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1 2 MODULE valid 3 $$ SEQ_INST, 1 4 $^ ExtNotAllowed, 1 5 # This is a comment 6 A Bioseq "extension" is used for special classes of Bioseq. This class of 7 Bioseq should not have one but it does. This is probably a software error. 8 9 $^ ExtBadOrMissing, 2 10 This class of Bioseq requires an "extension" but it is missing or of the 11 wrong type. This is probably a software error. 12 13 $^ SeqDataNotFound, 3 14 No actual sequence data was found on this Bioseq. This is probably a 15 software problem. 16 17 $^ SeqDataNotAllowed, 4 18 The wrong type of sequence data was found on this Bioseq. This is probably a 19 software problem. 20 21 $^ ReprInvalid, 5 22 This Bioseq has an invalid representation class. This is probably a software 23 error. 24 25 $^ CircularProtein, 6 26 This protein Bioseq is represented as circular. Circular topology is 27 normally used only for certain DNA molecules, for example, plasmids. 28 29 $^ DSProtein, 7 30 This protein Bioseq has strandedness indicated. Strandedness is normally a 31 property only of DNA sequences. Please unset the strandedness. 32 33 $^ MolNotSet, 8 34 It is not clear whether this sequence is nucleic acid or protein. Please set 35 the appropriate molecule type (Bioseq.mol). 36 37 $^ MolOther, 9 38 Most sequences are either nucleic acid or protein. However, the molecule 39 type (Bioseq.mol) is set to "other". It should probably be set to nucleic 40 acid or a protein. 41 42 $^ FuzzyLen, 10 43 This sequence is marked as having an uncertain length, but the length is 44 known exactly. 45 46 $^ InvalidLen, 11 47 The length indicated for this sequence is invalid. This is probably a 48 software error. 49 50 $^ InvalidAlphabet, 12 51 This Bioseq has an invalid alphabet (e.g. protein codes on a nucleic acid or 52 vice versa). This is probably a software error. 53 54 $^ SeqDataLenWrong, 13 55 The length of this Bioseq does not agree with the length of the actual data. 56 This is probably a software error. 57 58 $^ SeqPortFail, 14 59 Something is very wrong with this entry. The validator cannot open a SeqPort 60 on the Bioseq. Further testing cannot be done. 61 62 $^ InvalidResidue, 15 63 Invalid residue codes were found in this Bioseq. 64 65 $^ StopInProtein, 16 66 Stop codon symbols were found in this protein Bioseq. 67 68 $^ PartialInconsistent, 17 69 This segmented sequence is described as complete or incomplete in several 70 places, but these settings are inconsistent. 71 72 $^ ShortSeq, 18 73 This Bioseq is unusually short (less than 4 amino acids or less than 11 74 nucleic acids). GenBank does not usually accept such short sequences. 75 76 $^ NoIdOnBioseq, 19 77 No SeqIds were found on this Bioseq. This is probably a software error. 78 79 $^ BadDeltaSeq, 20 80 Delta sequences should only be HTGS-1 or HTGS-2. 81 82 $^ LongHtgsSequence, 21 83 HTGS-1 or HTGS-2 sequences must be < 350 KB in length. 84 85 $^ LongLiteralSequence, 22 86 Delta literals must be < 350 KB in length. 87 88 $^ SequenceExceeds350kbp, 23 89 Individual sequences must be < 350 KB in length, unless they represent a single gene. 90 91 $^ ConflictingIdsOnBioseq, 24 92 Two SeqIds of the same class was found on this Bioseq. This is probably a software error. 93 94 $^ MolNuclAcid, 25 95 The specific type of this nucleic acid (DNA or RNA) is not set. 96 97 $^ ConflictingBiomolTech, 26 98 HTGS/STS/GSS records should be genomic DNA. There is a conflict between the 99 technique and expected molecule type. 100 101 $^ SeqIdNameHasSpace, 27 102 The Seq-id.name field should be a single word without any whitespace. This should be 103 fixed by the database staff. 104 105 $^ IdOnMultipleBioseqs, 28 106 There are multiple occurrences of the same Seq-id in this record. Sequence 107 identifiers must be unique within a record. 108 109 $^ DuplicateSegmentReferences, 29 110 The segmented sequence refers multiple times to the same Seq-id. This may be due 111 to a software error. Please consult with the database staff to fix this record. 112 113 $^ TrailingX, 30 114 The protein sequence ends with one or more X (unknown) amino acids. 115 116 $^ BadSeqIdFormat, 31 117 A nucleotide sequence identifier should be 1 letter plus 5 digits or 2 letters 118 plus 6 digits, and a protein sequence identifer should be 3 letters plus 5 digits. 119 120 $^ PartsOutOfOrder, 32 121 The parts inside a segmented set should correspond to the seq_ext of the segmented 122 bioseq. A difference will affect how the flatfile is displayed. 123 124 $^ BadSecondaryAccn, 33 125 A secondary accession usually indicates a record replaced or subsumed by the current 126 record. In this case, the current accession and secondary are the same. 127 128 $^ ZeroGiNumber, 34 129 GI numbers are assigned to sequences by NCBI's sequence tracking database. 0 is not 130 a legal value for a gi number. 131 132 $^ RnaDnaConflict, 35 133 The MolInfo biomol field is inconsistent with the Bioseq molecule type field. 134 135 $^ HistoryGiCollision, 36 136 The Bioseq history gi refers to this Bioseq, not to its predecessor or successor. 137 138 $^ GiWithoutAccession, 37 139 The Bioseq has a gi identifier but no GenBank/EMBL/DDBJ accession identifier. 140 141 $^ MultipleAccessions, 38 142 The Bioseq has a gi identifier and more than one GenBank/EMBL/DDBJ accession identifier. 143 144 $^ HistAssemblyMissing, 39 145 The Bioseq has a TPA identifier but does not have a Seq-hist.assembly alignment. This 146 should be annotated or calculated by the database, resulting in a PRIMARY block visible 147 in the flatfile. 148 149 $^ TerminalNs, 40 150 The Bioseq has one or more N bases at the end. 151 152 $^ UnexpectedIdentifierChange, 41 153 The set of sequence identifiers on a Bioseq are not consistent with the previous version 154 of the record in the database. 155 156 $^ InternalNsInSeqLit, 42 157 There are runs of many Ns inside the SeqLit component of a delta Bioseq. 158 159 $^ SeqLitGapLength0, 43 160 A SeqLit component of a delta Bioseq can specify a gap, but it should not be a gap 161 of 0 length. 162 163 $^ TpaAssmeblyProblem, 44 164 Third party annotation records should have a TpaAssembly user object and a 165 Seq-hist.assembly alignment for the PRIMARY block. 166 167 $^ SeqLocLength, 45 168 A SeqLoc component of a delta Bioseq is suspiciously small. 169 170 $^ MissingGaps, 46 171 HTGS delta records should have gaps between each sequence segment. 172 173 $^ CompleteTitleProblem, 47 174 The sequence title has complete genome in it, but it is not marked as complete. 175 176 $^ CompleteCircleProblem, 48 177 This sequence has a circular topology, but it is not marked as complete. 178 179 $^ BadHTGSeq, 49 180 High throughput genomic sequences without gaps should have quality score graphs. 181 182 $^ GapInProtein, 50 183 Gap symbols found in this protein Bioseq. 184 185 $^ BadProteinStart, 51 186 A gap symbols was found at the start of this protein Bioseq. 187 188 $^ TerminalGap, 52 189 The Bioseq has a gap at the end. 190 191 $^ OverlappingDeltaRange, 53 192 The Bioseq has a gap at the end. 193 194 $^ LeadingX, 54 195 The protein sequence starts with one or more X (unknown) amino acids. 196 197 $^ InternalNsInSeqRaw, 55 198 There are runs of greater than 100 Ns within sequence. Please describe 199 what these Ns represent with your sequence submission. 200 201 $^ InternalNsAdjacentToGap, 56 202 There are Ns directly adjacent to a SeqLit gap in a delta Bioseq. 203 204 $^ CaseDifferenceInSeqID, 57 205 Multiple Bioseqs have the same Seq-id except for capitalization. Sequence 206 identifiers must be unique in a case-insensitive manner within a record. 207 208 $^ DeltaComponentIsGi0, 58 209 Delta component refers to gi 0. This indicates an error in database processing of this record. 210 211 $^ FarFetchFailure, 59 212 Fetching of a far feature location or component bioseq was needed, but no fetch 213 function is registered in the program. 214 215 $^ InternalGapsInSeqRaw, 60 216 Raw sequences should not have gap characters. 217 218 $^ SelfReferentialSequence, 61 219 A delta sequence must refer to other components, not to itself. 220 221 $^ WholeComponent, 62 222 A delta sequence component should be described as a specific interval, not as 223 the whole of a sequence. 224 225 $^ TSAHistAssemblyMissing, 63 226 The Bioseq has a TSA MolInfo tech but does not have a Seq-hist.assembly alignment. 227 228 $^ ProteinsHaveGeneralID, 64 229 One or more protein Bioseqs have a general Seq-id. 230 231 $^ HighNContent, 65 232 This sequence contains too many Ns. 233 234 $^ SeqLitDataLength0, 66 235 A SeqLit component of a delta Bioseq must not have 0 length. 236 237 $^ DSmRNA, 67 238 This mRNA Bioseq is not single stranded. 239 240 $^ HighNContentStretch, 68 241 This sequence contains long stretches of Ns. 242 243 $^ HighNContentPercent, 69 244 This sequence contains a high percentage of Ns. 245 246 $$ SEQ_DESCR, 2 247 248 $^ BioSourceMissing, 1 249 The biological source of this sequence has not been described correctly. A 250 Bioseq must have a BioSource descriptor that covers the entire molecule. 251 Additional BioSource features may also be added to recombinant molecules, 252 natural or otherwise, to designate the parts of the molecule. Please add the 253 source information. 254 255 $^ InvalidForType, 2 256 This descriptor cannot be used with this Bioseq. A descriptor placed at the 257 BioseqSet level applies to all of the Bioseqs in the set. Please make sure 258 the descriptor is consistent with every sequence to which it applies. 259 260 $^ FileOpenCollision, 3 261 FileOpen is unable to find a local file. This is normal, and can be ignored. 262 263 $^ Unknown, 4 264 An unknown or "other" modifier was used. 265 266 $^ NoPubFound, 5 267 No publications were found in this entry which refer to this Bioseq. If a 268 publication descriptor is added to a BioseqSet, it will apply to all of the 269 Bioseqs in the set. A publication feature should be used if the publication 270 applies only to a subregion of a sequence. 271 272 $^ NoOrgFound, 6 273 This entry does not specify the organism that was the source of the sequence. 274 Please name the organism. 275 276 $^ MultipleBioSources, 7 277 There are multiple BioSource or OrgRef descriptors in the same chain with 278 the same taxonomic name. Their information should be combined into a single 279 BioSource descriptor. 280 281 $^ NoMolInfoFound, 8 282 This sequence does not have a Mol-info descriptor applying to it. This indicates 283 genomic vs. message, sequencing technique, and whether the sequence is incomplete. 284 285 $^ BadCountryCode, 9 286 The country code (up to the first colon) is not on the approved list of countries. 287 288 $^ NoTaxonID, 10 289 The BioSource is missing a taxonID database identifier. This will be inserted by 290 the automated taxonomy lookup called by Clean Up Record. 291 292 $^ InconsistentBioSources, 11 293 This population study has BioSource descriptors with different taxonomic names. 294 All members of a population study should be from the same organism. 295 296 $^ MissingLineage, 12 297 A BioSource should have a taxonomic lineage, which can be obtained from the 298 taxonomy network server. 299 300 $^ SerialInComment, 13 301 Comments that refer to the conclusions of a specific reference should not be 302 cited by a serial number inside brackets (e.g., [3]), but should instead be 303 attached as a REMARK on the reference itself. 304 305 $^ BioSourceNeedsFocus, 14 306 Focus must be set on a BioSource descriptor in records where there is a 307 BioSource feature with a different organism name. 308 309 $^ BadOrganelle, 15 310 Note that only Kinetoplastida have kinetoplasts, and that only Chlorarchniophyta 311 and Cryptophyta have nucleomorphs. 312 313 $^ MultipleChromosomes, 16 314 There are multiple chromosome qualifiers on this Bioseq. With the exception of 315 some pseudoautosomal genes, this is likely to be a biological annotation error. 316 317 $^ BadSubSource, 17 318 Unassigned SubSource subtype. 319 320 $^ BadOrgMod, 18 321 Unassigned OrgMod subtype. 322 323 $^ InconsistentProteinTitle, 19 324 An instantiated protein title descriptor should normally be the same as the 325 automatically generated title. This may be a curated exception, or it may 326 be out of synch with the current annotation. 327 328 $^ Inconsistent, 20 329 There are two descriptors of the same type which are inconsistent with each 330 other. Please make them consistent. 331 332 $^ ObsoleteSourceLocation, 21 333 There is a source location that is no longer legal for use in GenBank records. 334 335 $^ ObsoleteSourceQual, 22 336 There is a source qualifier that is no longer legal for use in GenBank records. 337 338 $^ StructuredSourceNote, 23 339 The name of a structured source field is present as text in a note. The data 340 should probably be put into the appropriate field instead. 341 342 $^ UnnecessaryBioSourceFocus, 24 343 Focus should not be set on a BioSource descriptor in records where there is no 344 BioSource feature. 345 346 $^ RefGeneTrackingWithoutStatus, 25 347 The RefGeneTracking user object does not have the required Status field set. 348 349 $^ UnwantedCompleteFlag, 26 350 The Mol-info.completeness flag should not be set on a genomic sequence unless 351 the title also says it is a complete sequence or complete genome. 352 353 $^ CollidingPublications, 27 354 Multiple publication descriptors with the same PMID or MUID apply to a Bioseq. 355 The lower-level ones are redundant, and should be removed. 356 357 $^ TransgenicProblem, 28 358 A BioSource descriptor with /transgenic set must be accompanied by a BioSource 359 feature on the nucleotide record. 360 361 $^ TaxonomyLookupProblem, 29 362 A BioSource descriptor or feature has flags returned by taxonomy lookup that 363 are either inconsistent with the data or require a taxonomy consult. 364 365 $^ MultipleTitles, 30 366 There are multiple title descriptors in the same Bioseq or BioseqSet chain. 367 368 $^ RefGeneTrackingOnNonRefSeq, 31 369 The RefGeneTracking user object should only be in RefSeq records. 370 371 $^ BioSourceInconsistency, 32 372 There is an internal inconsistency with specific fields in the BioSource. 373 374 $^ FastaBracketTitle, 33 375 Bracketed [...=...] information remains in the title. This should have been parsed 376 out during sequence record generation to obtain qualifier values. 377 378 $^ MissingText, 34 379 Comments, regions, and other text descriptors need a descriptive text string. 380 The string provided with this descriptor is empty. If no text is desired, then 381 the descriptor should be removed. 382 383 $^ BadCollectionDate, 35 384 The collection date is not in the required format. 385 386 $^ BadPCRPrimerSequence, 36 387 The PCR primer sequence has illegal characters or non-IUPAC nucleotides. 388 389 $^ BadPunctuation, 37 390 The title ends with incorrect punctuation marks. 391 392 $^ BadPCRPrimerName, 38 393 The PCR primer name appears to be a sequence instead of an identifying label. 394 395 $^ BioSourceOnProtein, 39 396 A BioSource descriptor should not be placed on a protein that is in a nuc-prot set. 397 398 $^ BioSourceDbTagConflict, 40 399 Multiple db_xrefs with the same database should not appear on a single BioSource. 400 401 $^ DuplicatePCRPrimerSequence, 41 402 The PCR primer sequence has duplicate subsequences. 403 404 $^ MultipleNames, 42 405 There are multiple name descriptors in the same Bioseq or BioseqSet chain. 406 407 $^ MultipleComments, 43 408 There are multiple identical comment descriptors in the same Bioseq or BioseqSet chain. 409 410 $^ LatLonProblem, 44 411 There is a problem with the lat_lon qualifier in the BioSource. 412 413 $^ LatLonFormat, 45 414 The format of lat_lon should be dd.dd N|S ddd.dd E|W. 415 416 $^ LatLonRange, 46 417 Latitude or longitude is out of range. 418 419 $^ LatLonValue, 47 420 Latitude or longitude values appear to be in the wrong hemisphere or swapped. 421 422 $^ LatLonCountry, 48 423 The lat_lon coordinate does not map to the indicated country. 424 425 $^ LatLonState, 49 426 The lat_lon coordinate does not map to the indicated state or province. 427 428 $^ BadSpecificHost, 50 429 A BioSource descriptor or feature has a specific host value that may 430 require a taxonomy consult. 431 432 $^ RefGeneTrackingIllegalStatus, 51 433 The RefGeneTracking user object has an illegal Status value. 434 435 $^ ReplacedCountryCode, 52 436 The country code (up to the first colon) is no longer on the approved list of countries. 437 438 $^ BadInstitutionCode, 53 439 The institution (or institution: collection) code is not on the approved list. 440 441 $^ BadCollectionCode, 54 442 The institution code is recognized, but the collection is not on the approved list. 443 444 $^ BadVoucherID, 55 445 The voucher is missing a specific identifier. 446 447 $^ UnstructuredVoucher, 56 448 The voucher needs to be structured as "<institution-code>:[<collection-code>:]<culture_id>". 449 450 $^ ChromosomeLocation, 57 451 BioSource location is not usually chromosome. 452 453 $^ MultipleSourceQualifiers, 58 454 BioSource has unexpected multiple qualifiers of the same type. 455 456 $^ UnbalancedParentheses, 59 457 Qualifier should have matching ( and ) parentheses or [ and ] brackets. 458 459 $^ MultipleSourceVouchers, 60 460 BioSource has unexpected multiple bio_material/culture_collection/specimen_voucher 461 from the same institution. 462 463 $^ BadCountryCapitalization, 61 464 The country code does not use the correct capitalization. 465 466 $^ WrongVoucherType, 62 467 The institution (or institution: collection) code normally uses a different 468 bio_material/culture_collection/specimen_voucher type. 469 470 $^ UserObjectProblem, 63 471 The user object is missing required fields or has invalid data. 472 473 $^ TitleHasPMID, 64 474 The title has (PMID #####) embedded in it. 475 476 $^ BadKeyword, 65 477 The keyword is not appropriate in this record. 478 479 $^ NoOrganismInTitle, 66 480 A RefSeq record should have the organism name at the beginning of a nucleotide 481 title and bracketed at the end of a protein title. 482 483 $^ MissingChromosome, 67 484 An NC or AC RefSeq record should have a chromosome annotated. 485 486 $^ LatLonAdjacent, 68 487 The lat_lon coordinate may be in an adjacent country or in surrounding waters. 488 489 $^ BadStructuredCommentFormat, 69 490 Structured comment is missing required fields or field values do not conform to correct format. 491 492 493 $$ GENERIC, 3 494 495 $^ NonAsciiAsn, 1 496 There is a non-ASCII type character in this entry. 497 498 $^ Spell, 2 499 There is a potentially misspelled word in this entry. 500 501 $^ AuthorListHasEtAl, 3 502 The author list contains et al, which should be replaced with the 503 remaining author names. 504 505 $^ MissingPubInfo, 4 506 The publication is missing essential information, such as title or authors. 507 508 $^ UnnecessaryPubEquiv, 5 509 A nested Pub-equiv is not normally expected in a publication. This may prevent 510 proper display of all publication information. 511 512 $^ BadPageNumbering, 6 513 The publication page numbering is suspect. 514 515 $^ MedlineEntryPub, 7 516 Publications should not be of type medline-entry. This has abstract and MeSH 517 term information that does not appear in the GenBank flatfile. Type cit-art 518 should be used instead. 519 520 $^ BadDate, 8 521 There are bad values for month, day, or year in a date. 522 523 $^ StructuredCitGenCit, 9 524 The publication has title or journal embedded in the unstructured citgen.cit 525 field. 526 527 $^ CollidingSerialNumbers, 10 528 Multiple publications have the same serial number explicitly recorded in the 529 data. 530 531 $^ EmbeddedScript, 11 532 Script or other markup tags should not be used in sequence record fields. 533 534 535 $^ PublicationInconsistency, 12 536 Some fields in the publication should not be present with other fields. 537 538 $^ SgmlPresentInText, 13 539 SGML markup is embedded in text. 540 541 $^ UnexpectedPubStatusComment, 14 542 An unexpected publication status exists for a print, online-only, 543 or ahead-of-print article : Content-Of-Pubdesc.comment-String. 544 545 $$ SEQ_PKG, 4 546 547 $^ NoCdRegionPtr, 1 548 A protein is found in this entry, but the coding region has not been 549 described. Please add a CdRegion feature to the nucleotide Bioseq. 550 551 $^ NucProtProblem, 2 552 Both DNA and protein sequences were expected, but one of the two seems to be 553 missing. Perhaps this is the wrong package to use. 554 555 $^ SegSetProblem, 3 556 A segmented sequence was expected, but it was not found. Perhaps this is the 557 wrong package to use. 558 559 $^ EmptySet, 4 560 No Bioseqs were found in this BioseqSet. Is that what was intended? 561 562 $^ NucProtNotSegSet, 5 563 A nuc-prot set should not contain any other BioseqSet except segset. 564 565 $^ SegSetNotParts, 6 566 A segset should not contain any other BioseqSet except parts. 567 568 $^ SegSetMixedBioseqs, 7 569 A segset should not contain both nucleotide and protein Bioseqs. 570 571 $^ PartsSetMixedBioseqs, 8 572 A parts set should not contain both nucleotide and protein Bioseqs. 573 574 $^ PartsSetHasSets, 9 575 A parts set should not contain BioseqSets. 576 577 $^ FeaturePackagingProblem, 10 578 A feature should be packaged on its bioseq, or on a set containing the Bioseq. 579 580 $^ GenomicProductPackagingProblem, 11 581 The product of an mRNA feature in a genomic product set should point to a cDNA 582 Bioseq packaged in the set, perhaps within a nuc-prot set. RefSeq records may 583 however be referenced remotely. 584 585 $^ InconsistentMolInfoBiomols, 12 586 Mol-info.biomol is inconsistent within a segset, parts set, or pop/phy/mut/eco set. 587 588 $^ ArchaicFeatureLocation, 13 589 A feature location should refer to the accession or gi number, not a local or general ID. 590 591 $^ ArchaicFeatureProduct, 14 592 A feature product should refer to the accession or gi number, not a local or general ID. 593 594 $^ GraphPackagingProblem, 15 595 A graph should be packaged on its bioseq, or on a set containing the Bioseq. 596 597 $^ InternalGenBankSet, 16 598 An outer BioseqSet should not contain an internal BioseqSet of class genbank. 599 600 $^ ConSetProblem, 17 601 BioseqSet class should not be conset. 602 603 $^ NoBioseqFound, 18 604 No Bioseqs were found in the entire record. 605 606 $^ INSDRefSeqPackaging, 19 607 INSD and RefSeq records should not be packaged together. 608 609 $^ GPSnonGPSPackaging, 20 610 Genomic product set records should not be packaged with other set types. 611 612 $^ RefSeqPopSet, 21 613 RefSeq records should not be packaged in a popset. 614 615 $^ BioseqSetClassNotSet, 22 616 The BioseqSet class field is not set. 617 618 $^ OrphanedProtein, 23 619 The Bioseq is an INSD or RefSeq protein erroneously not in a nuc-prot set. 620 621 622 $$ SEQ_FEAT, 5 623 624 $^ InvalidForType, 1 625 This feature type is illegal on this type of Bioseq. 626 627 $^ PartialProblem, 2 628 There are several places in an entry where a sequence can be described as 629 either partial or complete. In this entry, these settings are inconsistent. 630 Make sure that the location and product Seq-locs, the Bioseqs, and the 631 SeqFeat partial flag all agree in describing this SeqFeat as partial or 632 complete. 633 634 $^ InvalidType, 3 635 A feature with an invalid type has been detected. This is most likely a 636 software problem. 637 638 $^ Range, 4 639 The coordinates describing the location of a feature do not fall within the 640 sequence itself. A feature location or a product Seq-loc is out of range of 641 the Bioseq it points to. 642 643 $^ MixedStrand, 5 644 Mixed strands (plus and minus) have been found in the same location. While 645 this is biologically possible, it is very unusual. Please check that this is 646 really what you mean. 647 648 $^ SeqLocOrder, 6 649 This location has intervals that are out of order. While whis is biologically 650 possible, it is very unusual. Please check that this is really what you mean. 651 652 $^ CdTransFail, 7 653 A fundamental error occurred in software while attempting to translate this 654 coding region. It is either a software problem or sever data corruption. 655 656 $^ StartCodon, 8 657 An illegal start codon was used. Some possible explanations are: (1) the 658 wrong genetic code may have been selected; (2) the wrong reading frame may 659 be in use; or (3) the coding region may be incomplete at the 5' end, in 660 which case a partial location should be indicated. 661 662 $^ InternalStop, 9 663 Internal stop codons are found in the protein sequence. Some possible 664 explanations are: (1) the wrong genetic code may have been selected; (2) the 665 wrong reading frame may be in use; (3) the coding region may be incomplete 666 at the 5' end, in which case a partial location should be indicated; or (4) 667 the CdRegion feature location is incorrect. 668 669 $^ NoProtein, 10 670 Normally a protein sequence is supplied. This sequence can then be compared 671 with the translation of the coding region. In this entry, no protein Bioseq 672 was found, and the comparison could not be made. 673 674 $^ MisMatchAA, 11 675 The protein sequence that was supplied is not identical to the translation 676 of the coding region. Mismatching amino acids are found between these two 677 sequences. 678 679 $^ TransLen, 12 680 The protein sequence that was supplied is not the same length as the 681 translation of the coding region. Please determine why they are different. 682 683 $^ NoStop, 13 684 A coding region that is complete should have a stop codon at the 3'end. A 685 stop codon was not found on this sequence, although one was expected. 686 687 $^ TranslExcept, 14 688 An unparsed \transl_except qualifier was found. This indicates a parser 689 problem. 690 691 $^ NoProtRefFound, 15 692 The name and description of the protein is missing from this entry. Every 693 protein Bioseq must have one full-length Prot-ref feature to provide this 694 information. 695 696 $^ NotSpliceConsensus, 16 697 Splice junctions typically have GT as the first two bases of the intron 698 (splice donor) and AG as the last two bases of the intron (splice acceptor). 699 This intron does not conform to that pattern. 700 701 $^ OrfCdsHasProduct, 17 702 A coding region flagged as orf has a protein product. There should be no 703 protein product bioseq on an orf. 704 705 $^ GeneRefHasNoData, 18 706 A gene feature exists with no locus name or other fields filled in. 707 708 $^ ExceptInconsistent, 19 709 A coding region has an exception gbqual but the excpt flag is not set. 710 711 $^ ProtRefHasNoData, 20 712 A protein feature exists with no name or other fields filled in. 713 714 $^ GenCodeMismatch, 21 715 The genetic code stored in the BioSource is different than that for this CDS. 716 717 $^ RNAtype0, 22 718 RNA type 0 (unknown RNA) should be type 255 (other). 719 720 $^ UnknownImpFeatKey, 23 721 An import feature has an unrecognized key. 722 723 $^ UnknownImpFeatQual, 24 724 An import feature has an unrecognized qualifier. 725 726 $^ WrongQualOnImpFeat, 25 727 This qualifier is not legal for this feature. 728 729 $^ MissingQualOnImpFeat, 26 730 An essential qualifier for this feature is missing. 731 732 $^ PseudoCdsHasProduct, 27 733 A coding region flagged as pseudo has a protein product. There should be no 734 protein product bioseq on a pseudo CDS. 735 736 $^ IllegalDbXref, 28 737 The database in a cross-reference is not on the list of officially 738 recognized database abbreviations. 739 740 $^ FarLocation, 29 741 The location has a reference to a bioseq that is not packaged in this record. 742 743 $^ DuplicateFeat, 30 744 The intervals on this feature are identical to another feature of the same type, 745 but the label or comment are different. 746 747 $^ UnnecessaryGeneXref, 31 748 This feature has a gene xref that is identical to the overlapping gene. This is 749 redundant, and probably should be removed. 750 751 $^ TranslExceptPhase, 32 752 A \transl_except qualifier was not on a codon boundary. 753 754 $^ TrnaCodonWrong, 33 755 The tRNA codon recognized does not code for the indicated amino acid using the 756 specified genetic code. 757 758 $^ BothStrands, 34 759 Feature location indicates that it is on both strands. This is not biologically 760 possible for this kind of feature. Please indicate the correct strand (plus or 761 minus) for this feature. 762 763 $^ CDSgeneRange, 35 764 A CDS is overlapped by a gene feature, but is not completely contained by it. 765 This may be an annotation error. 766 767 $^ CDSmRNArange, 36 768 A CDS is overlapped by an mRNA feature, but the mRNA does not cover all 769 intervals (i.e., exons) on the CDS. This may be an annotation error. 770 771 $^ OverlappingPeptideFeat, 37 772 The intervals on this processed protein feature overlap another protein feature. 773 This may be caused by errors in originally annotating these features on DNA coordinates, 774 where start or stop positions do not occur in between codon boundaries. These then 775 appear as errors when the features are converted to protein coordinates by mapping 776 through the CDS. 777 778 $^ SerialInComment, 38 779 Comments that refer to the conclusions of a specific reference should not be 780 cited by a serial number inside brackets (e.g., [3]), but should instead be 781 attached as a REMARK on the reference itself. 782 783 $^ MultipleCDSproducts, 39 784 More than one CDS feature points to the same protein product. This can happen with 785 viral long terminal repeats (LTRs), but GenBank policy is to have each equivalent 786 CDS point to a separately accessioned protein Bioseq. 787 788 $^ FocusOnBioSourceFeature, 40 789 The /focus flag is only appropriate on BioSource descriptors, not BioSource features. 790 791 $^ PeptideFeatOutOfFrame, 41 792 The start or stop positions of this processed peptide feature do not occur in between 793 codon boundaries. This may incorrectly overlap other peptides when the features are 794 converted to protein coordinates by mapping through the CDS. 795 796 $^ InvalidQualifierValue, 42 797 The value of this qualifier is constrained to a particular vocabulary of style. This 798 value does not conform to those constraints. Please see the feature table documentation 799 for more information. 800 801 $^ MultipleMRNAproducts, 43 802 More than one mRNA feature points to the same cDNA product. This is an error in the 803 genomic product set. Each mRNA feature should have a unique product Bioseq. 804 805 $^ mRNAgeneRange, 44 806 An mRNA is overlapped by a gene feature, but is not completely contained by it. 807 This may be an annotation error. 808 809 $^ TranscriptLen, 45 810 The mRNA sequence that was supplied is not the same length as the 811 transcription of the mRNA feature. Please determine why they are different. 812 813 $^ TranscriptMismatches, 46 814 The mRNA sequence and the transcription of the mRNA feature are different. 815 If the number is large, it may indicate incorrect intron/exon boundaries. 816 817 $^ CDSproductPackagingProblem, 47 818 The nucleotide location and protein product of the CDS are not packaged together 819 in the same nuc-prot set. This may be an error in the software used to create 820 the record. 821 822 $^ DuplicateInterval, 48 823 The location has identical adjacent intervals, e.g., a duplicate exon reference. 824 825 $^ PolyAsiteNotPoint, 49 826 A polyA_site should be at a single nucleotide position. 827 828 $^ ImpFeatBadLoc, 50 829 An import feature loc field does not equal the feature location. This should be 830 corrected, and then the loc field should be cleared. 831 832 $^ LocOnSegmentedBioseq, 51 833 Feature locations traditionally go on the individual parts of a segmented bioseq, not 834 on the segmented sequence itself. These features are invisible in asn2ff reports, and 835 are now being flagged for correction. 836 837 $^ UnnecessaryCitPubEquiv, 52 838 A set of citations on a feature should not normally have a nested Pub-equiv construct. 839 This may prevent proper matching to the correct publication. 840 841 $^ ImpCDShasTranslation, 53 842 A CDS that has known translation errors cannot have a /translation qualifier. 843 844 $^ ImpCDSnotPseudo, 54 845 A CDS that has known translation errors must be marked as pseudo to suppress the 846 translation. 847 848 $^ MissingMRNAproduct, 55 849 The mRNA feature points to a cDNA product that is not packaged in the record. 850 This is an error in the genomic product set. 851 852 $^ AbuttingIntervals, 56 853 The start of one interval is next to the stop of another. A single interval 854 may be desirable in this case. 855 856 $^ CollidingGeneNames, 57 857 Two gene features should not have the same name. 858 859 $^ MultiIntervalGene, 58 860 A gene feature on a single Bioseq should have a single interval spanning everything 861 considered to be under that gene. 862 863 $^ FeatContentDup, 59 864 The intervals on this feature are identical to another feature of the same type, 865 and the label and comment are also identical. This is likely to be an error in 866 annotating the record. Note that GenBank format suppresses duplicate features, 867 so use of Graphic view is recommended. 868 869 $^ BadProductSeqId, 60 870 The feature product refers to a database ID that has a locus name but no accession. 871 This is probably an error in parsing of a submission. 872 873 $^ RnaProductMismatch, 61 874 The RNA feature product type does not correspond to the RNA feature type. These 875 need to be consistent. 876 877 $^ MissingCDSproduct, 62 878 The CDS should have a product, but does not. Pseudo or short CDSs (less than 6 879 amino acids), or those marked with a rearrangement required for product exception, 880 are exempt from needing a product. 881 882 $^ BadTrnaCodon, 63 883 The tRNA codon recognized is an illegal value. 884 885 $^ BadTrnaAA, 64 886 The tRNA encoded amino acid is an illegal value. 887 888 $^ OnlyGeneXrefs, 65 889 There are gene xrefs but no gene features. Records should normally have single-interval 890 gene features covering other biological features. Gene xrefs are used only to override 891 the inheritance by overlap. 892 893 $^ UTRdoesNotAbutCDS, 66 894 The 5'UTR and 3'UTR features should exactly abut the CDS feature. 895 896 $^ BadConflictFlag, 67 897 The coding region conflict flag is set, but the translated product is the 898 same as the instantiated product Bioseq. 899 900 $^ ConflictFlagSet, 68 901 The coding region conflict flag is appropriately set, but this record should 902 be brought to the attention of the source database for possible correction. 903 904 $^ LocusTagProblem, 69 905 A gene locus_tag should be a single token, with no spaces. 906 907 $^ CollidingLocusTags, 70 908 Two gene features should not have the same locus_tag, which is supposed to be 909 a unique identifer. 910 911 $^ AltStartCodon, 71 912 An alternative start codon was used. This is rare, and it is expected that 913 confirmatory evidence will be cited. 914 915 $^ PartialsInconsistent, 72 916 There are several places in an entry where a sequence can be described as 917 either partial or complete. In this entry, these settings are inconsistent. 918 Make sure that the location and product Seq-locs, the Bioseqs, and the 919 SeqFeat partial flag all agree in describing this SeqFeat as partial or 920 complete. 921 922 $^ GenesInconsistent, 73 923 The gene on the genomic sequence of a genomic product set should be the 924 same as the gene on the cDNA product of the mRNA feature. 925 926 $^ DuplicateTranslExcept, 74 927 There are multiple /transl_except qualifiers at the same location on this 928 CDS but with different amino acids indicated. 929 930 $^ TranslExceptAndRnaEditing, 75 931 A CDS has both /exception=RNA editing and /transl_except qualifiers. RNA 932 editing indicates post-transcriptional changes prior to translation. Use 933 /transl_except for individual codon exceptions such as selenocysteine or 934 other nonsense suppressors. 935 936 $^ NoNameForProtein, 76 937 A protein feature has a description, but no product name. 938 939 $^ TaxonDbxrefOnFeature, 77 940 A BioSource feature has a taxonID database identifier in the db_xref area 941 common to all features. This db_xref should only exist within the separate 942 BioSource xref list. 943 944 $^ UnindexedFeature, 78 945 The location of a feature does not allow it to be mapped to a single Bioseq, 946 or to the segmented parent if on one or more part Bioseqs. It will not show 947 up in flatfile and other formats, and should be corrected or removed. 948 949 $^ CDSmRNAmismatch, 79 950 There should usually be a one-to-one correspondence between mRNA and CDS 951 under a given gene. 952 953 $^ UnnecessaryException, 80 954 The feature is marked with an exception qualifier, but the validator does 955 not detect an error that needs to be suppressed. 956 957 $^ LocusTagProductMismatch, 81 958 In certain records a policy is that the locus_tag of the gene is expected to 959 match the prefix of the general ID of the CDS or mRNA product Bioseq. 960 961 $^ MrnaTransFail, 82 962 A fundamental error occurred in software while attempting to transcribe this 963 messenger RNA. It is either a software problem or sever data corruption. 964 965 $^ PseudoCdsViaGeneHasProduct, 83 966 A coding region overlapped by a pseudo gene has a protein product. There 967 should be no protein product bioseq on a pseudo CDS. 968 969 $^ MissingGeneXref, 84 970 This feature has multiple overlapping genes of the same length, but no 971 xref to determine which one should be used for the /gene qualifier. 972 973 $^ FeatureCitationProblem, 85 974 This feature has a citation to a publication that needs to be repaired. 975 976 $^ NestedSeqLocMix, 86 977 A location should not have nested SEQLOC_MIX structures. 978 979 $^ WrongQualOnFeature, 87 980 This qualifier is not legal for this feature. 981 982 $^ MissingQualOnFeature, 88 983 An essential qualifier for this feature is missing. 984 985 $^ CodonQualifierUsed, 89 986 The codon qualifier should be replaced by individual transl_except code breaks, 987 after checking to make sure the proper genetic code is being used. 988 989 $^ UnknownFeatureQual, 90 990 A feature has an unrecognized qualifier. 991 992 $^ BadCharInAuthorName, 91 993 An author name has illegal characters. 994 995 $^ PolyATail, 92 996 The mRNA feature has polyA tail added to make the mRNA sequence. 997 998 $^ ProteinNameEndsInBracket, 93 999 The protein name ends with a right square bracket, and may have been 1000 copied from another GenPept record, where the organism name is 1001 appended to the definition line and placed inside brackets. 1002 1003 $^ CDSwithMultipleMRNAs, 94 1004 The CDS feature has more than one overlapping mRNA with the proper intervals and 1005 no other identification assigning it to a different coding region. 1006 1007 $^ MultipleEquivBioSources, 95 1008 Multiple equivalent biosource features exist on the sequence. They should be 1009 fused into a single feature with multiple intervals. 1010 1011 $^ MultipleEquivPublications, 96 1012 Multiple equivalent publication features exist on the sequence. They should be 1013 fused into a single feature with multiple intervals. 1014 1015 $^ BadFullLengthFeature, 97 1016 A publication or biosource feature is on the full length the sequence. It should be 1017 converted to a publication or biosource descriptor. 1018 1019 $^ RedundantFields, 98 1020 A comment or other field contains information that is redundant with the primary 1021 field on a feature. 1022 1023 $^ CDSwithNoMRNAOverlap, 99 1024 The CDS feature has more than one overlapping mRNA with the proper intervals and 1025 no other identification assigning it to a different coding region. 1026 1027 $^ FeatureProductInconsistency, 100 1028 The CDS feature has more than one overlapping mRNA with the proper intervals and 1029 no other identification assigning it to a different coding region. 1030 1031 $^ ImproperBondLocation, 101 1032 Only bond features should have locations of type bond. Most other features should 1033 use interval or point locations. 1034 1035 $^ GeneXrefWithoutGene, 102 1036 This feature has a gene xref, but there is no equivalent gene feature anywhere 1037 on the record. 1038 1039 $^ SeqFeatXrefProblem, 103 1040 This feature has a seqfeat xref, but it has nothing in the id or data field. 1041 1042 $^ ProductFetchFailure, 104 1043 Unable to fetch the Bioseq product of this feature. Remote fetching was requested, 1044 so this is likely due to a failure in a network sequence retrieval service. 1045 1046 $^ SuspiciousGeneXref, 105 1047 This feature has a gene xref that is not expected for this organism. 1048 1049 $^ MissingTrnaAA, 106 1050 The tRNA encoded amino acid is not set. 1051 1052 $^ CollidingFeatureIDs, 107 1053 Multiple features have the same featureID. Feature ID must be unique within 1054 a record. 1055 1056 $^ ExceptionProblem, 108 1057 There is a problem with /exception text. 1058 1059 $^ PolyAsignalNotRange, 109 1060 A polyA_signal should be at nucleotide range position, not a single point. 1061 1062 $^ OldLocusTagMismtach, 110 1063 The old_locus_tag qualifier on a feature does not match that on the overlapping gene. 1064 1065 $^ DuplicateGeneOntologyTerm, 111 1066 A feature has multiple identical Gene Ontology (GO) term specifications. 1067 1068 $^ InvalidInferenceValue, 112 1069 The value of the inference qualifier is constrained by agreement of the international 1070 nucleotide sequence database collaboration. This value does not conform to those 1071 constraints. Please see the feature table documentation for more information. 1072 1073 $^ HpotheticalProteinMismatch, 113 1074 There is a mismatch between the accession cited by the hypothetical protein claim 1075 and the actual accession of the record. 1076 1077 $^ FeatureRefersToAccession, 114 1078 There is a mixture of features referring to sequence by gi numbers and by accession. 1079 This inconsistency is likely due to incomplete processing by software. 1080 1081 $^ SelfReferentialProduct, 115 1082 A feature product points to the same sequence that the feature location does. 1083 The product must point to a different sequence that is the biological product 1084 of the first, due to transcription, translation, or peptide processing. 1085 1086 $^ ITSdoesNotAbutRRNA, 116 1087 The internal transcribed spacer misc_RNA features should exactly abut the flanking rRNA features. 1088 1089 $^ FeatureSeqIDCaseDifference, 117 1090 Feature location and referenced Bioseq have the same Seq-id except for capitalization. 1091 Sequence identifiers must be unique in a case-insensitive manner within a record. 1092 1093 $^ FeatureLocationIsGi0, 118 1094 Feature location refers to gi 0. This indicates an error in database processing of this record. 1095 1096 $^ GapFeatureProblem, 119 1097 Gap features must only cover gaps in the sequence, not actual bases. 1098 1099 $^ PseudoCdsHasProtXref, 120 1100 A coding region flagged as pseudo has a protein cross reference. There should be no 1101 protein product bioseq or protein cross reference on a pseudo CDS. 1102 1103 $^ ErroneousException, 121 1104 The feature is marked with a specific exception qualifier, but validation indicates 1105 that a different exception should be used. 1106 1107 $^ SegmentedGeneProblem, 122 1108 A gene feature on the parts of a segmented Bioseq should map to a single interval on the 1109 segmented Bioseq's coordinate system. 1110 1111 $^ WholeLocation, 123 1112 Feature location is set to whole, the entirety of the parent sequence. Please specify 1113 exact intervals for this feature location. 1114 1115 $^ BadEcNumberFormat, 124 1116 There is a problem with the format of the EC_number qualifier. 1117 1118 $^ BadEcNumberValue, 125 1119 There is a problem with the value of the EC_number qualifier. 1120 1121 $^ EcNumberProblem, 126 1122 There is a problem with the format of the EC_number qualifier. 1123 1124 $^ VectorContamination, 127 1125 Contamination by cloning vector is annotated on this sequence. The underlying region 1126 should be removed from the sequence. 1127 1128 $^ MinusStrandProtein, 128 1129 A feature on a protein indicates the minus strand, which does not exist. 1130 1131 $^ BadProteinName, 129 1132 A protein feature has a name that conflicts with other information on the feature. 1133 1134 $^ GeneXrefWithoutLocus, 130 1135 A feature has a gene xref with a locus_tag and no locus, but the gene with that locus_tag has a locus. 1136 1137 $^ UTRdoesNotExtendToEnd, 131 1138 The UTR does not have the expected range. 1139 1140 $^ CDShasTooManyXs, 132 1141 The CDS translation has greater than 50 percent ambiguous X residues. 1142 1143 $^ SuspiciousFrame, 133 1144 The CDS has a frame greater than 1 that is not expected in this context. 1145 1146 $^ TerminalXDiscrepancy, 134 1147 The CDS translation and protein product sequence have a different number of terminal Xs. 1148 1149 $^ UnnecessaryTranslExcept, 135 1150 The CDS translates to the same amino acid as indicated by the transl_except code break. 1151 1152 $^ SuspiciousQualifierValue, 136 1153 The value of this qualifier is constrained to a particular vocabulary of style. This 1154 value appears not to conform to those constraints. Please see the feature table documentation 1155 for more information. 1156 1157 $^ NotSpliceConsensusDonor, 137 1158 Splice junctions typically have GT as the first two bases of the intron 1159 (splice donor) and AG as the last two bases of the intron (splice acceptor). 1160 This intron does not conform to that pattern. 1161 1162 $^ NotSpliceConsensusAcceptor, 138 1163 Splice junctions typically have GT as the first two bases of the intron 1164 (splice donor) and AG as the last two bases of the intron (splice acceptor). 1165 This intron does not conform to that pattern. 1166 1167 $^ RareSpliceConsensusDonor, 139 1168 Splice junctions typically have GT as the first two bases of the intron 1169 (splice donor) and AG as the last two bases of the intron (splice acceptor). 1170 This intron does not conform to that pattern. 1171 1172 $^ SeqFeatXrefNotReciprocal, 140 1173 Feature xrefs between CDS and mRNA are not reciprocal. 1174 1175 $^ SeqFeatXrefFeatureMissing, 141 1176 The feature referenced by a seqfeat xref cannot be found. 1177 1178 $^ FeatureInsideGap, 142 1179 The feature is completely contained inside a sequence gap. 1180 1181 $^ FeatureCrossesGap, 143 1182 The feature extends from actual sequence into a sequence gap. 1183 1184 $^ BadAuthorSuffix, 144 1185 An author name has an unexpected suffix. 1186 1187 $^ BadAnticodonAA, 145 1188 The tRNA encoded amino acid cannot be produced by any likely reverse complement 1189 and wobble expansion of the anticodon sequence. 1190 1191 $^ BadAnticodonCodon, 146 1192 The tRNA indicated codon recognized cannot be produced by any likely reverse complement 1193 and wobble expansion of the anticodon sequence. 1194 1195 $^ BadAnticodonStrand, 147 1196 The tRNA feature location and anticodon location are not on the same strand. 1197 1198 $^ UndesiredGeneSynonym, 148 1199 The gene synonym is uninformative. 1200 1201 $^ UndesiredProteinName, 149 1202 The protein name is uninformative. 1203 1204 $^ FeatureBeginsOrEndsInGap, 150 1205 The feature starts or stops within a sequence gap. 1206 1207 $^ GeneOntologyTermMissingGOID, 151 1208 A Gene Ontology (GO) term is missing the GO Identifier. 1209 1210 $^ PseudoRnaHasProduct, 152 1211 An RNA flagged as pseudo has a transcribed product. There should be no 1212 transcribed product bioseq on a pseudo RNA. 1213 1214 $^ PseudoRnaViaGeneHasProduct, 153 1215 An RNA overlapped by a pseudo gene has a transcribed product. There 1216 should be no transcribed product bioseq on a pseudo RNA. 1217 1218 $^ BadRRNAcomponentOrder, 154 1219 rRNA and ITS features are not in the expected order. 1220 1221 $^ BadRRNAcomponentOverlap, 155 1222 rRNA and ITS or tRNA elements should not overlap. 1223 1224 $^ MissingGeneLocusTag, 156 1225 If one gene in a record has a locus-tag value, all genes in the record 1226 should have a locus-tag value. 1227 1228 $^ MultipleProtRefs, 157 1229 The name and description of the protein is missing from this entry. Every 1230 protein Bioseq must have one full-length Prot-ref feature to provide this 1231 information. 1232 1233 $^ BadInternalCharacter, 158 1234 Biological names or labels should not end with question mark, 1235 exclamation point, or tilde. 1236 1237 $^ BadTrailingCharacter, 159 1238 Biological names or labels should not contain underscore, period, 1239 comma, colon, or semicolon, 1240 1241 $^ BadTrailingHyphen, 160 1242 Biological names or labels should not end with a hyphen. 1243 1244 $^ MultipleGeneOverlap, 161 1245 This genes completely contains two or more other genes. 1246 1247 $^ BadCharInAuthorLastName, 162 1248 An author name has illegal characters. 1249 1250 $^ PseudoCDSmRNArange, 163 1251 A pseudo CDS is overlapped by an mRNA feature, but the mRNA does not cover all 1252 intervals (i.e., exons) on the CDS. This may be an annotation error. 1253 1254 $^ ExtendablePartialProblem, 164 1255 A partial end of this feature does not abut a gap and does not include the first 1256 or last nucleotide in the sequence, but it could be extended by one or two 1257 nucleotides to do so. 1258 1259 $^ GeneXrefNeeded, 165 1260 This feature has multiple overlapping genes of the same length and same name, 1261 but no xref to determine which one should be used for the /gene qualifier. 1262 1263 $^ RubiscoProblem, 166 1264 A protein name contains ribulose and bisphosphate but does not use 1265 the prefered formal naming convention. 1266 1267 $^ UnqualifiedException, 167 1268 The feature is marked with an automatically supplied exception qualifier 1269 that should be replaced or supplemented with more informative information. 1270 1271 $^ ProteinNameHasPMID, 168 1272 The protein name has (PMID #####) embedded in it. 1273 1274 $^ BadGeneOntologyFormat, 169 1275 A feature has incorrectly labeled fields in a Gene Ontology (GO) term specification. 1276 1277 $^ InconsistentGeneOntologyTermAndId, 170 1278 The same GO term should apply to Gene Ontology (GO) term specifications with the same GO ID. 1279 1280 $^ MultiplyAnnotatedGenes, 171 1281 Two gene features with the same name are annotated at the same location. 1282 1283 $^ ReplicatedGeneSequence, 172 1284 Two gene features with the same name are annotated at different locations, but the 1285 underlying sequence is identical. 1286 1287 $^ ShortIntron, 173 1288 Introns should be longer than 10 nt. 1289 1290 $^ GeneXrefStrandProblem, 174 1291 This feature has a gene xref that points to a gene on the wrong strand. 1292 1293 $^ CDSmRNAXrefLocationProblem, 175 1294 The CDS is not contained within the cross-referenced mRNA. 1295 1296 $^ LocusCollidesWithLocusTag, 176 1297 A gene locus is identical with a gene locus_tag. 1298 1299 $$ SEQ_ALIGN, 6 1300 1301 $^ SeqIdProblem, 1 1302 The seqence referenced by an alignment SeqID is not packaged in the record. 1303 1304 $^ StrandRev, 2 1305 Please contact the sequence database for further help with this error. 1306 1307 $^ DensegLenStart, 3 1308 Please contact the sequence database for further help with this error. 1309 1310 $^ StartLessthanZero, 4 1311 Please contact the sequence database for further help with this error. 1312 1313 $^ StartMorethanBiolen, 5 1314 Please contact the sequence database for further help with this error. 1315 1316 $^ EndLessthanZero, 6 1317 Please contact the sequence database for further help with this error. 1318 1319 $^ EndMorethanBiolen, 7 1320 Please contact the sequence database for further help with this error. 1321 1322 $^ LenLessthanZero, 8 1323 Please contact the sequence database for further help with this error. 1324 1325 $^ LenMorethanBiolen, 9 1326 Please contact the sequence database for further help with this error. 1327 1328 $^ SumLenStart, 10 1329 Please contact the sequence database for further help with this error. 1330 1331 $^ AlignDimSeqIdNotMatch, 11 1332 Please contact the sequence database for further help with this error. 1333 1334 $^ SegsDimSeqIdNotMatch, 12 1335 Please contact the sequence database for further help with this error. 1336 1337 $^ FastaLike, 13 1338 Please contact the sequence database for further help with this error. 1339 1340 $^ NullSegs, 14 1341 Please contact the sequence database for further help with this error. 1342 1343 $^ SegmentGap, 15 1344 Please contact the sequence database for further help with this error. 1345 1346 $^ SegsDimOne, 16 1347 Please contact the sequence database for further help with this error. 1348 1349 $^ AlignDimOne, 17 1350 Please contact the sequence database for further help with this error. 1351 1352 $^ Segtype, 18 1353 Please contact the sequence database for further help with this error. 1354 1355 $^ BlastAligns, 19 1356 BLAST alignments are not desired in records submitted to the sequence database. 1357 1358 $^ PercentIdentity, 20 1359 An acceptable percent identity score is 75 percent or higher. 1360 1361 $^ ShortAln, 21 1362 Alignment is shorter than expected. 1363 1364 $^ UnexpectedAlignmentType 22 1365 Only DenseSeg alignments are expected. 1366 1367 $$ SEQ_GRAPH, 7 1368 1369 $^ GraphMin, 1 1370 The graph minimum value is outside of the 0-100 range. 1371 1372 $^ GraphMax, 2 1373 The graph maximum value is outside of the 0-100 range. 1374 1375 $^ GraphBelow, 3 1376 Some quality scores are below the stated graph minimum value. 1377 1378 $^ GraphAbove, 4 1379 Some quality scores are above the stated graph maximum value. 1380 1381 $^ GraphByteLen, 5 1382 The number of bytes in the quality graph does not correspond to the 1383 stated length of the graph. 1384 1385 $^ GraphOutOfOrder, 6 1386 The quality graphs are not packaged in order - may be due to an old fa2htgs bug. 1387 1388 $^ GraphBioseqLen, 7 1389 The length of the quality graph does not correspond to the length of the Bioseq. 1390 1391 $^ GraphSeqLitLen, 8 1392 The length of the quality graph does not correspond to the length of the 1393 delta Bioseq literal component. 1394 1395 $^ GraphSeqLocLen, 9 1396 The length of the quality graph does not correspond to the length of the 1397 delta Bioseq location component. 1398 1399 $^ GraphStartPhase, 10 1400 The quality graph does not start or stop on a sequence segment boundary. 1401 1402 $^ GraphStopPhase, 11 1403 The quality graph does not start or stop on a sequence segment boundary. 1404 1405 $^ GraphDiffNumber, 12 1406 The number quality graph does not equal the number of sequence segments. 1407 1408 $^ GraphACGTScore, 13 1409 Quality score values for known bases should be above 0. 1410 1411 $^ GraphNScore, 14 1412 Quality score values for unknown bases should not be above 0. 1413 1414 $^ GraphGapScore, 15 1415 Gap positions should not have quality scores above 0. 1416 1417 $^ GraphOverlap, 16 1418 Quality graphs overlap - may be due to an old fa2htgs bug. 1419 1420 $^ GraphBioseqId, 17 1421 Quality graph does not map to Bioseq in record. 1422 1423 $^ GraphACGTScoreMany, 18 1424 Quality score values for known bases should be above 0. 1425 1426 $^ GraphNScoreMany, 19 1427 Quality score values for unknown bases should not be above 0. 1428 1429 $^ GraphLocInvalid, 20 1430 Location for quality score values extends beyond end of sequence. 1431 1432 $$ SEQ_ANNOT, 8 1433 1434 $^ AnnotIDs, 1 1435 Seq-annot.data.ids should only be used for communication between programs. 1436 1437 $^ AnnotLOCs, 2 1438 Seq-annot.data.locs should only be used for communication between programs. 1439
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