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00.077.0.01.021. Voandzeia necrotic mosaic virus

Cite this publication as: ICTVdB Management (2006). 00.077.0.01.021. Voandzeia necrotic mosaic virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Cote d'Ivoire.

Host of Isolate and Habitat Details
Source of isolate: Vigna subterranea (syn. Voandzeia subterranea).

Natural host and symptoms
Vigna subterranea — mosaic, necrosis and malformation of leaves, occasionally lethal. Comments on host and host range: an unspecified 82 species in Amaranthaceae, Chenopodiaceae, Cruciferae, Leguminosae, Malvaceae, Passifloraceae and Solanaceae are not infected.

Reference to Isolation Report
Fauquet et al. (1981).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.077.0.01.021. Virus accession number: 77001021. Obsolete virus code: 77.0.1.0.017; superceded accession number: 77010017.

Name, Synonyms and Lineage

ICTV approved acronym: VNMV. Virus is an ICTV approved species of the genus 00.077.0.01. Tymovirus; family 00.077. Tymoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 28 nm. Capsids appear hexagonal in outline. The capsomer arrangement is not obvious.

Electron microscopic preparation and references: Virus preparation contains few virions. Reference for electron microscopic methods: Monsarrat et al. (1984).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.45 g cm-3 (B), or 1.29 g cm-3 (T). There are 2 sedimenting component(s) found in purified preparations. The sedimentation coefficient is of the fastest 113 S20w (B, of the other(s) are 51 S20w (T). Isoelectric point pH is 4.65. A260/A280 ratio is 1.75 (B), or 0.64 (T). The thermal inactivation point (TIP) is at in Vigna subterranea sap 70°C. The longevity in vitro (LIV) is 2 days at 10°C and 10 days (at 4°C or -20°C). Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 2.

Nucleic Acid

The Mr of the genome constitutes 35% of the virion by weight (B). The genome is monopartite. Only one particle size of linear, single-stranded RNA is recovered.

Proteins

Proteins constitute about 65% of the particle weight (B).

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Lipids

Lipids are not reported.

Antigenicity

The virus is serologically related to kennedya yellow mosaic, desmodium yellow mottle, okra mosaic and turnip yellow mosaic viruses. The virus does not show serological relationships to cacao yellow mosaic and clitoria yellow vein viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

General Symptoms in Plants Virus affects the photosynthetic system. Symptoms are expressed in the leaf include necrosis; include necrosis of the leaf. Symptoms in leaves include development of patterns or markings that are visible in the intercostal regions. Intercostal regions show necrosis. Leaves are turning necrotic. Leaves with mosaic have necrotic mosaic.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Possibly transmitted by a vector. Virus is transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seeds.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Chenopodiaceae, Leguminosae-Papilionoideae. The following species were susceptible to experimental virus infection: Chenopodium amaranticolor, Lablab purpureus, Vigna subterranea, Vigna unguiculata, Vigna unguiculata ssp. cylindrica, Vigna unguiculata ssp. sesquipedalis , Vigna unguiculata ssp. unguiculata.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Chenopodiaceae, Cruciferae, Leguminosae, Leguminosae-Papilionoideae, Malvaceae, Passifloraceae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Abelmoschus esculentus, or Amaranthaceae, Arachis hypogaea, or Chenopodiaceae, Cruciferae, Leguminosae, Malvaceae, Nicotiana benthamiana, Nicotiana glutinosa, or Passifloraceae, Phaseolus vulgaris, or Solanaceae.

Diagnostic Hosts

Diagnostic host species and symptoms:

Vigna subterranea — systemic chlorosis, veinal necrosis and leaf malformation.

Lablab purpureus, Vigna unguiculata ssp. unguiculata — chlorotic local lesions, then systemic leaf chlorosis.

Chenopodium amaranticolor — necrotic local lesions; not systemic. Diagnostic host: insusceptible host species Arachis hypogaea, Phaseolus vulgaris, Nicotiana benthamiana, Nicotiana glutinosa, Abelmoschus esculentus.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Vigna subterranea, Lablab purpureus, Vigna unguiculata ssp. unguiculata.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium amaranticolor (L).

References to host data: Monsarrat et al. (1984).

Histopathology: Virus can be best detected in leaves and mesophyll. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are crystals. Inclusions contain mature virions. Other cellular changes include the occurrence of peripheral vesicles in chloroplasts bounded by a double membrane.

Geographical Distribution

The virus occurs in Cote d'Ivoire.

Ecology, Epidemiology and Control

Studies reported by Monsarrat et al. (1984).

References

Fauquet, C., Monsarrat, A and Thouvenel, J.-C. (1981). Abstr. 5th. Congr. Virol., Strasbourg, 1981, p. 273.

Fauquet, C., Monsarrat, A and Thouvenel, J.-C. (1984). CMI/AAB Descr. Pl. Viruses No. 279, 4 pp.

Monsarrat, A, Fauquet, C. and Thouvenel, J.-C. (1984). C.r. hebd. Seanc. Acad. Sci. Paris, Series III299: 53.

The following generic references are cited in the most recent ; .

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 872 by A.A. Brunt and A Monsarrat, 1988.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 279.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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