AceView: Gene:ZCWPW2, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView.

Expression: According to AceView, this gene is well expressed, 0.4 times the average gene in this release. The sequence of this gene is defined by 51 GenBank accessions from 47 cDNA clones, some from placenta cot 25-normalized (seen 5 times), islets of langerhans (4), pancreas (4), testis (4), brain (3), kidney (2), melanocyte (2) and 20 other tissues. We annotate structural defects or features in 2 cDNA clones.
Alternative mRNA variants and regulation: The gene contains 20 distinct gt-ag introns. Transcription produces 7 different mRNAs, 5 alternatively spliced variants and 2 unspliced forms. There are 3 non overlapping alternative last exons and 4 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 5 cassette exons. 702 bp of this gene are antisense to spliced gene AZI2, raising the possibility of regulated alternate expression.
Function: There are 2 articles specifically referring to this gene in PubMed. Proteins are expected to have molecular functions (metal ion binding, zinc ion binding) and to localize in various compartments (cytoplasm, nucleus). No phenotype has yet been reported to our knowledge: this gene's in vivo function is yet unknown.
Protein coding potential: 4 spliced and 2 unspliced mRNAs putatively encode good proteins, altogether 6 different isoforms (3 complete, 2 COOH complete, 1 partial), some containing domains PWWP domain, CW-type Zinc Finger [Pfam], a coiled coil stretch [Psort2]. The remaining mRNA variant (spliced) appears not to encode a good protein.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
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cDNA accession Links to the sequence	Tissue most 5' clones in red	Match mRNA	From bp to bp in mRNA	From bp to bp in accs.	Clone encodes complete protein (with AA variation)	Features	Anomalies detected by AceView	Accession match over (% length)	Base differences relative to genome (% identity)
AW393564	stomach	.a	815 to 1383	589 to 21				569/589 (96 %)	0 diff (100 %id)
BC094696	Muscle pool- 2 tissues- cardiac and skeletalmuscle.	.a	21 to 1710	1 to 1690	exact	tiling clone, AAA		1690/1690 (100 %)	0 diff (100 %id)
BE144884	head_neck	.f-u	1070 to 1570	1 to 502		tiling clone,		502/502 (100 %)	9 diff (98.3 %id)
BG183206		.a	849 to 1483	4 to 640				637/846 (75 %)	41 diff (95.2 %id)
BG197719		.b	433 to 1178	1 to 746		tiling clone,		746/747 (99 %)	13 diff (98.3 %id)
BG199790		.b	435 to 1085	1 to 651				651/788 (82 %)	20 diff (97.5 %id)
BI037904	nervous_tumor	.a	845 to 1292	31 to 477				447/477 (93 %)	0 diff (100 %id)
BX118914	pooled	.c	1 to 467	1 to 468		tiling clone,		468/468 (100 %)	1 diff (99.8 %id)
CN311971	embryonic stem cells, cell lines H1, H7, andH9	.a	182 to 684	1 to 502				502/507 (99 %)	5 diff (99.1 %id)
BX356878	Placenta Cot 25-normalized	.f-u	630 to 1550	930 to 26	A2C V3L S4X V5X F6X ...	tiling clone,		905/931 (97 %)	0 diff (100 %id)
BX371618	Placenta Cot 25-normalized	.f-u	5 to 891	890 to 1		tiling clone,		890/894 (99 %)	42 diff (95.4 %id)
BX371619	Placenta Cot 25-normalized	.f-u	1 to 910	910 to 1		tiling clone,		910/910 (100 %)	0 diff (100 %id)
BX378320	Placenta Cot 25-normalized	.d-u	1 to 1043	23 to 1049	K30N P31Q G32V S33R V34S ...	tiling clone,		1035/1092 (94 %)	18 diff (98.4 %id)
BX390360	Placenta Cot 25-normalized	.d-u	806 to 1719	1 to 906		tiling clone,		906/916 (98 %)	44 diff (95.2 %id)
AL599988		.d-u	686 to 1307	1 to 622				622/622 (100 %)	0 diff (100 %id)
DB449916	testis	.a	175 to 590	42 to 451				410/451 (90 %)	3 diff (99.4 %id)
DB515266	testis	.a	813 to 1236	1 to 424			Submitted on the opposite strand	424/424 (100 %)	0 diff (100 %id)
DB451817	testis	.a	1 to 497	1 to 497		tiling clone,		497/497 (100 %)	0 diff (100 %id)
DB517325	testis	.a	771 to 1236	1 to 466			Submitted on the opposite strand	466/466 (100 %)	1 diff (99.8 %id)
H97942	melanocyte	.f-u	1406 to 2023	614 to 2		tiling clone,		613/617 (99 %)	0 diff (100 %id)
N72175	melanocyte	.f-u	1768 to 2023	258 to 2				257/258 (99 %)	0 diff (100 %id)
W00455	melanocyte	.f-u	199 to 641	1 to 445				445/445 (100 %)	6 diff (98.7 %id)
AA604713		.f-u	1600 to 2028	8 to 436		tiling clone,		429/436 (98 %)	0 diff (100 %id)
AI553945	pooled	.e	1 to 427	429 to 3	exact	tiling clone,		427/429 (99 %)	0 diff (100 %id)
AI808897	pooled	.c	281 to 759	482 to 3		tiling clone,		480/482 (99 %)	1 diff (99.8 %id)
AW027938	Kidney	.b	757 to 1178	425 to 3				423/425 (99 %)	0 diff (100 %id)
AW593058	pooled	.a	73 to 474	402 to 1				402/402 (100 %)	0 diff (100 %id)
CB047283	Kidney	.d-u	1342 to 1988	674 to 28		tiling clone, AAA		647/647 (100 %)	1 diff (99.9 %id)
CB047284	Kidney	.d-u	8 to 427	10 to 429		tiling clone,		420/429 (97 %)	0 diff (100 %id)
BF575233	muscle (skeletal)	.a	729 to 1474	1 to 741				741/755 (98 %)	33 diff (95.7 %id)
BC065764	Lung, mucoepidermoid carcinoma	.b	1 to 1176	4 to 1179	L86Q	tiling clone, available from MGC, AAA		1176/1176 (100 %)	5 diff (99.6 %id)
BG496727	lung, mucoepidermoid carcinoma	.b	1 to 667	1 to 671		available from MGC,		671/671 (100 %)	11 diff (98.4 %id)
BI091389	cervix	.f-u	204 to 971	1 to 767		tiling clone,		767/803 (95 %)	9 diff (98.9 %id)
BI520920	brain, medulla	.g	422 to 1231	828 to 23	D2K K3R E4K K5M L6D ...	tiling clone,		806/883 (91 %)	12 diff (98.7 %id)
BM908208	brain, medulla	.g	1 to 847	14 to 869		tiling clone,	Suspected internal deletion	852/1106 (77 %)	10 diff (99.1 %id)
BQ086331	Islets of Langerhans, Pancreas	.a	328 to 901	1 to 574				574/574 (100 %)	1 diff (99.9 %id)
BQ102408	Islets of Langerhans, Pancreas	.b	616 to 1125	521 to 12		AAA		510/510 (100 %)	1 diff (99.9 %id)
CK822989	Islets of Langerhans, Pancreas	.b	542 to 1125	595 to 12		tiling clone, AAA		584/584 (100 %)	1 diff (99.9 %id)
CK822990	Islets of Langerhans, Pancreas	.a	328 to 928	1 to 601				601/601 (100 %)	1 diff (99.9 %id)
BU508346	leiomyosarcoma, uterus	.b	1131 to 2019	1 to 889		tiling clone,		889/889 (100 %)	2 diff (99.8 %id)
CF552461	Pooled muscle (cardiac and skeletal)	.a	21 to 875	30 to 884				855/884 (96 %)	0 diff (100 %id)
TI_43963384		.g	422 to 1231	828 to 23	D2K K3R E4K K5M L6D ...			806/976 (82 %)	12 diff (98.8 %id)
NM_001040432.1		.a	47 to 1710	1 to 1664	exact	RefSeq, AAA		1664/1664 (100 %)	0 diff (100 %id)
DN991282	brain, whole brain	.a	174 to 759	17 to 602				586/602 (97 %)	0 diff (100 %id)
DB288017	uterus	.d-u	1 to 563	2 to 565		capped		564/564 (100 %)	3 diff (99.5 %id)
DA246920	brain	.a	328 to 928	1 to 601		capped		601/601 (100 %)	1 diff (99.9 %id)
DB306158	brain	.b	1131 to 2019	1 to 889		tiling clone,		889/889 (100 %)	2 diff (99.8 %id)
AL118588	amygdala	.b	1131 to 2019	1 to 889		tiling clone,	Suspected internal deletion, this clone was ignored	889/889 (100 %)	2 diff (99.8 %id)
FN083369	breast carcinoma	.a	21 to 875	30 to 884				855/884 (96 %)	0 diff (100 %id)
AI678197	Prostate	.g	422 to 1231	828 to 23				806/976 (82 %)	12 diff (98.8 %id)
AI677721	pooled	.a	47 to 1710	1 to 1664		RefSeq, AAA		1664/1664 (100 %)	0 diff (100 %id)
DN991282	brain, whole brain	.a	174 to 759	17 to 602				586/602 (97 %)	0 diff (100 %id)
DN991282	brain, whole brain	.a	174 to 759	17 to 602				586/602 (97 %)	0 diff (100 %id)
DB288017	uterus	.d-u	1 to 563	2 to 565		capped		564/564 (100 %)	3 diff (99.5 %id)