Summary
[Wormbase] gst-1 encodes a putative glutathione S-transferase of the pi class; GST-1 is required for sperm to normally migrate towards a PUFA-based signal exuded by oocytes; gst-1(RNAi) hermaphrodites are infertile, both through aberrant loss of their own sperm and through failure of males to effectively inseminate them
.
Wormbase predicts one model.
AceView summary
According to AceView, this gene is
well expressed, 1.2 times the average gene in this release, mostly in embryos, and some at all stages of development [Kohara cDNAs]. The
sequence of this gene is defined by
16 cDNA clones, some from whole worm (seen once).
The gene contains
2 distinct gt-ag introns. Transcription produces one mRNA. There are 2 validated
alternative polyadenylation sites (see the
diagram).
The spliced mRNA putatively encodes
a good protein, containing
domains glutathione S-transferase, C-terminal, Glutathione S-transferase, N-terminal [Pfam].
Function: There are
2 articles specifically referring to this gene in PubMed. In addition we point
below to 3 abstracts. Proteins are expected to
localize in cytoplasm. No phenotype has yet been reported to our knowledge: this gene's in vivo function is yet unknown.
Please quote:
AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map: This gene gst-1 maps on chomosome III at position +0.08 (interpolated). In AceView, it covers
0.86 kb, from 9059242 to 9060098 (WS190), on the direct strand.
Links to: WormBase,
NextDB,
RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 3K149, in Wormbase by its cosmid.number name R107.7, in NextDB, the Nematode expression pattern database, as CEYK3081.
The closest human gene, according to BlastP, is the AceView gene
GSTP1 (e=2 10^-37).
The closest mouse gene, according to BlastP, is the AceView gene
Gstp2andGstp1 (e=5 10^-44).
The closest A.thaliana genes, according to BlastP, are the AceView genes
AT5G52940 (e=0.20),
ATGSTF12 (e=0.28)
Legend
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron.
]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron).
] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries.
]-[ Pink and
] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal,
blue flags to any single letter variant of the main . More explanations are given in the
gene help file
