Homo sapiens complex locus DUSP1, encoding dual specificity phosphatase 1.
TABLE OF CONTENTS / OPEN CLOSE ALL PARAGRAPHS
SUMMARY back to top
RefSeq summary
[DUSP1] The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq].

RefSeq annotates one representative transcript (NM included in AceView variant.a), but Homo sapiens cDNA sequences in GenBank, dbEST, Trace and SRA, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 6 spliced variants.

AceView synopsis, each blue text links to tables and details
Note that this locus is complex: it appears to produce several proteins with no sequence overlap.
Expression: According to AceView, this gene is expressed at high level, 2.7 times the average gene in this release. The sequence of this gene is defined by 695 GenBank accessions, some from lung (seen 34 times), eye (27), brain (20), prostate (12), alveolar macrophage (10), anaplastic oligodendroglioma (9), skin (9) and 104 other tissues. We annotate structural defects or features in 2 cDNA clones.
Alternative mRNA variants and regulation: The gene contains 5 distinct introns (4 gt-ag, 1 gc-ag). Transcription produces 7 different mRNAs, 6 alternatively spliced variants and 1 unspliced form. There are 2 probable alternative promotors, 2 non overlapping alternative last exons and 6 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of a cassette exon, splicing versus retention of 3 introns.
Function: There are 84 articles specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to diseases (Breast Neoplasms; Carcinoma, Hepatocellular; Ovarian Neoplasms), proposed to participate in pathways (CD40L Signaling Pathway, MAPK signaling pathway, Mechanism of Gene Regulation by Peroxisome Proliferators via PPARa(alpha), NFkB activation by Nontypeable Hemophilus influenzae, TNFR2 Signaling Pathway) and processes (cell cycle, cellular response to hormone stimulus, positive regulation of anti-apoptosis, positive regulation of apoptosis, protein amino acid dephosphorylation, response to calcium ion, response to cAMP, response to estradiol stimulus, response to glucocorticoid stimulus, response to hydrogen peroxide, response to light stimulus, response to retinoic acid, response to testosterone stimulus). Proteins are expected to have molecular functions (hydrolase activity, MAP kinase tyrosine/serine/threonine phosphatase activity, protein tyrosine/threonine phosphatase activity) and to localize in various compartments (cytoplasm, nucleus, soluble fraction). Putative protein interactors have been described (CKS1B, HSPA4, MAPK1, MAPK3, MAPK8, MAPK9, MAPK10, MAPK12ANDHDAC10, MAPK14, SKP2, UBB, UBCANDSCARB1).
Protein coding potential: 5 spliced and the unspliced mRNAs putatively encode good proteins, altogether 6 different isoforms (3 complete, 2 COOH complete, 1 partial), some containing domains Dual specificity phosphatase, catalytic domain, Rhodanese-like domain, Protein-tyrosine phosphatase [Pfam]. The remaining mRNA variant (spliced; partial) appears not to encode a good protein. Finally proteins from this gene may be modulated by acetylation; phosphorylation; ubiquitination, as detailed at PhosphoSite.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12.
Map on chromosome 5, links to other databases and other names
Map: This gene DUSP1 maps on chromosome 5, at 5q34 according to Entrez Gene. In AceView, it covers 4.24 kb, from 172198230 to 172193996 (NCBI 37, August 2010), on the reverse strand.
Links to: manual annotations from KEGG_04010, PhosphoSite, the SNP view, gene overviews from Entrez Gene 1843, GeneCards, expression data from ECgene, UniGene, molecular and other annotations from UCSC, or our GOLD analysis.
The previous AceView annotation is here.
Other names: The gene is also known as DUSP1, HVH1, MKP1, CL100, MKP-1, PTPN10 or LOC1843, myskerby. It has been described as dual specificity protein phosphatase 1, MAP kinase phosphatase 1, protein-tyrosine phosphatase CL100, dual specificity protein phosphatase hVH1, serine/threonine specific protein phosphatase, mitogen-activated protein kinase phosphatase 1.
EC number: This gene encodes proteins number: 3.1.3.48, 3.1.3.16.
Closest AceView homologs in other species ?
The closest mouse gene, according to BlastP, is the AceView gene Dusp1 (e=10-78).
The closest C.elegans gene, according to BlastP, is the AceView/WormGene nrf-2 (e=2 10-05), which may contain interesting functional annotation.
The closest A.thaliana genes, according to BlastP, are the AceView genes AT3G23610 (e=10-15), IBR5 (e=6 10-15), MKP2 (e=8 10-15), PHS1 (e=8 10-13), which may contain interesting functional annotation
RNA_seq discoveries back to top
Expression/conservation in primates tissues evaluated by cross-mapping to human. back to top
RNA-seq gene expression profile across 16 selected tissues from the Non-Human Primates Reference Transcriptome Resource (link to NHPRTR project).
- Primates: Apes (HUM: Human (Illumina BodyMap 2), CHP: Chimpanzee), Old World monkeys (PTM: Pig-Tailed Macaque, JMI Japanese Macaque, RMI Rhesus Macaque Indian, RMC Rhesus Macaque Chinese, CMM Cynomolgus Macaque Mauritian, CMC Cynomolgus Macaque Chinese, BAB Olive Baboon, SMY Sooty Mangabey); New World monkeys (MST common Marmoset, SQM Squirrel Monkey, OWL Owl Monkey); and Lemurs (MLM Mouse Lemur, RTL Ring-Tailed Lemur).
- The level for significantly expressed genes is color coded in 8 equal sized bins (light to dark green). Light gray is for weak not-accurately measured expression (2 to 8 reads above intergenic background); dark gray for no expression or no sequence conservation (0 read in gene). The plot to the right shows the distribution of measured expression values in all tissues for all genes (blue) and for this gene (green), in Magic index = log2(1000 sFPKM).
You may also examine the strand-specific genome coverage plots on the experimental AceView/Magic hub at UCSC, by tissue or by species. Tracks may be slow to load; please reload if some tracks come up yellow-greenish, and thanks to UCSC for the great work!.
Read more...
          Complete gene on genome diagram: back to top
Please choose between the zoomable GIF version., and the Flash version.
This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones.
Compact gene diagram back to top
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Read more...
Sequences: click on the numbers to get the DNA back to top
mRNA variant mRNA matching the genome Best predicted protein 5' UTR 3' UTR Upstream sequence Transcription
unit
pre-mRNA
Downstream sequence
aAug10 2049 bp 447 aa 275 bp 670 bp 2kb including Promoter 3136 bp 1kb
bAug10 1773 bp 270 aa 958 bp 2kb probably including promoter 2052 bp 1kb
cAug10 2831 bp 255 aa 294 bp 1769 bp 2kb possibly including promoter 3639 bp 1kb
dAug10-unspliced 3096 bp 276 aa 242 bp 2263 bp 2kb including Promoter 3096 bp 1kb
eAug10 560 bp 119 aa 201 bp 2kb possibly including promoter 1002 bp 1kb
fAug10 645 bp 75 aa 417 bp 2kb 1673 bp 1kb
gAug10 665 bp 37 aa 551 bp 2kb 793 bp 1kb

Gene neighbors and Navigator on chromosome 5q34 back to top
ZOOM IN                D:disease,C:conserved,I:interactions,R:regulation,P:publications         Read more...
Annotated mRNA diagrams back to top
Bibliography:               84 articles in PubMed back to top
? Gene Summary Gene on genome mRNA:.a, .b, .c, .d-u, .e, .f, .g Alternative mRNAs features, proteins, introns, exons, sequences Expression Tissue Function, regulation, related genes DCI

To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To compare alternative variants, their summarized annotations, predicted proteins, introns and exons, or to access any sequence, click the 'Alternative mRNAs features' tab. To see a specific mRNA variant diagram, sequence and annotation, click the variant name in the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.

If you know more about this gene, or found errors, please share your knowledge. Thank you !