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00.071.0.01.004. Kyuri green mottle mosaic virus

Cite this publication as: ICTVdB Management (2006). 00.071.0.01.004. Kyuri green mottle mosaic virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Japan.

Host of Isolate and Habitat Details
Source of isolate: Cucumis sativus.

Natural host and symptoms
Cucumis sativus — mosaic, severe fruit mottling and malformation.

Reference to Isolation Report
Inouye et al. (1967a).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.071.0.01.004. Virus accession number: 71001004. Obsolete virus code: 71.0.1.0.004; superceded accession number: 71010004.
NCBI Taxon Identifier NCBI Taxonomy ID: 111970.

Name, Synonyms and Lineage

Synonym(s): Japanese CV3, Cucumber green mottle mosaic virus - cucumber strain or strain C (Francki et al., 1986). ICTV approved acronym: KGMMV. Virus is an ICTV approved species of the genus 00.071.0.01. Tobamovirus; not assigned to a family.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. The capsid is rod-shaped, straight with a clear modal length with a length of c. 300 nm and a width of c. 15 nm. Axial canal is distinct. Basic helix is obvious.

Electron microscopic preparation and references: Virus preparation contains many virions. No special treatments needed. Reference for electron microscopic methods: Tung and Knight (1972, Francki and McLean (1968).

Physicochemical and Physical Properties

The infectivity is retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes c. 5% of the virion by weight. The genome is not segmented and contains a single molecule of linear positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA, that is mRNA. The complete genome is 6500 nucleotides long. Genome is sequenced, but only an estimate is available, complete sequence is 6500 nucleotides long. Reference to nucleotide sequence Peden and Symons (1973).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about c. 95% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein CP has a molecular mass of 17182 Da (161 aa, sequence has the accession number [Q9PWU0]; is the coat protein.

Reference to method of preparation: Fraenkel-Conrat (1957, Tung and Knight (1972).

Reference to amino acid sequence or composition Nozu et al. (1971, Tung and Knight (1972, Kurachi et al. (1972).

Lipids

Lipids are not reported.

Genome Organization and Replication

By itself, genomic nucleic acid is infectious.

Transcription: Sub-genomic RNA is present in infected cells; encoding the coat protein.

Antigenicity

The virus is serologically related to Cucumber green mottle mosaic (SDI:6), ribgrass mosaic (SDI:5), Frangipani mosaic (SDI:5), Odontoglossum ringspot (SDI:4), tobacco mosaic (SDI:7) and tobacco mild green mosaic (SDI:6) viruses. The virus does not show serological relationships to Sunn-hemp mosaic.

Biologically kyuri green mottle mosaic and cucumber green mottle mosaic viruses are similar but have little genomic sequence homology and are serologically distantly related.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms).

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is not transmitted by a vector. Virus is transmitted by mechanical inoculation.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Cucurbitaceae, Solanaceae. The following species were susceptible to experimental virus infection: Cucumis melo, Cucumis sativus, Cucurbita maxima, Cucurbita moschata, Cucurbita pepo, Datura stramonium, Nicotiana tabacum, Petunia x hybrida.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Apocynaceae, or Chenopodiaceae, Compositae, Cruciferae, Leguminosae-Papilionoideae, or Solanaceae, Tetragoniaceae. Species inoculated with virus that do not show signs of susceptibility: Beta vulgaris, Catharanthus roseus, Chenopodium amaranticolor, Glycine max, Gomphrena globosa, Lactuca sativa, Lycopersicon esculentum, Nicotiana glutinosa, Nicotiana rustica, Phaseolus vulgaris, Raphanus sativus, Spinacia oleracea, Tetragonia tetragonioides, Vicia faba, Zinnia elegans.

Diagnostic Hosts

Diagnostic host species and symptoms:

Cucumis sativus — mottling, and fruit malformation.

Datura stramonium, Petunia x hybrida — diffuse chlorotic local lesions; not systemic.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Cucumis sativus.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Datura stramonium (L), Cucumis sativus (W).

References to host data: Inouye et al. (1967b).

Histopathology: Virus can be best detected in leaves, mesophyll, epidermis, vascular parenchyma, xylem, phloem, companion cells and all parts of the host plant. Virions are found in the cytoplasm and cell vacuole.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are crystals. Inclusions contain mature virions. Other cellular changes include vesiculation of mitochondria.

Geographical Distribution

The virus occurs in Japan.

List of Strains and Isolates in the Species

C and Y isolates or strains.

Comments

Although long considered to be a strain of Cucumber green mottle mosaic virus, this virus has been shown to be a distinct tobamovirus (Francki et al., 1986).

References

Fraenkel-Conrat, H. (1957). Virology 4: 1.

Francki, RIB. and McLean, GD (1968). Aust. J. biol. Sci. 21: 1311.

Francki, RIB., Hu, J. and Palukaitis, P. (1986). Intervirology 26: 156.

Inouye, T., Inouye, N., Asatani, M. and Mitsuhata, K. (1967a). Nogaku Kenkyu 51: 175.

Inouye, T., Inouye, N., Asatani, M. and Mitsuhata, K. (1967b). Ber. Ohara Inst. landw. Biol. 14: 49.

Kurachi, K., Funatsu, G., Funatsu, M. and Hidaka, S. (1972). Agric. Biol. Chem. 36: 1109.

Nozu, Y., Tochihara, H., Komuro, Y. and Okada, Y. (1971). Virology 45: 577.

Okada, Y. (1986). In: The Plant Viruses. Vol. 2, The Rod-Shaped Plant Viruses, p. 267; eds M.H.V. van Regenmortel and H. Fraenkel-Conrat Plenum Press, New York.

Peden, K.W.C. and Symons, RH (1973). Virology 53: 487.

Tung, J.S. and Knight, CA (1972). Virology 48: 574.

The following generic references are cited in the most recent ICTV Report.

PubMed References. A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 432 by R.I.B. Francki, 1988.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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