Summary
[Wormbase] cpl-1 encodes a member of the cathepsin L-like cysteine protease family required for embryonic viability and normal growth; expressed in eggshells and throughout early embryos, accumulates in intestinal cells during late embryogenesis, and expressed in the cuticle, gonad, and pharynx later in development
.
Wormbase predicts one model.
AceView summary
According to AceView, this gene is
expressed at very high level, 4.6 times the average gene in this release, at all stages of development [Kohara cDNAs]. The
sequence of this gene is defined by
60 cDNA clones, some from whole animal (seen 2 times). We annotate
structural defects or features in 4 cDNA clones.
The gene contains
3 distinct gt-ag introns. Transcription produces one mRNA. There are 2 validated
alternative polyadenylation sites (see the
diagram).
The spliced mRNA putatively encodes
a good protein, containing
domains Proteinase inhibitor I29, cathepsin propeptide, peptidase C1A, papain C-terminal [Pfam], a coiled coil stretch
[Psort2]; the complete protein appears to be
secreted.
Function: There are
3 articles specifically referring to this gene in PubMed. In addition we point
below to 2 abstracts. This essential gene is associated to a
phenotype (embryonic lethal, partial, SLow growth, shared oogenic and spermatogenic protein copurified with chromatin). Functionally, the gene has been proposed to participate in a
process (proteolysis and peptidolysis). Proteins are expected to have molecular
function (cysteine-type peptidase activity) and to
localize in extracellular space.
Please quote:
AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map: This essential gene cpl-1 maps on chomosome V at position +9.40 (interpolated). In AceView, it covers
2.78 kb, from 16595318 to 16592537 (WS190), on the reverse strand.
Links to: WormBase,
NextDB,
RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 5R74, in Wormbase by its cosmid.number name T03E6.7, in NextDB, the Nematode expression pattern database, as CEYK2090.
The closest human genes, according to BlastP, are the AceView genes
CTSL1 (e=2 10^-89),
CTSL2 (e=5 10^-86),
CTSS (e=6 10^-79).
The closest mouse genes, according to BlastP, are the AceView genes
Ctsl (e=6 10^-87),
Ctss (e=7 10^-79),
2310051M13Rik (e=9 10^-79),
BC051665 (e=3 10^-77),
Ctsk (e=2 10^-76),
4930486L24Rik (e=4 10^-76).
The closest A.thaliana genes, according to BlastP, are the AceView genes
AT5G43060 (e=4 10^-67),
AT3G19390 (e=10^-64),
RD21 (e=2 10^-64),
AT3G48340 (e=4 10^-63),
AALP (e=9 10^-63),
CP1 (e=2 10^-62),
XBCP3 (e=10^-61),
AT3G48350 (e=6 10^-61),
SAG12 (e=10^-60),
AT5G50260 (e=10^-60),
AT3G19400 (e=2 10^-60),
XCP2 (e=3 10^-60),
AT3G45310 (e=5 10^-60)
Legend
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron.
]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron).
] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries.
]-[ Pink and
] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal,
blue flags to any single letter variant of the main . More explanations are given in the
gene help file
