AceView: Gene:TTC18, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView.

Expression: According to AceView, this gene is well expressed, 0.7 times the average gene in this release. The sequence of this gene is defined by 80 GenBank accessions from 71 cDNA clones, some from lung (seen 11 times), testis (10), nervous normal (5), brain (2), breast carcinoma (2), carcinoid (2), germinal center B cell (2) and 21 other tissues. We annotate structural defects or features in 6 cDNA clones.
Alternative mRNA variants and regulation: The gene contains 15 distinct gt-ag introns. Transcription produces 6 different mRNAs, 5 alternatively spliced variants and 1 unspliced form. There are 3 probable alternative promotors, 2 non overlapping alternative last exons and 3 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 3 cassette exons, overlapping exons with different boundaries. 331 bp of this gene are antisense to spliced gene kleynoby, 542 to skarma, raising the possibility of regulated alternate expression.
Note that mRNA .eAug10 was found in vivo, although it is a predicted target of nonsense mediated mRNA decay (NMD).
Function: There is one article specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to a disease (Alzheimer Disease). Proteins are expected to have molecular function (binding) and to localize in cytoplasm.
Protein coding potential: 5 spliced mRNAs putatively encode good proteins, altogether 5 different isoforms (2 complete, 2 COOH complete, 1 partial), some containing tetratricopeptide repeat domain [Pfam], a coiled coil stretch [Psort2]. The remaining mRNA variant (unspliced) appears not to encode a good protein.
2 isoforms are annotated using as Met a Kozak-compatible non-AUG start, thereby gaining a minimum of 87 amino acids N-terminal to the first AUG.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
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cDNA accession Links to the sequence	Tissue most 5' clones in red	Match mRNA	From bp to bp in mRNA	From bp to bp in accs.	Clone encodes complete protein (with AA variation)	Features	Anomalies detected by AceView	Accession match over (% length)	Base differences relative to genome (% identity)
AF435958		.d	1 to 1231	1 to 1224	L34*	tiling clone,		1226/1229 (99 %)	11 diff (99.2 %id)
AF435959		.c	878 to 2198	1 to 1314	L34*	tiling clone,		1316/1319 (99 %)	11 diff (99.2 %id)
AK098631	testis	.a	1 to 1706	1 to 1706	exact	tiling clone, available from FLJ,		1706/1706 (100 %)	1 diff (100.0 %id)
BF895534	marrow	.a	965 to 1208	269 to 26			Submitted on the opposite strand	244/269 (90 %)	1 diff (99.7 %id)
BF895539	marrow	.a	870 to 1208	364 to 24		tiling clone,	Submitted on the opposite strand	341/364 (93 %)	1 diff (99.8 %id)
BF942801	nervous_normal	.c	22 to 595	598 to 28		tiling clone,	Submitted on the opposite strand	571/600 (95 %)	0 diff (100 %id)
BF942805	nervous_normal	.c	49 to 619	569 to 2		tiling clone,	Submitted on the opposite strand	568/571 (99 %)	0 diff (100 %id)
BG192583		.c	1899 to 2194	1 to 294		AAA		294/295 (99 %)	7 diff (97.7 %id)
BG196633		.c	1919 to 2194	1 to 276		AAA		276/276 (100 %)	7 diff (97.5 %id)
BM422374	testis	.c	1899 to 2193	6 to 300				295/314 (93 %)	6 diff (98.1 %id)
BX093691	lung	.c	1411 to 2194	1 to 784		AAA		784/784 (100 %)	0 diff (100 %id)
BX281792	testis	.c	1769 to 2194	454 to 29		AAA	Submitted on the opposite strand	426/426 (100 %)	0 diff (100 %id)
C20668		.c	1937 to 2194	1 to 258				258/258 (100 %)	0 diff (100 %id)
CV399792	placenta_normal	.c	243 to 486	193 to 436				244/436 (55 %)	6 diff (98.7 %id)
CV401333	nervous_normal	.c	122 to 624	527 to 26			Submitted on the opposite strand	502/527 (95 %)	0 diff (100 %id)
CV401335	nervous_normal	.c	1 to 367	29 to 400		tiling clone,		372/400 (93 %)	6 diff (98.5 %id)
CV401365	nervous_normal	.c	76 to 437	1 to 365				365/365 (100 %)	18 diff (95.1 %id)
AL043737	testis	.c	1542 to 2166	625 to 1				625/625 (100 %)	0 diff (100 %id)
AL039443	testis	.c	1828 to 2195	1 to 368		fully sequenced, AAA		368/368 (100 %)	3 diff (99.2 %id)
AL039444	testis	.c	1866 to 2194	372 to 44		fully sequenced, AAA		329/329 (100 %)	1 diff (99.7 %id)
FN098294	breast carcinoma	.f-u	88 to 195	1 to 108				108/109 (99 %)	2 diff (98.2 %id)
FN117556	breast carcinoma	.a	589 to 658	69 to 1			Submitted on the opposite strand	69/70 (98 %)	1 diff (98.6 %id)
DB508426	testis	.c	1785 to 2194	1 to 409		AAA	Submitted on the opposite strand	409/409 (100 %)	1 diff (99.8 %id)
DB512061	testis	.c	1781 to 2194	1 to 413			Submitted on the opposite strand	413/413 (100 %)	6 diff (98.6 %id)
DB520200	testis	.c	1742 to 2192	1 to 451			Submitted on the opposite strand	451/453 (99 %)	1 diff (99.8 %id)
N93317	lung	.c	1724 to 2194	472 to 1		fully sequenced,		472/472 (100 %)	1 diff (99.8 %id)
W25140	lung	.c	1410 to 1784	1 to 381		fully sequenced,		381/381 (100 %)	10 diff (97.4 %id)
AA678408	liver and spleen	.c	1776 to 2189	414 to 1				414/414 (100 %)	1 diff (99.8 %id)
AA449760		.c	1801 to 2194	412 to 21		AAA		392/392 (100 %)	2 diff (99.5 %id)
AA461091		.c	1826 to 2194	387 to 21		fully sequenced, AAA		367/367 (100 %)	1 diff (99.8 %id)
AA461405		.c	1687 to 1887	1 to 201		fully sequenced,		201/201 (100 %)	0 diff (100 %id)
AA767809	germinal center B cell	.f-u	100 to 472	420 to 41	P41L F44Y			380/420 (90 %)	0 diff (100 %id)
AA769128	germinal center B cell	.f-u	1 to 509	509 to 1	exact	tiling clone,		509/509 (100 %)	0 diff (100 %id)
AI027249		.c	1782 to 2188	422 to 16		AAA		407/407 (100 %)	0 diff (100 %id)
AI204701	heart	.f-u	172 to 508	337 to 1	exact			337/337 (100 %)	0 diff (100 %id)
AI187125	lung	.c	1713 to 2194	482 to 1				482/482 (100 %)	0 diff (100 %id)
AI208344		.c	1868 to 2198	332 to 2				331/332 (99 %)	0 diff (100 %id)
AI283543	pooled	.c	1770 to 2197	427 to 1				427/427 (100 %)	1 diff (99.8 %id)
AI432299	pooled	.c	1713 to 2192	481 to 2				480/481 (99 %)	0 diff (100 %id)
AI458159	carcinoid, lung	.c	1754 to 2201	458 to 12		tiling clone, AAA		447/456 (98 %)	2 diff (99.6 %id)
AI538905	poorly-differentiated endometrialadenocarcinoma, 2 pooled tumors, uterus	.c	1831 to 2200	370 to 1		tiling clone,		370/370 (100 %)	0 diff (100 %id)
AI684461	pooled	.c	1675 to 2194	520 to 1				520/520 (100 %)	0 diff (100 %id)
AI808236	pooled	.c	1949 to 2191	243 to 1				243/243 (100 %)	2 diff (99.2 %id)
AW024437	Kidney	.a	1132 to 1706	577 to 1				577/577 (100 %)	3 diff (99.5 %id)
AW150097	uterus, well-differentiated endometrialadenocarcinoma, 7 pooled tumors	.c	1742 to 2198	462 to 6				457/462 (98 %)	0 diff (100 %id)
AW241880	pooled	.c	1757 to 2179	424 to 1				424/424 (100 %)	5 diff (98.9 %id)
AW444635		.c	1754 to 2194	458 to 18		AAA		441/451 (97 %)	1 diff (99.8 %id)
AW300070	Kidney	.a	1188 to 1690	505 to 3				503/505 (99 %)	0 diff (100 %id)
AW594330	pooled germ cell tumors	.c	1770 to 2192	432 to 6				346/432 (80 %)	12 diff (97.3 %id)
AW613917	pooled germ cell tumors	.c	1754 to 2171	420 to 1				420/430 (97 %)	2 diff (99.6 %id)
AW612223	carcinoid, lung	.c	1824 to 2179	356 to 1				356/356 (100 %)	0 diff (100 %id)
BI831350	brain, medulla	.d	1 to 826	1 to 824		tiling clone,		826/864 (95 %)	20 diff (97.7 %id)
BQ019201	lung, Metastatic Chondrosarcoma	.c	1537 to 2194	676 to 19		AAA		658/658 (100 %)	0 diff (100 %id)
CD107987	brain, Pituitary	.c	170 to 1023	1 to 859		tiling clone,		859/864 (99 %)	10 diff (98.9 %id)
CD244513	testis	.a	128 to 913	1 to 794				792/840 (94 %)	18 diff (97.9 %id)
CF456645	peripheral nervous system	.e	1 to 749	32 to 780	A63V	tiling clone,		749/780 (96 %)	7 diff (99.2 %id)
TI_57172097		.d	1 to 826	67 to 890				826/930 (88 %)	20 diff (97.9 %id)
TI_1948149573		.c	1226 to 1984	888 to 130				759/1232 (61 %)	12 diff (99.1 %id)
TI_1948150161		.b	125 to 1040	1045 to 128	T2Y K3Q Q4R K5Q S6K ...	tiling clone,		1075/1210 (88 %)	42 diff (96.6 %id)
TI_1948150162		.c	1067 to 1984	1058 to 137		tiling clone,		922/1275 (72 %)	15 diff (98.9 %id)
TI_1948150468		.c	1090 to 1984	1025 to 128				898/1281 (70 %)	31 diff (97.6 %id)
TI_1948150469		.c	1083 to 1984	1037 to 135		tiling clone,		903/1237 (72 %)	16 diff (98.8 %id)
TI_149298416		.c	1663 to 2194	613 to 82		AAA		532/766 (69 %)	1 diff (99.9 %id)
TI_149305776		.a	1039 to 1706	744 to 79		AAA		666/910 (73 %)	3 diff (99.7 %id)
TI_149308447		.c	1560 to 2194	723 to 88		AAA		636/975 (65 %)	8 diff (99.2 %id)
TI_158431263		.c	1538 to 2171	732 to 95		AAA		638/867 (73 %)	8 diff (99.1 %id)
TI_159723686		.c	1250 to 2194	1006 to 80		AAA		927/974 (95 %)	27 diff (97.3 %id)
TI_159723759		.c	1229 to 2194	1025 to 80		AAA		946/983 (96 %)	38 diff (96.2 %id)
CF528535	placenta	.c	1586 to 2171	600 to 15		AAA		586/586 (100 %)	0 diff (100 %id)
BU686075	lung, Primary Lung Epithelial Cells	.a	1059 to 1706	665 to 19		tiling clone, AAA		647/647 (100 %)	2 diff (99.7 %id)
BM979653	lung, Primary Lung Epithelial Cells	.c	1595 to 2194	619 to 19		AAA		601/601 (100 %)	7 diff (98.9 %id)
BM983163	lung, Primary Lung Cystic Fibrosis EpithelialCells	.c	1687 to 2194	526 to 19		AAA		508/508 (100 %)	0 diff (100 %id)
CB853554	Human Lung Epithelial cells, lung	.c	1531 to 2194	682 to 19		AAA		664/664 (100 %)	6 diff (99.1 %id)
CB853556	Human Lung Epithelial cells, lung	.c	1531 to 2194	682 to 19		AAA		664/664 (100 %)	0 diff (100 %id)
AK308746. matches multiple genes	amygdala	.c	1229 to 2194	1025 to 80			This clone was ignored, also hits gene nakimi, porsmor	946/983 (96 %)	38 diff (96.2 %id)
AK308746. matches multiple genes	amygdala	.c	1586 to 2171	600 to 15			This clone was ignored, also hits gene nakimi, porsmor	586/586 (100 %)	0 diff (100 %id)
AK308746. matches multiple genes	amygdala	.a	1059 to 1706	665 to 19		tiling clone,	This clone was ignored, also hits gene nakimi, porsmor	647/647 (100 %)	2 diff (99.7 %id)
AL833537. matches multiple genes	cervix	.a	1059 to 1706	665 to 19		tiling clone, available from DKFZ, AAA	This clone was ignored, also hits gene nakimi	647/647 (100 %)	2 diff (99.7 %id)
AL833537. matches multiple genes	cervix	.c	1595 to 2194	619 to 19		available from DKFZ, AAA	This clone was ignored, also hits gene nakimi	601/601 (100 %)	7 diff (98.9 %id)
BC032856. matches multiple genes	testis	.c	1595 to 2194	619 to 19		available from MGC, AAA	This clone was ignored, also hits gene nakimi	601/601 (100 %)	7 diff (98.9 %id)
BC032856. matches multiple genes	testis	.c	1687 to 2194	526 to 19		available from MGC, AAA	This clone was ignored, also hits gene nakimi	508/508 (100 %)	0 diff (100 %id)
NM_145170.3. matches multiple genes		.c	1687 to 2194	526 to 19		RefSeq, AAA	This clone was ignored, also hits gene nakimi	508/508 (100 %)	0 diff (100 %id)
NM_145170.3. matches multiple genes		.c	1531 to 2194	682 to 19		RefSeq, AAA	This clone was ignored, also hits gene nakimi	664/664 (100 %)	6 diff (99.1 %id)
AV751223	Pituitary	.c	1531 to 2194	682 to 19			This clone was ignored	664/664 (100 %)	6 diff (99.1 %id)
TI_1948149863		.c	1531 to 2194	682 to 19			Suspected internal deletion, this clone was ignored	664/664 (100 %)	0 diff (100 %id)
TI_1948149864		.c	1531 to 2194	682 to 19			Suspected internal deletion, this clone was ignored	664/664 (100 %)	0 diff (100 %id)