Note that this locus is complex: it appears to produce several proteins with no sequence overlap.
Expression: According to AceView, this gene is expressed at high level, 2.0 times the average gene in this release. The sequence of this gene is defined by 236 GenBank accessions from 211 cDNA clones, some from epidermis (seen 13 times), eye (11), brain (7), colon (7), liver (7), prostate (7), nervous normal (6) and 95 other tissues. We annotate structural defects or features in 28 cDNA clones.
Alternative mRNA variants and regulation: The gene contains 28 distinct introns (25 gt-ag, 2 gc-ag, 1 other). Transcription produces 12 different mRNAs, 10 alternatively spliced variants and 2 unspliced forms. There are 6 probable alternative promotors, 5 non overlapping alternative last exons and 4 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 3 cassette exons, overlapping exons with different boundaries, splicing versus retention of 4 introns. 714 bp of this gene are antisense to spliced gene shosmer, raising the possibility of regulated alternate expression.
Note that mRNA .cAug10 was found in vivo, although it is a predicted target of nonsense mediated mRNA decay (NMD).
Efficacy of translation may be reduced by the presence of a shorter translated product (uORF) initiating at an AUG upstream of the main open reading frame (in variant eAug10).
Function: There are 18 articles specifically referring to this gene in PubMed. Functionally, the gene has been proposed to participate in processes (translational elongation, protein amino acid phosphorylation). Proteins are expected to have molecular functions (protein kinase activity, translation factor activity, nucleic acid binding, ATP binding, calcium ion binding and 5 others) and to localize in various compartments (cytoplasm, cytosol). Putative protein interactors have been described (MAPK8, MAPK9, MAPK11, MAPK12ANDHDAC10, MAPK13, MAPK14, MAPKAPK2, MAPKAPK3, MAPKAPK5, PRKAA1, PRKAA2, PRKACA, RPS6KA1, RPS6KB1, RPS6KB2).
Protein coding potential: 7 spliced mRNAs putatively encode good proteins, altogether 6 different isoforms (4 complete, 1 COOH complete, 1 partial), some containing domains Alpha-kinase family, Sel1 repeat [Pfam]. The remaining 5 mRNA variants (3 spliced, 2 unspliced; 2 partial) appear not to encode good proteins. Finally proteins from this gene may be modulated by phosphorylation; ubiquitination, as detailed at PhosphoSite.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12

Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
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