Summary
[Wormbase] fos-1 encodes two basic region-leucine zipper (bZip) transcription factors, FOS-1A and FOS-1B, that are the sole C. elegans orthologs of the fos bZip transcription factor family; during hermaphrodite development, FOS-1A activity is required cell autonomously in the gonadal anchor cell for basement-membrane removal and subsequent anchor cell invasion of the vulval epithelium; in addition, fos-1 activity is also required for proper vulval and uterine development, fertility, and oogenesis; in late-L3 larvae, a FOS-1A translational fusion protein is expressed at high levels in the anchor cell nucleus and at lower levels in uterine cells, while a FOS-1B reporter is expressed at low levels in the anchor cell, uterine, and vulval cells; transcriptional reporters additionally reveal that fos-1b is expressed in most cells of late L3 larvae; in affecting anchor cell invasion, FOS-1A appears to act by regulating the expression of three AC-expressed genes: cdh-3/cadherin, him-4/hemicentin, and zmp-1/matrix metalloproteinase, which likely function together to promote anchor cell invasion
.
Wormbase predicts 2 models from 2 genes.
AceView summary
Expression: According to AceView, this gene is
well expressed, 0.6 times the average gene in this release, In the L3 larva, fos-1a is expressed transcriptionally and translationally mainly in the anchor cell and less strongly in the neighboring uterine cells. It is absent from the vulval precursor cells. This is in contrast to fos-1b which at that stage is expressed in most cells (peak in phasmids and some central nerve cells). FOS-1b protein would be made in many cells including the vulval precursor cells (caveat: the CFP was inserted in first exon, way ahead of the predicted ATG) [from Sherwood et al, 2005]. The
sequence of this gene is defined by
8 cDNA clones.
Alternative mRNA variants and regulation: The gene contains
7 distinct gt-ag introns. Transcription produces
2 alternatively spliced mRNAs. There are 2 validated
alternative polyadenylation sites (see the
diagram). 352 bp of this gene
are antisense to spliced gene rpt-2, raising the possibility of regulated alternate expression.
Protein coding potential: The 2 spliced mRNAs putatively encode
good proteins, altogether
3 different isoforms (1 complete, 3 COOH complete), some containing
domains bZIP transcription factor, bZIP-1, basic leucine zipper [Pfam], a coiled coil stretch
[Psort2].
Function: There are
4 articles specifically referring to this gene in PubMed. In addition we point
below to 2 abstracts. This essential gene is associated to a
phenotype (abnormal Eversion of VuLva, fragile animal, may explode at vulva, Protruding VuLva, Sterile adult, defect in invasion of vulval cells by anchor cell). Functionally, the gene has been proposed to participate in a
process (regulation of transcription, DNA-dependent). Proteins are expected to have molecular
functions (protein dimerization activity, transcription factor activity) and to
localize in nucleus.
Please quote:
AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map: This essential gene fos-1 maps on chomosome V at position -0.80 (interpolated). In AceView, it covers
12.81 kb, from 6003584 to 6016395 (WS190), on the direct strand.
Links to: WormBase,
NextDB,
RNAiDB.
as
Other names: The gene is also known evl-5, in Wormgenes/AceView by its positional name 5G495, in Wormbase by its cosmid.number name F29G9.4, in NextDB, the Nematode expression pattern database, as CEYK7178.
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where
introns have been shrunk to a minimal length. Exon size is proportional to length,
intron height reflects the number of cDNA clones supporting each intron. Superimposed introns of the same color are identical, of different colors are different.
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Details on tissue of origin for each intron and exon is available from the
intron and exons table. Good predicted proteins are in pink, yellow proteins may be partial or unconvincing, green are uORFs. Proteins supported by a single continuous GenBank accession lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
More legend
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron.
]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron).
] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries.
]-[ Pink and
] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal,
blue flags to any single letter variant of the main . More explanations are given in the
gene help file
