[DUSP1] The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq].
RefSeq annotates one representative transcript (NM included in AceView variant.a), but Homo sapiens cDNA sequences in GenBank, dbEST, Trace and SRA, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 6 spliced variants
AceView synopsis, each blue text links to tables and details
Note that this locus is complex: it appears to produce several proteins with no sequence overlap.
According to AceView, this gene is expressed at high level
, 2.7 times the average gene in this release. The sequence
of this gene is defined by 695 GenBank accessions
, some from lung (seen 34 times), eye (27), brain (20), prostate (12), alveolar macrophage (10), anaplastic oligodendroglioma (9), skin (9) and 104 other tissues
. We annotate structural defects or features
in 2 cDNA clones.
Alternative mRNA variants and regulation:
The gene contains 5 distinct introns
(4 gt-ag, 1 gc-ag). Transcription produces 7 different mRNAs
, 6 alternatively spliced variants and 1 unspliced form. There are 2 probable alternative promotors
, 2 non overlapping alternative last exons and 6 validated alternative polyadenylation sites
(see the diagram
). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of a cassette exon
, splicing versus retention of 3 introns.
There are 84 articles
specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to diseases
(Breast Neoplasms; Carcinoma, Hepatocellular; Ovarian Neoplasms), proposed to participate in pathways
(CD40L Signaling Pathway, MAPK signaling pathway, Mechanism of Gene Regulation by Peroxisome Proliferators via PPARa(alpha), NFkB activation by Nontypeable Hemophilus influenzae, TNFR2 Signaling Pathway) and processes
(cell cycle, cellular response to hormone stimulus, positive regulation of anti-apoptosis, positive regulation of apoptosis, protein amino acid dephosphorylation, response to calcium ion, response to cAMP, response to estradiol stimulus, response to glucocorticoid stimulus, response to hydrogen peroxide, response to light stimulus, response to retinoic acid, response to testosterone stimulus). Proteins are expected to have molecular functions
(hydrolase activity, MAP kinase tyrosine/serine/threonine phosphatase activity, protein tyrosine/threonine phosphatase activity) and to localize
in various compartments (cytoplasm, nucleus, soluble fraction). Putative protein interactors
have been described (CKS1B, HSPA4, MAPK1, MAPK3, MAPK8, MAPK9, MAPK10, MAPK12ANDHDAC10, MAPK14, SKP2, UBB, UBCANDSCARB1).
Protein coding potential:
5 spliced and the unspliced mRNAs putatively encode good proteins
, altogether 6 different isoforms (3 complete, 2 COOH complete, 1 partial
), some containing domains
Dual specificity phosphatase, catalytic domain, Rhodanese-like domain, Protein-tyrosine phosphatase [Pfam]. The remaining mRNA variant (spliced; partial) appears not to encode a good protein. Finally proteins from this gene may be modulated by acetylation; phosphorylation; ubiquitination
, as detailed at PhosphoSite.
Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map on chromosome 5, links to other databases and other names
This gene DUSP1 maps on chromosome 5, at 5q34 according to Entrez Gene. In AceView, it covers 4.24 kb
, from 172198230 to 172193996 (NCBI 37, August 2010), on the reverse strand.
manual annotations from KEGG_04010
, the SNP
view, gene overviews from Entrez Gene 1843
, expression data from ECgene
, molecular and other annotations from UCSC
, or our GOLD
The previous AceView annotation is here
The gene is also known as DUSP1, HVH1, MKP1, CL100, MKP-1, PTPN10 or LOC1843, myskerby. It has been described as dual specificity protein phosphatase 1, MAP kinase phosphatase 1, protein-tyrosine phosphatase CL100, dual specificity protein phosphatase hVH1, serine/threonine specific protein phosphatase, mitogen-activated protein kinase phosphatase 1.
This gene encodes proteins number: 126.96.36.199, 188.8.131.52.
Closest AceView homologs in other species
The closest mouse gene
, according to BlastP, is the AceView gene Dusp1
The closest C.elegans gene
, according to BlastP, is the AceView/WormGene nrf-2
), which may contain interesting functional annotation.
The closest A.thaliana genes
, according to BlastP, are the AceView genes AT3G23610
), which may contain interesting functional annotation
Complete gene on genome diagram:
Compact gene diagram
RNA-seq gene expression profile across 16 selected tissues from the Non-Human Primates Reference Transcriptome Resource (link to NHPRTR project
- Primates: Apes
: Human (Illumina BodyMap 2), CHP
: Chimpanzee), Old World monkeys
: Pig-Tailed Macaque, JMI
Japanese Macaque, RMI
Rhesus Macaque Indian, RMC
Rhesus Macaque Chinese, CMM
Cynomolgus Macaque Mauritian, CMC
Cynomolgus Macaque Chinese, BAB
Olive Baboon, SMY
Sooty Mangabey); New World monkeys
common Marmoset, SQM
Squirrel Monkey, OWL
Owl Monkey); and Lemurs
Mouse Lemur, RTL
- The level for significantly expressed genes is color coded in 8 equal sized bins (light to dark green). Light gray is for weak not-accurately measured expression (2 to 8 reads above intergenic background); dark gray for no expression or no sequence conservation (0 read in gene). The plot to the right shows the distribution of measured expression values in all tissues for all genes (blue)
and for this gene (green)
, in Magic index = log2
You may also examine the strand-specific genome coverage plots on the experimental AceView/Magic hub at UCSC
, by tissue or by species. Tracks may be slow to load
; please reload if some tracks come up yellow-greenish, and thanks to UCSC for the great work!.
About UCSC tracks: you may enjoy the plots for the summed coverage over all primates' libraries (top track), summarizing 3 terabases of stranded RNA-seq. Fragments mapping on the + strand of the genome (from genes on the + strand) are red (or dark), on minus strand blue (or light) and antisense transcribed areas are black or overlaid. The vertical scale for each track is self-adapting. Homozygous SNPs tracks are also presented.
About mapping: Primates body map RNA-seq data were stringently mapped to the human genome using the NCBI Magic pipeline. Normalized results are shown as significant FPKM (sFPKM), which includes corrections on F, K and M, computed from parameters measured directly in each RNA-seq experiment, to render the expression measures more significant and more robust to experimental biases. Only fragments with both reads mapped uniquely and over at least 80+80 bases ending with 8 exact bases on each side of each read, and facing each other in a single site or gene, are included in the computation of the sFPKM/index, in the coverage plots, and in the determination of homozygous SNPs (minimum coverage 10, minimum allele frequency 95%). But be aware that genes whose sequence evolved to become too distant from Human cannot be measured well, this bias can be appreciated in the per-species coverage plots at UCSC.
About libraries: For non-human primates, total RNA libraries used TruSeq, ribozero and the stranded UDG protocol. The human 2010 libraries used the polyA selected non-stranded protocol, with short reads (50, 75 or 50+50 bases); furthermore the insert lengths are larger in human than in the non-human primates (average insert size 187 bp in non-human primates versus 232 bp in human). These protocol differences may impact expression measures for the non polyadenylated genes (or genes with shorter or occasional polyA tails), for the pseudogenes or close gene families (specificity is reduced in humans due to shorter reads), and for the very short genes.
Sequences: click on the numbers to get the DNA
Gene neighbors and Navigator on chromosome 5q34
Annotated mRNA diagrams
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line
denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line
denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink
and ] - ] blue
straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags
to any single letter variant of the main . More explanations are given in the gene help file
Bibliography:   84 articles in PubMed
The mRNAs diagrams with the aligned cDNA sequence accessions and their mismatches are available in the mRNA pages accessible from the tab at the top of the page, or here:
In Flash: .c, .d-u, .b, .a, .f, .g, .e
or in GIF: .c, .d-u, .b, .a, .f, .g, .e
|? ||Gene Summary
||Gene on genome
||mRNA:.a, .b, .c, .d-u, .e, .f, .g
||Alternative mRNAs features, proteins, introns, exons, sequences
||Function, regulation, related genes DCI
To mine knowledge
about the gene, please click the 'Gene Summary'
or the 'Function, regulation, related genes '
tab at the top of the page. The 'Gene Summary'
page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes '
page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To compare alternative variants
, their summarized annotations, predicted proteins, introns and exons, or to access any sequence, click the 'Alternative mRNAs features'
tab. To see a specific mRNA variant
diagram, sequence and annotation, click the variant name in the 'mRNA'
tab. To examine expression data
from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'
If you know more about this gene, or found errors, please share
your knowledge. Thank you !