[Home] [ICTV Taxonomy - Index of Viruses] [Virus Descriptions] [Character List] [Picture Gallery]
[Tutorial] [Online Data Retrieval & Identification] [Virus Isolate Registration & Submission] [Search]
Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

00.077.0.01.013. Okra mosaic virus

Cite this publication as: ICTVdB Management (2006). 00.077.0.01.013. Okra mosaic virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Cote d'Ivoire.

Host of Isolate and Habitat Details
Source of isolate: Abelmoschus esculentus.

Natural host and symptoms
Abelmoschus esculentus, Hibiscus rosa-sinensis — mosaic and vein banding.

Abutilon hirtum — yellow mosaic.

Borreria intricans — interveinal mosaic.

Corchorus olitorius, Hibiscus sabdariffa — vein chlorosis and vein banding.

Malvastrum coromandelianum, Sida acuta, S. rhomboidea — yellow mosaic.

Physalis angulata — symptomless.

Sida linifolia — mosaic and vein banding.

Urena lobata — some star-shaped light green areas.

Reference to Isolation Report
Givord et al. (1972).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.077.0.01.013. Virus accession number: 77001013. Obsolete virus code: 77.0.1.0.010; superceded accession number: 77010010.
NCBI Taxon Identifier NCBI Taxonomy ID: 70822.

Name, Synonyms and Lineage

Synonym(s): virus de la mosaique du Gombo. ICTV approved acronym: OkMV. Virus is an ICTV approved species of the genus 00.077.0.01. Tymovirus; family 00.077. Tymoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 28 nm. Capsids appear round. The capsomer arrangement is clearly visible.

Electron microscopic preparation and references: Virus preparation contains many virions. Reference for electron microscopic methods: Givord and Hirth (1973).

Physicochemical and Physical Properties

There are 2 sedimenting component(s) found in purified preparations. The sedimentation coefficient is of the fastest 106 S20w (B, of the other(s) are 42 S20w (T). A260/A280 ratio is 1.68 (B), or 0.89 (T). The thermal inactivation point (TIP) is at 80°C. The longevity in vitro (LIV) is 7-9 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 6. The infectivity is retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 32% of the virion by weight. The genome is monopartite. Only one particle size of linear, single-stranded RNA is recovered. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA that is mRNA. The genome has a base ratio of 17.2 % guanine; 17.5 % adenine; 39.8 % cytosine; 25.5 % uracil. The 3'-terminus has a subgenomic promoter, a conserved region known as "tymobox". The 3'-terminus has a tRNA-like structure that can be aminoacylated with valine.

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 68% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Reference to amino acid sequence or composition Paul et al. (1980).

Lipids

Lipids are not reported.

Genome Organization and Replication

By itself, genomic nucleic acid is infectious.

Antigenicity

The virus is serologically related to kennedya yellow mosaic, desmodium yellow mottle, clitoria yellow vein and cacao yellow mosaic viruses are closely related, and other tymoviruses are distantly serologically related (Koenig, 1976).

VMG-CI, VMG-Nig and VM.HR can be distinguished by five diagnostic species and by their serological interrelationships (Givord, 1977). IC-OkMV and N-OkMV can be distinguished on the basis of cross-serological reaction with some other tymoviruses and differential host susceptibility (Bozarth et al., 1977).

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ASTERIDAE.

General Symptoms in Plants Virus affects the photosynthetic system. Symptoms are expressed in the leaf include chlorosis and development of patterns or markings that are visible in the intercostal regions. Intercostal regions show chlorosis. Leaves with mosaic. Symptoms mosaic, vein chlorosis and banding in Malvaceae. Other families show interveinal chlorosis, vein chlorosis and vein banding. In all families plants are often stunted.

Severity and Occurrence of Disease

Host: Signs and symptoms persist (in all other families except Malvaceae).

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seeds.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Coleoptera; Podagrica decolorata in Cöte d'Ivoire (Givord and den Boer, 1980, Podagrica uniforma, P. sjostedti in Nigeria (Lana and Taylor, 1976; Atiri, 1984). Possibly Bemisia tabaci is a vector in Nigeria (Lana and Taylor, 1976), but needs confirmation. Virus is not transmitted by Aphis gossypii, Chrysolagria cuprina, Lagria villosa, Medythia quaterna, Ootheca mutabilis, Nisotra dilecta. Virus is transmitted in a non-persistent manner.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in many families. Susceptible host species are found in the Family Amaranthaceae, Apocynaceae, Bombacaceae, Caryophyllaceae, Chenopodiaceae, Cruciferae, Cucurbitaceae, Leguminosae-Papilionoideae, Malvaceae (12 /12), Passifloraceae, Pedaliaceae, Polemoniaceae, Rubiaceae, Solanaceae, Sterculiaceae, Tetragoniaceae, Tiliaceae, Tropaeolaceae, Umbelliferae. The following species were susceptible to experimental virus infection: Abelmoschus esculentus, Abutilon hirtum, Adansonia digitata, Amaranthus retroflexus, Apium graveolens, Arachis hypogaea, Borreria intricans, Cajanus cajan, Capsella bursa-pastoris, Catharanthus roseus, Ceiba pentandra, Chenopodium album, Chenopodium amaranticolor, Chenopodium ambrosioides, Chenopodium foetidum, Chenopodium hybridum, Chenopodium quinoa, Citrullus lanatus, Corchorus (no species name provided), Corchorus olitorius, Crotalaria juncea, Cucumis sativus, Cucurbita pepo, Daucus carota, Dianthus barbatus, Gomphrena globosa, Gossypium hirsutum, Hibiscus cannabinus, Hibiscus rosa-sinensis, Hibiscus sabdariffa, Lathyrus odoratus, Lavatera trimestris, Malvastrum coromandelianum, Matthiola incana, Momordica balsamina, Nicotiana benthamiana, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana glutinosa x N. clevelandii, Nicotiana megalosiphon, Nicotiana rustica, Nicotiana x edwardsonii, Passiflora edulis, Phaseolus lunatus, Phaseolus vulgaris, Phlox drummondii, Physalis angulata, Pisum sativum, Sesamum indicum, Sida acuta, Sida linifolia, Sida rhomboidea, Sinapis alba, Solanum melongena, Solanum nigrum, Spinacia oleracea, Tetragonia tetragonioides, Theobroma cacao, Trifolium incarnatum, Trifolium pratense, Tropaeolum majus, Urena lobata, Vicia faba, Vigna radiata, Vigna subterranea, Vigna unguiculata.

Host:
Experimentally infected hosts mainly show symptoms of regular vein chlorosis, large chlorotic bands along principal veins, spotting or dotting, chlorotic mosaic, chlorotic local lesions, stunting.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Apocynaceae, or Chenopodiaceae, Compositae, Cruciferae, Cucurbitaceae, or Euphorbiaceae, Gramineae, Leguminosae-Papilionoideae, Malvaceae (1 /12), or Scrophulariaceae, Solanaceae, Umbelliferae. Species inoculated with virus that do not show signs of susceptibility: Amaranthus caudatus, Antirrhinum majus, Beta vulgaris, Brassica campestris ssp. pekinensis, Brassica oleracea var. botrytis, Calendula officinalis, Capsicum annuum, Capsicum frutescens, Catharanthus roseus, Celosia argentea, Cheiranthus cheiri, Citrullus lanatus, Cucumis melo, Cucurbita maxima, Cucurbita pepo, Datura metel, Datura stramonium, Glycine max, Gomphrena globosa, Gossypium hirsutum, Hordeum vulgare, Hyoscyamus niger, Lactuca sativa, Lycopersicon esculentum, Lycopersicon pimpinellifolium, Macroptilium lathyroides, Medicago sativa, Melilotus albus, Nicandra physalodes, Nicotiana glutinosa, Nicotiana rustica, Nicotiana tabacum, Pastinaca sativa, Petunia x hybrida, Phaseolus vulgaris, Physalis floridana, Physalis peruviana, Pisum sativum, Raphanus sativus, Ricinus communis, Senecio vulgaris, Setaria italica, Solanum tuberosum, Sonchus oleraceus, Trifolium repens, Triticum aestivum, Vicia faba, Zea mays, Zinnia elegans.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium amaranticolor — systemic chlorotic spotting and line patterns.

Cucumis sativus — large chlorotic local lesions, then systemic vein chlorosis.

Arachis hypogaea — dark and light green mosaic.

Vigna unguiculata — fine systemic vein chlorosis and mosaic.

Nicotiana clevelandii — systemic dark green and whitish mosaic, dwarfing. Diagnostic host: insusceptible host species Zinnia elegans, Brassica campestris ssp. pekinensis, Cucurbita pepo, Phaseolus vulgaris, Datura stramonium.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Cucumis sativus in temperate regions. Abelmoschus esculentus in tropical regions.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
no reliable local lesion host known. Abelmoschus esculentus (W).

References to host data: Givord (1977; 1979, Givord and Hirth (1973, Igwegbe (1983, Lana and Bozarth (1975).

Histopathology: Virus can be best detected in leaves and roots. Virions are found in the nucleus (containing masses of empty protein shells), or mitochondria (containing vacuole-like vesicles with empty protein shell-like structures).

Cytopathology: Other cellular changes include the small double membrane vesicles, that form in the peripheries of chloroplasts, which become rounded, clumped, vacuolated and disorganised (Lesemann, 1977).

Geographical Distribution

The virus occurs in Cote d'Ivoire and Nigeria.

Ecology, Epidemiology and Control

Studies reported by natural and synthetic chemicals are known for control (Atiri, 1991). Tolerant cultivars; Perkins Long Pod in Cöte d'Ivoire (Givord, 1980), NHAE 621 and NHAE 117 in Nigeria (Atiri, 1983).

List of Strains and Isolates in the Species

VMG-CI (IC. OkMV) from Abelmoschus esculentus, Cöte d'Ivoire; VM-HR, from H. rosa-sinensis, Cöte d'Ivoire; VMG-Nig (N.OkMV) from A. esculentus in Nigeria (Givord, 1977; Bozarth et al., 1977).

References

Atiri, G.I. (1983). Ann. appl. Biol. 102 no Suppl.: 132.

Atiri, G.I. (1984). Ann. appl. Biol. 104: 261.

Atiri, G.I., Ivbijaro, M.F. and Oladele, AD. (1991). Trop. Agric., Trin. 68: 178.

Bozarth, R.F., Lana, AO, Koenig, R. and Reese, J. (1977). Phytopathology 67: 735.

Genevaux, M., Pinck, M. and Duranton, H.M. (1976). Ann. Microbiol. 127: 47.

Givord, L. (1977). Annls. Phytopath. 9: 53.

Givord, L. (1978). Pl. Dis. Reptr 62: 412.

Givord, L. (1979). Agron. trop. 34: 88.

Givord, L. (1980). Ph.D. Thesis, University Louis Pasteur of Strasbourg, France.

Givord, L. and Den Boer, L. (1980). Ann. appl. Biol. 94: 235.

Givord, L. and Hirth, L. (1973). Ann. appl. Biol. 74: 359.

Givord, L. and Koenig, R. (1974). CMI/AAB Descr. Pl. Viruses No. 128, 4 pp.

Givord, L., Pfeiffer, P. and Hirth, L. (1972). C. r. Acad. Sci. Paris 275: 1563.

Hirth, L. and Givord, L. (1988). In: The Plant Viruses. Vol. 3, Polyhedral virions with monopartite RNA genomes, p. 163; ed. R. Koenig, Plenum Press, New York.

Igwegbe, E.C.K. (1983). Plant Dis. 67: 320.

Koenig, R. (1976). Virology 72: 1.

Koenig, R. and Givord, L. (1974). Virology 58: 119.

Lana, AO and Ajibola Taylor, T. (1976). Ann. appl. Biol. 82: 361.

Lana, AO and Bozarth, R.F. (1975). Phytopath. Z. 83: 77.

Lesemann, D.-E. (1977). Phytopath. Z. 90: 315.

Marshall, B. and Matthews, R.E.F. (1981). Virology 110: 253.

Paul, H.L., Gibbs, A and Wittmann-Liebold, B. (1980). Intervirology 13: 99.

The following generic references are cited in the most recent ICTV REport .

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 533 by L. Givord, 1984. Revised 1991.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 128.



Limit search to: Title & Body Title Document Path
Show Reverse Sort

DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

ICTVdB and DELTA related References

Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
Copyright © 2002    International Committee on Taxonomy of Viruses.    All rights reserved.


Additional access points to virus species lists, descriptions and images on the web:

Species catalogue                     iSpecies.org - a species search engine           a species search engine

Google Analytics      Google Analytics: activity view