Summary
[Wormbase] emb-30 encodes an anaphase-promoting complex/cyclosome (APC/C) component orthologous to mammalian APC-4 and Schizosaccharomyces pombe Lid1; EMB-30 is required for the metaphase-to-anaphase transition during meiosis and mitosis, for establishing anterior-posterior polarity in the early embryo, and for proper localization of germline granules and the maternally provided PAR-2 and PAR-3 proteins.
Wormbase predicts one model, but Caenorhabditis elegans cDNA sequences in GenBank, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 2 spliced variants.

AceView summary
Expression: According to AceView, this gene is expressed at high level, 1.7 times the average gene in this release, at all stages of development [Kohara cDNAs]. The sequence of this gene is defined by 22 cDNA clones.
Alternative mRNA variants and regulation: The gene contains 14 distinct gt-ag introns. Transcription produces 2 alternatively spliced mRNAs. There are 8 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by by overlapping exons with different boundaries, splicing versus retention of one intron.
Protein coding potential: The 2 spliced mRNAs putatively encode good proteins, altogether 2 different isoforms (1 complete, 2 COOH complete).
Function: There are 7 articles specifically referring to this gene in PubMed. In addition we point below to 23 abstracts. This essential gene is associated to a phenotype (abnormal EMBryogenesis, catastrophic one cell arrest, Embryonic Lethal, Metaphase-to-Anaphase Transition defect, NO Pseudocleavage, osmotic or pressure sensitive, Polarity and Osmotic sensitivity Defect, Progress through meiotic divisions, Required for metaphase to anaphase transitions during mitosis and meiosis, polar bodies (female meiosis products) abnormal). Functionally, the gene has been proposed to participate in a process (pseudocleavage (sensu Nematoda)).

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Map: This essential gene emb-30 maps on chomosome III at position +0.37 (interpolated). In AceView, it covers 6.39 kb, from 9441364 to 9434978 (WS190), on the reverse strand.
Links to: WormBase, NextDB, RNAiDB.
as Other names: The gene is also known apc-4, in Wormgenes/AceView by its positional name 3K526, in Wormbase by its cosmid.number name F54C8.3, in NextDB, the Nematode expression pattern database, as CEYK3482.

The closest human genes, according to BlastP, are the AceView genes WDR61 (e=0.003), EML5 (e=0.012), ANAPC4 (e=0.019).
The closest mouse genes, according to BlastP, are the AceView genes Anapc4 (e=8 10^-04), Wdr61 (e=0.002), Eml5 (e=0.008).
The closest A.thaliana gene, according to BlastP, is the AceView gene AT4G21530andAT4G21540 (e=3 10^-06)

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNA clones supporting each intron. Superimposed introns of the same color are identical, of different colors are different.
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Details on tissue of origin for each intron and exon is available from the intron and exons table. Good predicted proteins are in pink, yellow proteins may be partial or unconvincing, green are uORFs. Proteins supported by a single continuous GenBank accession lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
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