Summary
[Wormbase] hoe-1 encodes, by alternative splicing, two isoforms of a putative metal-dependent hydrolase orthologous to human ELAC2 (OMIM:605367, associated with susceptibility to prostate cancer); HOE-1 is required for fertility, normal growth progression during the late larval stages and germ line proliferation; since hoe-1(RNAi) animals do not produce gametes, hoe-1 may be required for mitosis and meiosis; hoe-1 deficiency suppresses the multivulva (Muv) phenotype of activated LET-60/RAS, while having no effect on other RAS pathway members.
Wormbase predicts 2 models, but Caenorhabditis elegans cDNA sequences in GenBank, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 3 spliced variants.

AceView summary
Expression: According to AceView, this gene is well expressed, 1.1 times the average gene in this release, mostly from L1 larvae to adult [Kohara cDNAs]. The sequence of this gene is defined by 14 cDNA clones. We annotate structural defects or features in one cDNA clone.
Alternative mRNA variants and regulation: The gene contains 7 distinct gt-ag introns. Transcription produces 3 alternatively spliced mRNAs. There are 2 probable alternative promotors and 3 validated alternative polyadenylation sites (see the diagram).
Protein coding potential: The 3 spliced mRNAs putatively encode good proteins, altogether 2 different isoforms (2 complete, 2 COOH complete, 1 partial), some containing beta-lactamase-like domain [Pfam], a coiled coil stretch [Psort2].
Function: There is one article specifically referring to this gene in PubMed. This gene is associated to a phenotype (LONg body length, SLow growth). Proteins are expected to have molecular function (hydrolase activity) and to localize in cytoplasm.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Map: This gene hoe-1 maps on chomosome IV at position +1.17 (interpolated). In AceView, it covers 4.79 kb, from 4744258 to 4739474 (WS190), on the reverse strand.
Links to: WormBase, NextDB, RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 4F504, in Wormbase by its cosmid.number name E04A4.4, in NextDB, the Nematode expression pattern database, as CEYK89.

The closest human gene, according to BlastP, is the AceView gene ELAC2 (e=9 10^-69).
The closest mouse gene, according to BlastP, is the AceView gene Elac2 (e=4 10^-71).
The closest A.thaliana genes, according to BlastP, are the AceView genes AT3G16260 (e=2 10^-57), AT1G52160 (e=3 10^-56)

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNA clones supporting each intron. Superimposed introns of the same color are identical, of different colors are different.
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Details on tissue of origin for each intron and exon is available from the intron and exons table. Good predicted proteins are in pink, yellow proteins may be partial or unconvincing, green are uORFs. Proteins supported by a single continuous GenBank accession lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
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