[Home] [ICTV Taxonomy - Index of Viruses] [Virus Descriptions] [Character List] [Picture Gallery]
[Tutorial] [Online Data Retrieval & Identification] [Virus Isolate Registration & Submission] [Search]
Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

01.062.0.04.004. Festuca leaf streak virus

Cite this publication as: ICTVdB Management (2006). 01.062.0.04.004. Festuca leaf streak virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Denmark.

Host of Isolate and Habitat Details Natural hosts and symptoms

Festuca gigantea — chlorotic streaks of some veins of mature leaves.

Reference to Isolation Report
Lundsgaard and Albrechtsen (1976).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 01.062.0.04.004. Virus accession number: 62004004. Obsolete virus code: 62.0.4.0.004; superceded accession number: 62040004.

Name, Synonyms and Lineage

ICTV approved acronym: FLSV. Virus is an ICTV approved species of the genus Cytorhabdovirus in the family 01.062. Rhabdoviridae; order 01. Mononegavirales.

Virion Properties

Morphology

Virions consist of an envelope and a nucleocapsid. Virus capsid is enveloped. Virions are bullet-shaped. Virions measure 61 nm in diameter; 286 nm in length. Capsid/nucleocapsid is elongated with helical symmetry. The nucleocapsid is uncoiled filamentous, or cylindrical (coiled, is cross-striated and a width of 36 nm. Axial canal is distinct; 15 nm in diameter. Basic helix is obvious. Pitch of helix is 3.7 nm.
Electron micrograph of Festuca leaf streak virus by Thorben Lundsgaard.

Electron microscopic preparation and references: Virus preparation contains few virions. Virions are disrupted when extracted in 2% PTA. Virions are preserved by 2% AM extraction or aldehyde fixation prior to PTA staining. Reference for electron microscopic methods: Lundsgaard (1987).

Physicochemical and Physical Properties

Virions have a buoyant density in sucrose of 1.194 g cm-3. There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 704 S20w.

Nucleic Acid

The genome is not segmented and contains a single molecule of linear single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain nucleic acid of host origin including probably host rRNA. The complete genome is 14000 nucleotides long. The RNA is sequenced, but only an estimate is available, complete sequence is 14000 nucleotides long. Reference to nucleotide sequence Lundsgaard (1987).

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 3 structural protein(s) located in the envelope, peplomers, and nucleocapsid.

Structural Proteins: Envelope protein G that forms the surface peplomers is the largest; has a molecular mass of 58000 Da. Envelope protein has a function assigned; is an attachment protein and a fusion protein. Envelope protein M binding to nucleocapsid and the cytoplasmic domain of G; has a molecular mass of 20000 Da. Capsid protein N. Nucleocapsid protein has a molecular mass of 49000 Da.

Reference to method of preparation: Lundsgaard and Albrechtsen (1976).

Lipids

Lipids are present and located in the envelope. The lipids are of host origin.

Replication cycle Viral proteins accumulate in the cytoplasm; are associated with the endoplasmic reticulum, cisternae of endoplasmic reticulum, and nuclear envelope. Virions accumulate in the cell cytoplasm.

Maturation: Virions mature by budding through plasma membranes of the endoplasmic reticulum.

Release: The virus envelope is acquired from the host cell by budding. The virus envelope is drived from the endoplasmic reticulum.

Antigenicity

The virus does not show serological relationships to Northern cereal mosaic virus, Barley yellow striate mosaic virus, Cereal chlorotic mottle virus, Maize sterile stunt virus, Digitaria striate virus and Moroccan wheat virus (Lundsgaard, 1984; Greber, 1990). Antigenic specificity of the virion can be determined by agar gel-diffusion tests, ELISA tests, and immune electron microscopy assays.

Diagnostics and Reference Collections

The best tests for diagnosis are serology.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

Class Liliopsida (Monocotyledonae)
Subclass COMMELINIDAE; Order Poales;
Family Poaceae. Virus found in Festuca gigantea.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is not transmitted by mechanical inoculation; not transmitted by seeds.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Hemiptera, family Delphacidae; Javesella pellucida. Virus is not transmitted by Javesella discolor, J. dubia, Laodelphax striatellus, Muellerianella brevipennis, Psammotettix alienus.

Experimental Hosts and Symptoms

Susceptible host species are found in the Family Gramineae. The following species were susceptible to experimental virus infection: Avena sativa, Hordeum vulgare, and Triticum aestivum.

Host:
Experimental host is susceptible to infection Avena sativa. Experimentally infected hosts mainly show symptoms of chlorotic leaf streaks.

Host 2: Experimental host is susceptible to infection Hordeum vulgare. Experimentally infected hosts mainly show symptoms of chlorotic leaf streaks.

Host 3: Experimental host is susceptible to infection Triticum aestivum. Experimentally infected hosts mainly show symptoms of chlorotic leaf streaks.

Diagnostic Hosts

Virus has been propagated in protoplast infection of Vigna unguiculata.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Festuca gigantea.

References to host data: Lundsgaard, 1999.

Histopathology: Virus can be best detected in leaves roots, mesophyll and epidermis. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are membranous bodies and viroplasma.

Geographical Distribution

The virus is found, but with no evidence of proliferation, in Denmark and Germany.

References

Greber, R.S. (1990). Ann. appl. Biol. 116: 259.

Lundsgaard, T. (1984). Intervirology 22: 50.

Lundsgaard, T. (1987). J. gen. Virol. 68: 931.

Lundsgaard, T. (1992). J. Phytopath. 134: 27.

Lundsgaard, T. (1995). J. Phytopath. 143: 479.

Lundsgaard, T. (1999). Z. PflKrankh. PflSchutz 106: 545.

Lundsgaard, T. and Albrechtsen, S.E. (1976). Phytopath. Z. 87: 12.

Lundsgaard, T. and Albrechtsen, S.E. (1979). Phytopath. Z. 94: 112.

Van Beek, N.A.M., Lohuis, D., Dijkstra, J. and Peters, D. (1985). J. gen. Virol. 66: 2485.

The following generic references are cited in the most recent ; .

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 336 by T. Lundsgaard, 1999.

Images

Taxon images: • EM by Thorben Lundsgaard.



Limit search to: Title & Body Title Document Path
Show Reverse Sort

DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

ICTVdB and DELTA related References

Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
Copyright © 2002    International Committee on Taxonomy of Viruses.    All rights reserved.


Additional access points to virus species lists, descriptions and images on the web:

Species catalogue                     iSpecies.org - a species search engine           a species search engine

Google Analytics      Google Analytics: activity view