[Home] [ICTV Taxonomy - Index of Viruses] [Virus Descriptions] [Character List] [Picture Gallery]
[Tutorial] [Online Data Retrieval & Identification] [Virus Isolate Registration & Submission] [Search]
Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

00.060.0.09.001. Rice ragged stunt virus

Cite this publication as: ICTVdB Management (2006). 00.060.0.09.001. Rice ragged stunt virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Indonesia (and the Philippines).

Host of Isolate and Habitat Details
Source of isolate: Oryza sativa.

Natural host and symptoms
Oryza latifolia, O. nivara, O. sativa — stunting, enations on veins of leaves and leaf sheaths, ragged leaves, flower suppression.

Reference to Isolation Report
Hibino et al. (1977, Ling (1977, Shikata et al. (1977).

Classification

This is a description of a plant and invertebrate (viruses replicate in both hosts and are transmitted by viruliferous planthoppers to Poacea) virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.060.0.09.001. Virus accession number: 60009001. Obsolete virus code: 60.0.9.0.001; superceded accession number: 60090001.
NCBI Taxon Identifier NCBI Taxonomy ID: 42475.

Name, Synonyms and Lineage

Synonym(s): rice infectious gall virus. ICTV approved acronym: RRSV. Virus is the type species. Virus is of the genus 00.060.0.09. Oryzavirus in the family 00.060. Reoviridae.

Virion Properties

Morphology

Virions consist of a capsid, a core, and a nucleoprotein complex. Virus capsid is not enveloped. Capsid/nucleocapsid is round and exhibits icosahedral symmetry. The capsid is isometric. The capsid shells of virions are composed of a single inner capsid layer. The outer shell is not present. Capsids appear round. The capsid surface structure reveals a regular pattern with distinctive features. The capsomer arrangement is not obvious (but with 12 "B" type spikes 8-10 nm long). Surface projections are distinct "B"-type spikes protruding from the 12 vertices. The spikes are 8-10 nm long, 23-26 nm wide at the base and 14-17 nm at the top, that overlie the core). Inner capsids consist of a 65 nm. The core subcore is spherical (and smooth with no spikes) with a diameter of 50 nm.

Only one species is recovered in preparations.

Electron microscopic preparation and references: Virus preparation contains few virions (whole leaf sap), or many virions (in sap from enations). Reference for electron microscopic methods: Milne et al. (1982).

Physicochemical and Physical Properties

There are 1 sedimenting component(s) found in purified preparations.

Nucleic Acid

The genome is segmented and consists of ten segments of linear double-stranded RNA. Minor species of non-genomic nucleic acid are not found in virions. The complete genome is 26660 nucleotides long, is sequenced and complete sequence is about 3900 nucleotides long, is sequenced, but only an estimate is available, complete sequence is 3900 nucleotides long.   Is sequenced, but only an estimate is given, complete sequence is 3800 nucleotides long, has been sequenced, but only an estimate is provided; complete sequence is 3800 nucleotides long and has been sequenced, but only an estimate is presented, complete sequence is 2750 nucleotides long. RNA-6 has been sequenced, complete sequence is 2300 nucleotides long, has been sequenced, but only an estimate is presented; complete sequence is 1950 nucleotides long. RNA-8 has been sequenced, but only an estimate is available, complete sequence is 1900 nucleotides long. RNA-9 has been sequenced, complete sequence is about 1200 nucleotides long. RNA-10 has been sequenced, but only an estimate is available; complete sequence is 1160 nucleotides long. The 5'-terminal sequence has conserved regions; terminal repeats at the 5'-end are 6 nucleotides long (5' GAUAAA....GUGC 3'). The 3'-terminus has conserved nucleotide sequences; of 4 nucleotides in length; sequence has conserved regions; in some RNA segments (but different from those of phytoreoviruses or Fijiviruses).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 8 structural protein(s).

Lipids

Lipids are absent.

Genome Organization and Replication

By itself, genomic nucleic acid is not infectious; a successful infection by the virus requires the intact virion.

Translation: Coat protein mRNA is translated in the cytoplasm.

The genome replicates in cytoplasmic viroplasma.

Antigenicity

The virus does not show serological relationships to sugarcane Fiji disease, maize rough dwarf, pangola stunt, rice black-streaked dwarf, oat sterile dwarf, rice dwarf, rice gall dwarf and clover wound tumor viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is not transmitted by mechanical inoculation; not transmitted by contact between hosts; not transmitted by seeds; not transmitted by pollen.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Hemiptera, family Delphacidae; Nilaparvata lugens. Virus is transmitted in a persistent manner; retained when the vector moults; replicates in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Gramineae. The following species were susceptible to experimental virus infection: Hordeum vulgare, Oryza latifolia, Oryza nivara, Oryza sativa, Zea mays.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Gramineae. Species inoculated with virus that do not show signs of susceptibility: Saccharum officinarum, Triticum aestivum.

Diagnostic Hosts

Diagnostic host species and symptoms:

Oryza sativa cv. Taichung Native (TN-1) — pale spindle-shaped enations on abaxial surfaces of leaves and leaf sheaths, twisted and ragged leaves, dark green colour, suppressed flowering, infected plants remain green and appear grassy after healthy plants have senesced. Diagnostic host: insusceptible host species Triticum aestivum, Saccharum spp.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Oryza sativa cv. Taichung Native (TN-1).

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Oryza sativa (W).

References to host data: Hibino (1979, Shikata et al. (1979).

Histopathology: Virus can be best detected in phloem enations. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are viroplasma. Inclusions contain mature virions. Other cellular changes include cell enlargement and sometimes cell wall proliferation.

Geographical Distribution

The virus occurs in Bangladesh, China, India, Indonesia, Japan, Malaysia, the Philippines, Sri Lanka, Taiwan, and Thailand.

References

Boccardo, G. and Milne, R.G. (1980). Intervirology 14: 57.

Boccardo, G. and Milne, R.G. (1984). CMI/AAB Descr. Pl. Viruses No. 294, 7 pp.

Chen, C.C., Hsu, Y.H., Chen, M.J., and Chin, R.J. (1989). Intervirology 30: 278.

Hibino, H. (1979). Rev. Pl. Protect. Res. 12: 98.

Hibino, H. and Kimura, I. (1982). Phytopathology 72: 656.

Hibino, H., Saleh, N. and Roechan, M. (1979). Ann. Phytopath. Soc. Japan 45: 228.

Hibino, H., Roechan, M., Sudarisman, S. and Tantera, DM (1977). Contr. Centr. Res. Inst. Agric. Bogor No. 35: 15.

Lee, S.Y., Uyeda, I. and Shikata, E. (1987). intervirology 27: 189.

Ling, K.C. (1977). Int. Rice Res. Newsl. 2: 6.

Ling, K.C., Tiongco, E.R., Aguiero, V.M. and Cabauatan, P.Q. (1978). Int. Rice Res. Paper Series 16, 25 pp.

Luisoni, E., Milne, R.G. and Roggero, P. (1982). Plant Dis. 66: 929.

Milne, R.G. (1980). Intervirology 14: 331.

Milne, R.G., Luisoni, E. and Ling, K.C. (1979). Pl. Dis. Reptr 63: 445.

Milne, R.G., Boccardo, G. and Ling, K.C. (1982). CMI/AAB Descr. Pl. Viruses No. 248, 5 pp.

Shikata, E., Senboku, T., Kamjaipai, K., Chou, T., Tiongco, E.R. and Ling, K.C. (1979). Ann. Phytopath. Soc. Japan 45: 436.

Shikata, E., Leelopang, Tiongo, E.R. and Ling, K.C. (1977). Int. Rice Res. Newsl. 2: 7.

Yan, J., Kudo, H., Uyeda, I., Lee, S.Y. and Shikata, E. (1992). J. gen. Virol. 73: 785. The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 691 by G. Boccardo, 1984. A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 248.



Limit search to: Title & Body Title Document Path
Show Reverse Sort

DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

ICTVdB and DELTA related References

Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
Copyright © 2002    International Committee on Taxonomy of Viruses.    All rights reserved.


Additional access points to virus species lists, descriptions and images on the web:

Species catalogue                     iSpecies.org - a species search engine           a species search engine

Google Analytics      Google Analytics: activity view