Note that this locus is complex: it appears to produce several proteins with no sequence overlap.
Expression: According to AceView, this gene is expressed at high level, 2.9 times the average gene in this release. The sequence of this gene is defined by 325 GenBank accessions from 311 cDNA clones, some from placenta cot 25-normalized (seen 24 times), breast (20), lung (14), neuroblastoma (12), prostate (10), colon (9), brain (8) and 115 other tissues. We annotate structural defects or features in 4 cDNA clones.
Alternative mRNA variants and regulation: The gene contains 14 distinct introns (12 gt-ag, 2 gc-ag). Transcription produces 11 different mRNAs, 7 alternatively spliced variants and 4 unspliced forms. There are 3 probable alternative promotors, 2 non overlapping alternative last exons and 4 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 3 cassette exons, overlapping exons with different boundaries, splicing versus retention of one intron.
Note that mRNA .eAug10 was found in vivo, although it is a predicted target of nonsense mediated mRNA decay (NMD).
Function: There are 46 articles specifically referring to this gene in PubMed. Functionally, the gene has been proposed to participate in pathway (Proteasome) and processes (anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process, negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle, positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle). Proteins are expected to have molecular functions (ATPase activity, protein binding, ATP binding, hydrolase activity, nucleotide binding) and to localize in various compartments (cytoplasm, nucleolus, nucleus, proteasome complex). Putative protein interactors have been described (ATXN7, CCDC85B, PAAF1, PMS1, PMS2, PSMA7, PSMC2, PSMC4, PSMC5, PSMD2 and 10 others).
Protein coding potential: 7 spliced and the unspliced mRNAs putatively encode good proteins, altogether 8 different isoforms (6 complete, 1 COOH complete, 1 partial), some containing domains ATPase family associated with various cellular activities (AAA), ATPase family associated with various cellular activities (AAA) [Pfam], a N-myristoylation domain [Psort2]. The remaining 3 mRNA variants (3 unspliced; 1 partial) appear not to encode good proteins. Finally proteins from this gene may be modulated by acetylation; phosphorylation; ubiquitination, as detailed at PhosphoSite.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
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