Summary
The gene ace-2 encodes acetylcholinesterase class B, and produces about 45% of the nematode acetylcholinesterase activity [Johnson and Russell, 1983]. The C terminus of ACE-2 is hydrophobic, of H type; it associates into glycolipid-anchored dimers [Combes et al, 2000]. ace-2 is partly redundant functionnally with ace-1: the double mutant ace-1 ace-2 has uncoordinated locomotion, moving slowly forward and hypercontracted backward [Kolson and Russell, 1985]. Absence of the three classes A B C of acetylcholinesterases together, in the ace-1 ace-2 ace-3 triple mutant, leads to paralysis and late embryonic/early larval lethality [Johnson et al, 1988]. ace-2 is expressed at low level in larvae, in overlap with ace-1, but also specifically in the pharyngeo-intestinal valve where ace-1 is not expressed [Combes et al, 2000][Wormbase] An acetylcholineesterase involved in the termination of cholinergic nerve transmission; it is predominantly expressed in motoneurons
.
Wormbase predicts one model.
AceView summary
According to AceView, this gene is
moderately expressed, only 38.0% of the average gene in this release, in L1, L2 and L3 larvae [Kohara cDNAs]. The
sequence of this gene is defined by
5 cDNA clones, some from whole animal (seen once).
The gene contains
8 distinct gt-ag introns. Transcription produces one mRNA. There are 2 validated
alternative polyadenylation sites (see the
diagram).
The spliced mRNA putatively encodes
a good protein, containing Carboxylesterase, type B
domain [Pfam], some transmembrane domains
[Psort2].
Function: There are
20 articles specifically referring to this gene in PubMed. In addition we point
below to 21 abstracts. This gene is associated to a
phenotype (abnormal ACEtylcholinesterase). Proteins are expected to
localize in membrane.
Please quote:
AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map: This gene ace-2 maps on chomosome I at position -4.04 (interpolated). In AceView, it covers
5.98 kb, from 3307466 to 3301489 (WS190), on the reverse strand.
Links to: WormBase,
RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 1D872, in Wormbase by its cosmid.number name Y44E3A.2.
The closest human gene, according to BlastP, is the AceView gene
BCHE (e=6 10^-99).
The closest mouse genes, according to BlastP, are the AceView genes
Bche (e=9 10^-97),
Acheand2700038N03Rik (e=2 10^-85).
The closest A.thaliana genes, according to BlastP, are the AceView genes
AT3G02410 (e=2 10^-04),
ATPCME (e=3 10^-04)
Legend
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron.
]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron).
] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries.
]-[ Pink and
] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal,
blue flags to any single letter variant of the main . More explanations are given in the
gene help file
