Summary
[Wormbase] dbr-1 encodes a homolog of the RNA lariat-debranching enzyme; DBR-1 is predicted to function in RNA processing by hydrolyzing the 2'-5' phosphodiester bond at the branchpoint of excised lariat intron RNA, thus converting the introns into linear molecules that can subsequently be degraded by exonucleases; in C. elegans, DBR-1 activity is required for embryonic and germline development, as well as for normal rates of postembryonic growth; overexpression of C. elegans DBR-1 in Saccharomyces cerevisiae can complement the RNA processing defects of a dbr1 null mutant strain, restoring debranching activity and intron degradation to near wild-type levels.
Wormbase predicts one model, but Caenorhabditis elegans cDNA sequences in GenBank, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 2 spliced variants.

AceView summary
Expression: According to AceView, this gene is well expressed, 1.3 times the average gene in this release, mostly from L1 larvae to adult [Kohara cDNAs]. The sequence of this gene is defined by 17 cDNA clones, some from whole animal (seen 2 times).
Alternative mRNA variants and regulation: The gene contains 5 distinct introns (4 gt-ag, 1 gc-ag). Transcription produces 2 alternatively spliced mRNAs. There are 3 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by by overlapping exons with different boundaries, splicing versus retention of one intron.
Protein coding potential: The 2 spliced mRNAs putatively encode good proteins, altogether 2 different isoforms (2 complete, 1 COOH complete), some containing domains Lariat debranching enzyme, C-terminal, Metallophosphoesterase [Pfam].
Function:. This essential gene is associated to a phenotype (embryonic lethal, partial, SLow growth). Functionally, the gene has been proposed to participate in a process (mRNA processing). Proteins are expected to have molecular functions (hydrolase activity, hydrolase activity, acting on ester bonds) and to localize in nucleus.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Map: This essential gene dbr-1 maps on chomosome I at position +1.14 (interpolated). In AceView, it covers 2.01 kb, from 6492729 to 6490718 (WS190), on the reverse strand.
Links to: WormBase, NextDB, RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 1H68, in Wormbase by its cosmid.number name C55B7.8, in NextDB, the Nematode expression pattern database, as CEYK9467.

The closest human gene, according to BlastP, is the AceView gene DBR1 (e=4 10^-23).
The closest mouse gene, according to BlastP, is the AceView gene Dbr1 (e=8 10^-22).
The closest A.thaliana gene, according to BlastP, is the AceView gene AT4G31770 (e=3 10^-16)

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNA clones supporting each intron. Superimposed introns of the same color are identical, of different colors are different.
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Details on tissue of origin for each intron and exon is available from the intron and exons table. Good predicted proteins are in pink, yellow proteins may be partial or unconvincing, green are uORFs. Proteins supported by a single continuous GenBank accession lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
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