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00.074.0.03. Necrovirus

Cite this publication as: ICTVdB Management (2006). 00.074.0.03. Necrovirus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Classification

This is a description of a plant virus at the genus level.

ICTVdB Virus Code: 00.074.0.03. Virus accession number: 074003GE. Obsolete virus code: 44.0.1.; superceded accession number: 44010000.
NCBI Taxon Identifier NCBI Taxonomy ID: 12052.

Name, Synonyms and Lineage

Synonym(s): Tobacco necrosis virus group. Virus is of the family 00.074. Tombusviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 26-28.66-30 nm. Capsids appear round, or hexagonal in outline. The capsomer arrangement is not obvious. The capsid consists of 32 capsomers. Virus preparations contain more than one particle component (i.e. satellite virus TNV of 17 nm is associated with virus and depends on TNV for replication).

Electron microscopic preparation and references: Virus preparation contains few virions, or many virions.

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.333-1.367-1.399 g cm-3. There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 99-118.6-136 S20w. Isoelectric point pH is 4.5. A260/A280 ratio is 1.61. The thermal inactivation point (TIP) is at 80-87.5-95°C. The longevity in vitro (LIV) is 7-46.83-63 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 4, or 7, or 9-10. The infectivity is retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 18-18.5-19% of the virion by weight. The genome is monopartite. Only one particle size of linear, positive-sense, single-stranded RNA is recovered. Minor species of non-genomic nucleic acid are not found in virions. The complete genome is 3660-3760 nucleotides long. Genome is sequenced, but only an estimate is available, complete sequence is 3660-3760 nucleotides long. The genome has a base ratio of 24 % guanine; 27.5 % adenine; 22.5 % cytosine; 25.5 % uracil. The cap sequence type is ppApGpUp...

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 72-76.5-81% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s).

Lipids

Lipids are not reported.

Transcription: Sub-genomic RNA is present in infected cells.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass CARYOPHYLLIDAE, or ROSIDAE, or ASTERIDAE.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; not transmitted by seeds; not transmitted by pollen.

Vector Transmission:
Virus is transmitted by fungi; of the order Chytridiales.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in many families, several families. Susceptible host species are found in the Family Amaranthaceae, Apocynaceae, Caryophyllaceae, Chenopodiaceae, Compositae, Cruciferae, Cucurbitaceae, Euphorbiaceae, Gentianaceae, Gesneriaceae, Gramineae, Leguminosae-Papilionoideae, Liliaceae, Onagraceae, Pedaliaceae, Rosaceae, Scrophulariaceae, Solanaceae, Tetragoniaceae. The following species were susceptible to experimental virus infection: Amaranthus caudatus, Antirrhinum majus, Arachis hypogaea, Avena sativa, Beta vulgaris, Brassica campestris ssp. rapa, Brassica oleracea var. capitata, Capsicum frutescens, Catharanthus roseus, Chenopodium album, Chenopodium amaranticolor, Chenopodium ambrosioides, Chenopodium quinoa, Cichorium endiva, Clarkia amoena, Cucumis melo, Cucumis sativus, Cucurbita maxima, Cucurbita moschata, Cucurbita pepo, Cyamopsis tetragonoloba, Datura stramonium, Dianthus barbatus (1), Dianthus caryophyllus, Eustoma russellianum, Fragaria vesca, Glycine max, Gomphrena globosa, Gypsophila elegans, Helianthus annuus, Hordeum vulgare, Hyoscyamus niger, Lactuca sativa, Lathyrus odoratus, Lupinus albus, Lycopersicon esculentum, Nicandra physalodes, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana rustica, Nicotiana sylvestris, Nicotiana tabacum, Petunia x hybrida, Phaseolus vulgaris, Pisum sativum, Ricinus communis, Saponaria vaccaria, Sesamum indicum, Sinningia speciosa, Solanum nodiflorum, Sonchus oleraceus, Spinacia oleracea, Tetragonia tetragonioides, Trifolium incarnatum, Trifolium pratense, Trifolium repens, Triticum aestivum, Tulipa gesneriana, Vicia faba, Vigna radiata, Vigna unguiculata, Vigna unguiculata ssp. sesquipedalis , Zea mays, Zinnia elegans.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Boraginaceae, Caryophyllaceae, Compositae, Convolvulaceae, Cruciferae, Cucurbitaceae, Euphorbiaceae, or Gramineae, Leguminosae-Papilionoideae, Solanaceae, Tropaeolaceae, or Umbelliferae. Species inoculated with virus that do not show signs of susceptibility: Avena sativa, Brassica campestris ssp. rapa, Calendula officinalis, Capsicum annuum, Cucumis sativus, Cucurbita pepo, Daucus carota, Dianthus barbatus, Dianthus caryophyllus, Euphorbia marginata, Hordeum vulgare, Ipomoea setosa, Lycopersicon esculentum, Medicago sativa, Myosotis sylvatica, Nicotiana benthamiana, Nicotiana debneyi, Nicotiana rustica, Physalis peruviana, Raphanus sativus, Secale cereale, Solanum melongena, Triticum durum, Tropaeolum majus, Vicia faba, Vigna radiata, Zea mays.

Histopathology: Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are crystals, or membranous bodies. Inclusions contain mature virions, or do not contain mature virions.

Geographical Distribution

The virus is probably distributed worldwide. The virus occurs in Italy, or Japan.

Taxonomic Structure of the Genus

Type species 00.074.0.03.001. Tobacco necrosis A virus .

Species in the Genus

List of Species in the Genus.
Tentative Species in the Genus.

Data Sources and Contributions

The description has been compiled from data in the ICTV Report presented by Lommel SA.

References

The following generic references are cited in the most recent ICTV Report.

PubMed References.

Images

Taxon images: • EM from IACR Rothamsted.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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