Note that this locus is complex: it appears to produce several proteins with no sequence overlap.
Expression: According to AceView, this gene is expressed at very high level, 5.8 times the average gene in this release. The sequence of this gene is defined by 1240 GenBank accessions, some from brain (seen 128 times), hippocampus (53), amygdala (48), uterus (47), breast carcinoma (33), nervous tumor (20), eye (18) and 106 other tissues.
Alternative mRNA variants and regulation: The gene contains 31 distinct introns (30 gt-ag, 1 gc-ag). Transcription produces 22 different mRNAs, 20 alternatively spliced variants and 2 unspliced forms. There are 3 probable alternative promotors, 4 non overlapping alternative last exons and 7 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 21 cassette exons, overlapping exons with different boundaries. 199 bp of this gene are antisense to spliced gene NUDT9, raising the possibility of regulated alternate expression.
Note that mRNA .mAug10 was found in vivo, although it is a predicted target of nonsense mediated mRNA decay (NMD).
Efficacy of translation may be reduced by the presence of a shorter translated product (uORF) initiating at an AUG upstream of the main open reading frame (in variant bAug10, cAug10, dAug10, eAug10, gAug10, hAug10).
Function: There are 14 articles specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to diseases (Cell Transformation, Neoplastic; Neoplasms) and proposed to participate in a process (signal transduction). Proteins are expected to have molecular function (calcium ion binding) and to localize in various compartments (extracellular space, extracellular region, proteinaceous extracellular matrix, synapse). Putative protein interactors have been described (PRNP, SKIL).
Protein coding potential: 14 spliced mRNAs putatively encode good proteins, altogether 13 different isoforms (4 complete, 1 COOH complete, 8 partial), some containing domains Follistatin/Osteonectin-like EGF domain, Kazal-type serine protease inhibitor domain, Kazal-type serine protease inhibitor domain, Secreted protein acidic and rich in cysteine Ca binding region [Pfam], a coiled coil stretch [Psort2]; 2 of the 4 complete proteins appear to be secreted. The remaining 8 mRNA variants (6 spliced, 2 unspliced; 5 partial) appear not to encode good proteins.
2 isoforms are annotated using as Met a Kozak-compatible non-AUG start, thereby gaining a minimum of 64 amino acids N-terminal to the first AUG. Finally proteins from this gene may be modulated by phosphorylation, as detailed at PhosphoSite.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Read more...