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00.077.0.01.010. Erysimum latent virus

Cite this publication as: ICTVdB Management (2006). 00.077.0.01.010. Erysimum latent virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Germany.

Host of Isolate and Habitat Details
Source of isolate: Erysimum helveticum, E. perovskianum, E. pulchellum, E. sylvestre, E. crepidifolium, Fibigia clypata, Arabis ludoviciana, Barbarea vulgaris.

Natural host and symptoms
Erysimum ssp. — latent, mosaics.

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.077.0.01.010. Virus accession number: 77001010. Obsolete virus code: 77.0.1.0.008; superceded accession number: 77010008.
NCBI Taxon Identifier NCBI Taxonomy ID: 12152.

Name, Synonyms and Lineage

ICTV approved acronym: ErLV. Virus is an ICTV approved species of the genus 00.077.0.01. Tymovirus; family 00.077. Tymoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 27 nm. Capsids appear round. The capsomer arrangement is clearly visible.

Electron microscopic preparation and references: Virus preparation contains many virions. Reference for electron microscopic methods: Shukla and Gough (1980).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.26 g cm-3, or 1.42 g cm-3. There are 2 sedimenting component(s) found in purified preparations. The sedimentation coefficient is of the fastest 113 S20w; of the other(s) are 59 S20w. Isoelectric point pH is 5.4. A260/A280 ratio is 1.76 (B). The thermal inactivation point (TIP) is at 76-78°C. The longevity in vitro (LIV) is 21 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 6. The infectivity is not changed by treatment with ether; retained when deproteinized with proteases; retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 32% of the virion by weight. The genome is monopartite. Only one particle size of linear, single-stranded RNA is recovered. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA that is mRNA. The complete genome is 6034 nucleotides long, is fully sequenced, complete sequence is 6034 nucleotides long. The genome has a base ratio of 15.6 % guanine; 23.9 % adenine; 34.5 % cytosine; 26 % uracil. The 5'-end of the genome has a methylated nucleotide cap.

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 68% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Reference to method of preparation: Srifah et al. (1990).

Reference to amino acid sequence or composition Srifah et al. (1990).

Non-Structural Proteins: Virus-coded non-structural proteins have been identified by sequence analysis and 2 non-structural protein(s) are found.

Lipids

Lipids are not reported.

Genome Organization and Replication

By itself, genomic nucleic acid is infectious.

Transcription: Sub-genomic RNA is present in infected cells; a transcription of virion proteins.

Translation: Coat protein mRNA is translated in the cytoplasm.

The genome replicates in in association with chloroplasts (in vesicles in the outer membrane).

Antigenicity

The virus is serologically related to Andean potato latent, ononis yellow mosaic and okra mosaic viruses.

Genome sequence homologous with all other tymoviruses but is the most distinct of the group.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

General Symptoms in Plants Symptoms mosaics.

Severity and Occurrence of Disease

Host: Infection is not apparent. Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; not transmitted by contact between hosts; not transmitted by seeds; not transmitted by pollen.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Coleoptera; Phyllotreta ssp. Virus is transmitted in a semi-persistent manner.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in many families. Susceptible host species are found in the Family Amaranthaceae, Apocynaceae, Caryophyllaceae, Cruciferae, Cucurbitaceae, Labiatae, Leguminosae-Papilionoideae, Resedaceae, Solanaceae, Violaceae. The following species were susceptible to experimental virus infection: Arabidopsis thaliana, Arabis ludoviciana, Barbarea vulgaris, Brassica campestris ssp. chinensis, Brassica campestris ssp. napus, Brassica campestris ssp. pekinensis, Brassica campestris ssp. rapa, Brassica juncea, Brassica oleracea var. botrytis, Camelina sativa, Capsella bursa-pastoris, Catharanthus roseus, Celosia argentea, Cucumis sativus, Erysimum crepidifolium, Erysimum helveticum, Erysimum perovskianum, Erysimum pulchellum, Erysimum sylvestre, Fibigia clypata, Lunaria annua, Nicotiana glutinosa, Nicotiana megalosiphon, Ocimum basilicum, Reseda odorata, Sinapis alba, Solanum integrifolium, Stellaria media, Trigonella caerulea, Viola cornuta.

Host:
Experimentally infected hosts mainly show symptoms of local lesions, vein clearing, mosaics.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Bignoniaceae, Boraginaceae, Campanulaceae, Caryophyllaceae, Chenopodiaceae, Compositae, Convolvulaceae, or Cruciferae, Euphorbiaceae, Gesneriaceae, Labiatae, or Leguminosae-Papilionoideae, Malvaceae, Myrtaceae, Papaveraceae, or Plantaginaceae, Polemoniaceae, Polygonaceae, Ranunculaceae, or Rosaceae, Scrophulariaceae, Solanaceae, Tropaeolaceae, Umbelliferae. Species inoculated with virus that do not show signs of susceptibility: Anthriscus cerefolium, Antirrhinum majus, Apium graveolens, Arachis hypogaea, Bellis perennis, Beta vulgaris, Brassica oleracea var. capitata, Catalpa bignonioides, Cheiranthus cheiri, Chenopodium amaranticolor, Chenopodium foetidum, Chenopodium murale, Chenopodium quinoa, Delphinium hybridum, Eucalyptus cloeziana, Euphorbia marginata, Fagopyrum esculentum, Fragaria vesca, Gossypium hirsutum, Gypsophila elegans, Helianthus annuus, Ipomoea nil, Lactuca sativa, Lobelia erinus, Matthiola incana, Myosotis sylvatica, Nicotiana clevelandii, Nicotiana tabacum, Papaver nudicaule, Pastinaca sativa, Petroselinum crispum, Petunia x hybrida, Phaseolus vulgaris, Phlox drummondii, Pisum sativum, Plantago major, Podolepis robusta, Raphanus sativus, Salvia splendens, Sinningia speciosa, Spinacia oleracea, Tropaeolum majus, Vicia faba, Vigna unguiculata.

Diagnostic Hosts

Diagnostic host species and symptoms:

Brassica campestris ssp. napus, B. campestris ssp. pekinensis — local lesions and mosaic.

Cucumis sativus, Nicotiana glutinosa — mottle. Capsella bursa-pastoris — mosaic.

Ocimum basilicum, Sinapis alba — local lesions, mosaic.

Stellaria media, Lunaria annua — vein clearing and mottling.

Erysimum ssp. — latent. Diagnostic host: insusceptible host species Chenopodium quinoa, Nicotiana clevelandii, Nicotiana tabacum, Phaseolus vulgaris, Pisum sativum.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Brassica campestris ssp. pekinensis, B. juncea, B. campestris ssp. napus, Lunaria annua.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Brassica campestris ssp. napus (L).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm.

Cytopathology: Inclusions are not present in infected cells.

Geographical Distribution

The virus occurs in Germany.

References

Gough, K., Lilley, G.G., Shukla, D.D. and Woods, E.F. (1982). Aust. J. biol. Sci. 35: 5.

Shukla, D.D. and Gough, K. (1980). CMI/AAB Descr. Pl. Viruses No. 222, 4 pp.

Shukla, D.D., Koenig, R., Gough, K.H., Huth, H. and Lesemann, D.-E. (1980). Phytopathology 70: 382.

Shukla, D.D., Proll, E., Schmelzer, K. and Schmidt, H.B. (1973). Acta phytopath. Acad. Sci. Hung. 8: 91.

Srifah, P., Keese, P., Shukla, D.D. and Gibbs, AJ. (1990). J. gen. Virol. 71: 3085. The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 327 by A.J. Gibbs, 1991. A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 222.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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