Summary
[Wormbase] gpd-4 encodes one of four C. elegans glyceraldehyde-3-phosphate dehydrogenases (GAPDHs); by homology, GPD-4 is predicted to reversibly catalyze the oxidation and phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate during glycolysis; GPD-4 is required for embryonic and larval development; in C. elegans, GPD-4 and GPD-1 constitute the minor GAPDH isoenzymes and are expressed primarily in embryos, in all cell types
.
Wormbase predicts one model.
AceView summary
According to AceView, this gene is
expressed at high level, 1.7 times the average gene in this release, in L3, L4, adult and culminating in embryos [Kohara cDNAs]. The
sequence of this gene is defined by
22 cDNA clones. We annotate
structural defects or features in one cDNA clone.
The gene contains
2 distinct gt-ag introns. Transcription produces one mRNA.
The spliced mRNA putatively encodes
a good protein, containing
domains Glyceraldehyde 3-phosphate dehydrogenase, Glyceraldehyde 3-phosphate dehydrogenase [Pfam].
Function: There are
5 articles specifically referring to this gene in PubMed. In addition we point
below to 2 abstracts. This essential gene is associated to a
phenotype (Embryonic Lethal, Larval arrest, oogenic protein copurified with chromatin). Functionally, the gene has been proposed to participate in a
process (glucose metabolism). Proteins are expected to have molecular
function (glyceraldehyde 3-phosphate dehydrogenase (phosphorylating) activity) and to
localize in cytoplasm.
Please quote:
AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map: This essential gene gpd-4 maps on chomosome II at position +2.07 (interpolated). In AceView, it covers
1.23 kb, from 10171192 to 10172422 (WS190), on the direct strand.
Links to: WormBase,
NextDB,
RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 2K461, in Wormbase by its cosmid.number name F33H1.2, in NextDB, the Nematode expression pattern database, as CEYK2502.
The closest human genes, according to BlastP, are the AceView genes
GAPDH (e= 10^-144),
GAPDHS (e= 10^-131),
GAPDHP1 (e= 10^-131).
The closest mouse genes, according to BlastP, are the AceView genes
LOC626807 (e= 10^-146),
LOC380687 (e= 10^-146),
Gapdh.1 (e= 10^-146),
Gapdh (e= 10^-146),
LOC654475 (e= 10^-146),
LOC654473 (e= 10^-146),
LOC654474 (e= 10^-146),
LOC433921 (e= 10^-145),
OTTMUSG00000005300 (e= 10^-145),
EG277333 (e= 10^-137),
EG629557 (e= 10^-136),
Gapdhs (e= 10^-129).
The closest A.thaliana genes, according to BlastP, are the AceView genes
GAPCP-2 (e= 10^-130),
GAPCP-1 (e= 10^-130),
GAPC-2 (e= 10^-127),
GAPC (e= 10^-130)
Legend
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron.
]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron).
] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries.
]-[ Pink and
] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal,
blue flags to any single letter variant of the main . More explanations are given in the
gene help file
