCTFEASFnfcs---tilPIIRVMMVCAna----------pfQ 186
gi 170032943  56 FKVKLDItg---kgDSYEISDa---lDRIYTS----TDRSITFDIvykpe-hgsnLSVRIMLVGSnl----------lhH 114
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2AC0_A        79 VVRRCPHHERCSDsd-------gLAPPq----HLIRVEGNl-RVEYLDdr----nTFRHSVVVPYEppe----vgSDCTT 138
1T4W_A        81 DVMKCRSHTEREQ----------HFPFd----SFFYIRNSehEFSYSA-------EKGSTFTLIMYp-------gAVQAN 132
gi 170032947 203 PVSRCDNHRCSDNq---------DIPDdi-kmHVIRCKNE--QVSYVGtkdgkvfEERLAILVPMEh--------TNRAG 262
gi 157167279 216 PVYRCENHRGSDNvnpk---lpnEVKA-----HVMRCFNP--SARYVGtengvafKDRLAVIIPLGmts----qeQVALN 281
gi 45553461  262 PVVRCQNHLSVEPl---------TANNakmreSLLRSENP--NSVYCGnaqgkgiSERFSVVVPLNmsrsvtrsgLTRQT 330
gi 241998232 157 NVERCTAHARLEDgsnqlpsgkhPFPC-----FWIRCDHP--RTRYCLde---anGGRQCLCLPFEape---aasNDPFT 223
gi 58384958  165 SITRCHKHIAHDTgp-------eEIRK-----HVVRCKNE--QHEYVGadngpffEDRYAVRVPLDd-------eVLCVK 223
gi 170032941 131 PLSRCSYHIRGDNt---------TFRD-----HVVTHRDS--AARYVGtangiyqRERLAILVPIEe--------IGQKS 186
gi 170032945 187 PLSRCAYHTKNDTt---------ANKN-----HVVLRRDG--SAEYIGtpegetfRDRLALKIAMGd--------SCRKN 242
gi 170032943 115 PLARCSYHRKGDNt---------TFKD-----HVVRHKDA--SAEYVGtatgmlyPERLAILVPLDq--------SGLKS 170
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1               # #       #                          # ###  #         
2AC0_A       139 IHYNYMCNSSCmg-gMNRRPILTIITLEDs-sGNLLGRNSF-EVRVCACPGRDRRTEEENL 196
1T4W_A       133 FDIIFMCQEKCldldDRRKTMCLAVFLDDe-nGNEILHAYIkQVRIVAYPRRDWKNFCERE 192
gi 170032947 263 ITLEFVCQNSCr--nINRRATALVFTLEDq-tGRILGRQMF-QIKICTNIKRDKLNEENAV 319
gi 157167279 282 VSLEFVCQNSCr--iINRRTTAIIFTLEDv-hGQILGKKSL-HLKVCSCPKRDKMKEEETV 338
gi 45553461  331 LAFKFVCQNSC----IGRKETSLVFCLEKa-cGDIVGQHVI-HVKICTCPKRDRIQDERQL 385
gi 241998232 224 YHLAFMCRNSCpg-sPGRKATEVVFILQSp-eGRDIARRVI-EVKVCACPCRDRRHDEQAA 281
gi 58384958  224 IMLQFVCQNTCf--rLDQRRTGLVFTLENd-qGNIWARRVV-PVKICINYRRDMQNEQNSA 280
gi 170032941 187 ISLEFKCLSSCy--kIRKIATALVFTLEDensNSVLGRQIF-NVHVSKNYKRDMEVAEKNG 244
gi 170032945 243 ITLEFKCLSSCy--kIKKVSTALVFTLEDpvgGQLLGREII-PIQISKNFKRDMETAEKAA 300
gi 170032943 171 ITLDFVCLNSCh--kIRKLKLALVFTLEDqddKKIVGRQIF-HLQVSKNFKRDMEVAEKGP 228

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Click on image for an interactive view with Cn3D
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D