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00.071.0.01.020. Zucchini green mottle mosaic virus

Cite this publication as: ICTVdB Management (2006). 00.071.0.01.020. Zucchini green mottle mosaic virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Chonju; Chonbook Province; Korea (South).

Host of Isolate and Habitat Details
Source of isolate: zucchini squash Cucurbita pepo.
Virus was isolated from seeds (imported from China (virus is seed-transmitted)).

Reference to Isolation Report
Ryu,K.H., Min,B.E., Choi,G.S., Choi,S.H., Kwon,S.B., Noh,G.M., Yoon,J.Y., Choi,Y.M., Jang,S.H., Lee,G.P., Cho,K.H. and Park,W.M. (2000) Zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat protein gene with that of kyuri green mottle mosaic virus. Arch. Virol. 145, 2325-2333; PubMed ID 21073712.

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.071.0.01.020. Virus accession number: 71001020. Obsolete virus code: 71.0.1.0.020; superceded accession number: 71010020.
NCBI Taxon Identifier NCBI Taxonomy ID: 111418.

Name, Synonyms and Lineage

The taxon is a novel virus not yet listed in the current ICTV Report (submitted by Ki Hyun Ryu, 16-Jan-2000).

Virus is a novel species of the genus 00.071.0.01. Tobamovirus; not assigned to a family; not assigned to an order.

Virion Properties

Distinct viral structures are visible in thin sections of infected tissue.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. The capsid is rod-shaped, straight with a clear modal length with a length of 300 nm and a width of 18 nm. Axial canal is distinct. Basic helix is obvious.

Virion populations are comprised of particles of uniform size.

Electron microscopic preparation and references: Virus preparation contains many virions.

Physicochemical and Physical Properties

A260/A280 ratio is 1.3. The thermal inactivation point (TIP) is at 90°C. The longevity in vitro (LIV) is over 120 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 6. Under in vitro conditions virions are stable when stored at -20°C to 20°C. The infectivity is retained when deproteinized with proteases; retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 5% of the virion by weight. The genome is not segmented and contains a single molecule of linear positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA, that is mRNA (coding for the coat protein). The complete genome is 6500 nucleotides long. The RNA is sequenced, complete sequence is about 6500 nucleotides long. The subgenomic RNA-2 is 700 bp long has the accession number
[AJ252188], CP gene for coat protein. The genome has no intergenic poly (A) region. The sequence has no triple gene block coding arrangement. Each virion contains a full length copy and shorter copies (of subgenomic mRNA).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 95% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins located in the capsid.

Structural Proteins: Capsid protein CP has a molecular mass of 17181-17183 Da (161 aa, sequence has the accession number [Q9IW11]; is involved in viral encapsidation.

Lipids

Lipids are not reported.

Carbohydrates

Carbohydrates have not been reported.

Assembly and Egress: Viruses assemble in the cytoplasm.

Maturation: The mature virus is found in the host cell cytoplasm. In thin sections the mature virus is seen in crystalline arrays.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass DILLENIIDAE; Order Violales Family Cucurbitaceae;
Family Cucurbitaceae. Virus found in Cucurbita pepo.

General Symptoms in Plants Symptoms are expressed in the leaf and infructescence; include stunting, deformation, and reduced vigor; include stunting of all plant parts; include deformation of leaves and fruits. Symptoms in leaves include size reduction and development of patterns or markings. Leaf margin can be chlorotic and changed in color. Leaves with mosaic and mottle have green mosaic and green mottle. Fruit symptoms include malformation, size reduction, and change in texture.

Transmission and Vector Relationships

Virus is transmitted by seeds.

Non-Vector Transmission: The likelihood of viral transmission by direct contact is significant.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Cruciferae, Cucurbitaceae, Solanaceae. The following species were susceptible to experimental virus infection: Chenopodium amaranticolor, Citrullus lanatus, Cucumis sativus, Cucurbita moschata, Cucurbita pepo, Datura stramonium, Lagenaria siceraria, and Nicotiana benthamiana.

Diagnostic Hosts

Diagnostic host species and symptoms:
Datura stramonium — necrotic local lesion. Infection is local. Symptoms visible; after 3 days. Local symptoms include necrotic spots. Local lesions found on leaf. Lesions are necrotic. Diagnostically insusceptible host species: Nicotiana tabacum.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are zucchini squash. Maintenance and propagation hosts are Cucurbita pepo.

Geographical Distribution

Geographical distribution of the virus is probably restricted. The virus occurs in Korea (South).

List of Strains and Isolates in the Species

ZU (type isolate) and ZT1.

References

The following generic references are cited in the most recent ICTV Report.

PubMed References.

Contributor

Data have been submitted online to ICTVdB on 16-01-2000 by
Ryu Ki Hyun;
Plant Virus GenBank, Department of Life and Environmental Design;
Seoul Women's University;
Nowon-ku;
Seoul

; 139-774;
Korea;
Tel: + +82-2-970-5618;
Fax: + +82-2-970-5610;
email: [mailto] ryu@swu.ac.kr.

Taxonomic Proposals and Changes

A taxonomic proposal has been submitted to the ICTV by the Plant Virus Subcommittee, Study Group for Tobamovirus, to create a new taxon. This novel virus has been isolated from zucchini squash plants grown from seeds imported from China.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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