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00.069.0.01.004. Rice hoja blanca virus

Cite this publication as: ICTVdB Management (2006). 00.069.0.01.004. Rice hoja blanca virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Colombia.

Host of Isolate and Habitat Details
Source of isolate: Oryza sativa.

Natural host and symptoms
Oryza sativa, wild Oryza ssp. — systemic necrosis, systemic chlorotic striping in early infections.

Reference to Isolation Report
Garc‚s (1940, Morales and Niessen (1983).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.069.0.01.004. Virus accession number: 69001004. Obsolete virus code: 69.0.1.0.004; superceded accession number: 69010004.
NCBI Taxon Identifier NCBI Taxonomy ID: 12332.

Name, Synonyms and Lineage

Synonym(s): Echinochloa hoja blanca virus (Falk et al., 1987). ICTV approved acronym: RHBV. Virus is an ICTV approved species of the genus 00.069.0.01. Tenuivirus; not assigned to a family.

Virion Properties

Morphology

Virions consist of a nucleocapsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. Virus preparations contain more than one particle component. The nucleocapsid is filamentous; and segments have a length proportional to the size of their RNA and a width of 3 nm. The nucleocapsid is segmented.

Electron microscopic preparation and references: Virus preparation contains few virions. Reference for electron microscopic methods: Morales and Niessen (1983).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.288 g cm-3. There are 1-2 sedimenting component(s) found in purified preparations (in caesium salts), or 1-3 sedimenting component(s) found in purified preparations (in sucrose). Isoelectric point pH is 4.5.

Nucleic Acid

The Mr of the genome constitutes 12% of the virion by weight (determined from A260/280). The genome is segmented and consists of four segments of linear, negative-sense and ambisense, single-stranded RNA. The complete genome is 17590 nucleotides long, is fully or partially sequenced, complete sequence is 9800 nucleotides long. Sequence has the accession number

[L07940] Em(40)_vi:HBVMNS3NP Gb(84)_vi:HBVMNS3NP Rice hoja blanca virus non-structural protein 3 (NS-3) and nucleoprotein (N-protein) genes, c
[L14952] Em(40)_vi:HBVNS4VC Gb(84)_vi:HBVNS4VC Rice hoja blanca virus nonstructural protein (NS4) and (NS4vc) genes, complete cds's. 3/94 1.
[L39989] Em(43)_vi:Hbvpv2a Gb(89)_vi:Hbvpv2a Rice hoja blanca virus membrane associated protein (pv2) gene, complete cds. 3/95 988bp. Sequence is sequenced, complete sequence is about 3500 nucleotides long, is sequenced, complete sequence is 2300 nucleotides long, has been sequenced, but only an estimate is provided; complete sequence is 1990 nucleotides long.

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s).

Non-Structural Proteins: One virus-coded non-structural proteins have been isolated (Gingery et al. (1981).

Lipids

Lipids are not reported.

Antigenicity

The virus is serologically related to echinochloa hoja blanca virus. The virus does not show serological relationships to maize stripe and rice stripe viruses.

Diagnostics and Reference Collections

The best tests for diagnosis are rice hoja blanca is the only virus known to affect rice in the Americas.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is not transmitted by mechanical inoculation; not transmitted by contact between hosts; not transmitted by seeds.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Hemiptera, family Delphacidae; Sogatodes orizicola. Virus is transmitted in a persistent manner; retained when the vector moults; replicates in the vector; transmitted congenitally to the progeny of the vector.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Gramineae. The following species were susceptible to experimental virus infection: Avena sativa, Digitaria, Hordeum vulgare, Leptochloa, Oryza sativa, Secale cereale, Triticum aestivum.

Diagnostic Hosts

Diagnostic host species and symptoms:

Avena sativa, Digitaria ssp., Hordeum vulgare, Leptochloa ssp., Oryza sativa cv. Bluebonnet-50, Secale cereale, Triticum aestivum — chlorotic stripes. Diagnostic host: insusceptible host species host range restricted to Gramineae.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are purified from susceptible Oryza sativa cultivars only.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
susceptible Oryza sativa cultivars (W).

References to host data: Lamey et al. (1964).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are amorphous X-bodies. Inclusions contain mature virions. Other cellular changes include disorganisation of chloroplasts, mitochondria and nuclei.

Geographical Distribution

The virus spreads in South and Central Americas. The virus occurs in Colombia, Costa Rica, Cuba, the Dominican Republic, Ecuador, El Salvador, Honduras, Nicaragua, Panama, Peru, and Venezuela (and elsewhere in the Caribbean). The virus is found, but with no evidence of proliferation, in the U.S.A.

Ecology, Epidemiology and Control

Studies reported by Everett and Lamey (1969, Lamey (1967).

List of Strains and Isolates in the Species

Echinochloa strain (Morales and Niessen, 1985).

References

Everett, T.R. and Lamey, H.A. (1969). In: Viruses, Vectors, and Vegetation; ed. K. Maramorosch. Interscience, New York.

Flak, B.W., Morales, F.J., Tsai, J.H. and Niessen, A (1987). Phytopathology 77: 196.

Garc‚s (1940). In: Projects in Plant Pathology at the Palmira Experimental Station, unnumbered report to the Ministry of Economy, Bogota.

Gingery, R.E., Nault, L.R. and Bradfute, O.E. (1981). Virology 112: 99.

Lamey, H.A. (1967). In: The Virus Diseases of the Rice Plant, 354 pp. John Hopkins, Baltimore.

Lamey, H.A., McMillan, W.W. and McGuire, J.U. (1964). Phytopathology 54: 536.

Morales, F.J. and Niessen, AI. (1983). Phytopathology 73: 971.

Morales, F.J. and Niessen, AI. (1985). AAB Descr. Pl. Viruses No. 299, 4 pp.

Ramirez, B.C., Macaya, G., Calvert, L.A. and Haenni, A-L. (1992). J. gen. Virol. 73: 1457.

Ramirez, B.C., Lozano, I., Constantino, L.-M., Haenni, A-L. and Calvert, L.A. (1993). J. gen. Virol. 74: 2463. The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 689 by F.J. Morales, 1984. Revised 1989.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 299.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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