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00.067.0.01.004. Lucerne transient streak virus

Cite this publication as: ICTVdB Management (2006). 00.067.0.01.004. Lucerne transient streak virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Victoria; Australia.

Host of Isolate and Habitat Details
Source of isolate: Medicago sativa.

Natural host and symptoms
Medicago sativa — vein yellowing.

Reference to Isolation Report
Blackstock (1974; 1978).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.067.0.01.004. Virus accession number: 67001004. Obsolete virus code: 67.0.1.0.004; superceded accession number: 67010004.
NCBI Taxon Identifier NCBI Taxonomy ID: 12470.

Name, Synonyms and Lineage

ICTV approved acronym: LTSV. Virus is an ICTV approved species of the genus 00.067.0.01. Sobemovirus; not assigned to a family.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 27-28 nm. Capsids appear hexagonal in outline. The capsomer arrangement is not obvious (ring-like subunits on some virions after freeze-dehydration and staining).

Electron microscopic preparation and references: Virus preparation contains few virions, or many virions. Reference for electron microscopic methods: Forster and Jones (1979).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.37 g cm-3. There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 112 S20w. The thermal inactivation point (TIP) is at 70°C. The longevity in vitro (LIV) is 28 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 5. The infectivity is lost when deproteinized with proteases; retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 18% of the virion by weight. The genome is monopartite. Only one particle size of linear, positive-sense, single-stranded RNA is recovered. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA and satellite RNA, that is mRNA including satellite RNA. The complete genome is 4230 nucleotides long, is fully or partially sequenced, complete sequence is 4230 nucleotides long. Sequence has the accession number

[D00341] Em(40)_vi:MTSCA Gb(84)_vi:MTSCA Lucerne transient streak virus(LTSV-C) RNA2, complete. 4/90 322bp.
[X01984] Em(40)_vi:LTSVARN2 Gb(84)_vi:LTSVARN2 Lucerne transient streak virus (LTSV-A) RNA 2 sequence. 7/93 324bp.
[X01985] Em(40)_vi:LTSVNRN2 Gb(84)_vi:LTSVNRN2 Lucerne transient streak virus (LTSV-N) RNA2 sequence. 7/93 324bp. 3 sequences.

The 5'-end of the genome has a genome-linked protein (VPg).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 82% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) (with a minor component that is probably a hydrolysis product of it).

Structural Proteins: Reference to method of preparation: Forster and Jones (1979).

Non-Structural Proteins: Virus-coded non-structural proteins have been identified by sequence analysis and 3 non-structural protein(s) are found.

Lipids

Lipids are not reported.

Transcription: Sub-genomic RNA is present in infected cells; encoding the coat protein (Mr 35000).

Translation: Virions may provide helper functions to dependent virus during replication. Virion acts as helper for a satellite RNA.

Antigenicity

The virus is serologically related to subterranean clover mottle virus, but distantly.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ROSIDAE.

Severity and Occurrence of Disease

Host: Signs and symptoms disappear soon after infection.

Transmission and Vector Relationships

Virus is transmitted by mechanical inoculation; not transmitted by seeds; not transmitted by pollen.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Chenopodiaceae, Compositae, Leguminosae-Papilionoideae, Solanaceae. The following species were susceptible to experimental virus infection: Chenopodium album, Chenopodium amaranticolor, Chenopodium quinoa, Cicer arietinum, Lupinus albus, Lupinus angustifolius, Medicago sativa, Medicago scutellata, Nicotiana clevelandii, Nicotiana rustica, Nicotiana tabacum, Petunia x hybrida, Pisum sativum, Spinacia oleracea, Trifolium incarnatum, Vicia faba, Vicia sativa, Zinnia elegans.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Caryophyllaceae, or Compositae, Cruciferae, Cucurbitaceae, Leguminosae-Papilionoideae, or Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Arachis hypogaea, Brassica campestris ssp. pekinensis, Calendula officinalis, Capsicum annuum, Capsicum frutescens, Crotalaria spectabilis, Cucumis sativus, Cucurbita maxima, Datura stramonium, Dianthus barbatus, Glycine max, Gomphrena globosa, Lablab purpureus, Lactuca sativa, Lotus corniculatus, Lycopersicon esculentum, Macroptilium lathyroides, Macrotyloma uniflorum, Nicotiana glutinosa, Nicotiana sylvestris, Phaseolus lunatus, Phaseolus vulgaris, Physalis floridana, Solanum melongena, Solanum nigrum, Trifolium pratense, Trifolium repens, Trifolium subterraneum, Vicia faba, Vigna angularis, Vigna unguiculata, Vigna unguiculata ssp. cylindrica.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium amaranticolor — necrotic local lesions; systemic chlorotic blotching (Australian, but not Ne Zealand isolates).

Chenopodium quinoa — necrotic and chlorotic local lesions; systemic mosaic.

Medicago scutellata — necrotic local lesions.

Pisum sativum — necrotic local lesions (New Zealand isolates), or symptomless (Australian isolates, no systemic infection.

Nicotiana clevelandii — chlorotic local lesions; systemic mosaic.

Diagnostic host: insusceptible host species Cucumis sativus, Gomphrena globosa, Phaseolus vulgaris, Nicotiana glutinosa, Vigna unguiculata.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Chenopodium quinoa, Nicotiana clevelandii.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium amaranticolor (L), C. quinoa (L), Pisum sativum (L).

References to host data: Blackstock (1978, Forster and Jones (1979).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm and cell vacuole.

Cytopathology: Inclusions are not present in infected cells.

Geographical Distribution

The virus occurs in Australia, Canada, and New Zealand (Aotearoa).

References

Blackstock, J.M. (1974). Aust. Pl. Path. Soc. Newsl. 3: 6.

Blackstock, J.M. (1978). Aust. J. agric. Res. 29: 291.

Forster, R.L.S. and Jones, AT (1979). Ann. appl. Biol. 93: 181.

Forster, R.L.S. and Jones, AT (1980). CMI/AAB Descr. Pl. Viruses No. 224, 4 pp.

Jones, AT, Mayo, MA and Duncan GH (1983). J. gen. Virol. 64: 1167.

Morris-Krsinich, B.A.M., Forster, R.L.S. and Mossop, D.W. (1983). Virology 124: 349.

Tien, P., Davies, C., Hatta, T. and Francki, RIB. (1981). FEBS Lett. 132: 353.

The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 457 by R.L.S. Forster, 1982. Revised 1983.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 224.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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