Summary
[Wormbase] dpy-27 encodes an ATP-binding protein that is a homolog of the SMC4 subunit of mitotic condensin; DPY-27, in combination with other proteins including MIX-1, act as a unit to repress X-linked gene expression during hermaphrodite dosage compensation; in XX oocytes and early embryos, DPY-27 exhibits diffuse nuclear localization, but by the 30-cell stage of embryogenesis, DPY-27 specifically localizes to X chromosomes; in XO animals at all stages, DPY-27 remains diffusely nuclear; the sex-specific localization of DPY-27 to X chromosomes is dependent upon wild-type activity of xol-1, as DPY-27 mislocalizes to the X chromosome of XO embryos in a xol-1 mutant background
.
Wormbase predicts one model.
AceView summary
According to AceView, this gene is
expressed at high level, 2.0 times the average gene in this release, at all stages of development [Kohara cDNAs]. The
sequence of this gene is defined by
26 cDNA clones, some from whole animal (seen once).
The gene contains
11 distinct gt-ag introns. Transcription produces one mRNA. There are 4 validated
alternative polyadenylation sites (see the
diagram).
The spliced mRNA putatively encodes
a good protein, containing
domains SMCs flexible hinge, RecF/RecN/SMC protein, N-terminal [Pfam], a second peroximal domain, a coiled coil stretch
[Psort2].
Function: There are
12 articles specifically referring to this gene in PubMed. In addition we point
below to 49 abstracts. This essential gene is associated to a
phenotype (DumPY : shorter than wild-type, EGg Laying defective, embryonic lethal, partial, Larval arrest, Sterile adult, UNCoordinated locomotion, X chromosome dosage compensation defect, affects axonal growth or guidance). Proteins are expected to have molecular
functions (ATP binding activity, protein binding activity) and to
localize in chromosome.
Please quote:
AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map: This essential gene dpy-27 maps on chomosome III at position -5.12 (interpolated). In AceView, it covers
6.14 kb, from 3819658 to 3813515 (WS190), on the reverse strand.
Links to: WormBase,
NextDB,
RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 3E904, in Wormbase by its cosmid.number name R13G10.1, in NextDB, the Nematode expression pattern database, as CEYK342.
The closest human gene, according to BlastP, is the AceView gene
SMC4 (e= 10^-132).
The closest mouse gene, according to BlastP, is the AceView gene
Smc4 (e=3 10^-82).
The closest A.thaliana gene, according to BlastP, is the AceView gene
ATSMC3 (e=9 10^-52)
Legend
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron.
]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron).
] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries.
]-[ Pink and
] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal,
blue flags to any single letter variant of the main . More explanations are given in the
gene help file
