Summary
[Wormbase] ced-4 encodes a novel protein; along with CED-3, CED-4 is required for the initiation of programmed cell death; accordingly, genetic analyses indicate that ced-3 and ced-4 function upstream of ced-1, ced-2, and nuc-1 in the programmed cell death pathway; in yeast two-hybrid experiments, and upon coexpression in mammalian cells, CED-4 interacts with CED-9, an anti-apoptotic BCL-2 homolog; coexpression of CED-4 and CED-9 results in redistribution of CED-4 from the cytosol to organellar membranes, suggesting that CED-9 may negatively regulate CED-4 activity by sequestering CED-4 to intracellular membranes
.
Wormbase predicts 2 models.
AceView summary
According to AceView, this gene is
well expressed, 0.9 times the average gene in this release, at all stages of development [Kohara cDNAs]. The
sequence of this gene is defined by
12 cDNA clones.
The gene contains
7 distinct gt-ag introns. Transcription produces one mRNA.
The spliced mRNA putatively encodes
a good protein, altogether
2 different isoforms (1 complete, 2 COOH complete), some containing
domains Caspase Recruitment, NB-ARC [Pfam].
Function: There are
95 articles specifically referring to this gene in PubMed. In addition we point
below to 109 abstracts. This gene is associated to a
phenotype (CEll Death abnormality). Functionally, the gene has been proposed to participate in
processes (apoptosis, regulation of apoptosis). Proteins are expected to have molecular
functions (ATP binding activity, protein binding activity) and to
localize in various compartments (cytoplasm, intracellular).
Please quote:
AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map: This gene ced-4 maps on chomosome III at position -2.32 (interpolated). In AceView, it covers
2.89 kb, from 4852288 to 4855172 (WS190), on the direct strand.
Links to: WormBase,
RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 3F943, in Wormbase by its cosmid.number name C35D10.9.
Legend
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron.
]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron).
] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries.
]-[ Pink and
] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal,
blue flags to any single letter variant of the main . More explanations are given in the
gene help file
