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00.060.0.07.007. Rice black streaked dwarf virus

Cite this publication as: ICTVdB Management (2006). 00.060.0.07.007. Rice black streaked dwarf virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Japan.

Host of Isolate and Habitat Details
Source of isolate: Oryza sativa var. japonica.

Natural host and symptoms
Oryza sativa, Zea mays, Triticum sativum, Avena sativa, Hordeum vulgare — stunted, deformed leaves with white waxy or black-streaked swellings along the veins.

Reference to Isolation Report
Kuribayashi and Shinkai (1952).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.060.0.07.007. Virus accession number: 60007008. Obsolete virus code: 60.0.7.2.003; superceded accession number: 60072003.
NCBI Taxon Identifier NCBI Taxonomy ID: 10990.

Name, Synonyms and Lineage

Synonym(s): rice black streak virus. ICTV approved acronym: RBSDV. Virus is an ICTV approved species of the genus 00.060.0.07. Fijivirus; family 00.060. Reoviridae.

Virion Properties

Morphology

Virions consist of a capsid, a core, and a nucleoprotein complex. Virus capsid is not enveloped. Capsid/nucleocapsid is isometric with icosahedral symmetry and has a diameter of 75-80 nm (in cells of infected plants and insects), or 70 nm (intact virions). The capsid shells of virions are composed of two layers. The outer capsid consists of a smooth (showing A-type spikes of about 11nm length and breadth). All shells are usually present. Capsids appear hexagonal in outline. The capsomer arrangement is not obvious. Surface projections are distinct spikes protruding from the 12 vertices. Inner capsids consist of a 55 nm consists of a rough shell (showing B-type spikes, about 8 nm long and 12 nm in diameter). The core subcore is spherical (and smooth with no spikes) with a diameter of 35-40 nm.

Electron microscopic preparation and references: Virus preparation contains many virions. Leaves fixed in 2% osmium tetroxide or 2% paraformaldehyde before extraction yield the large virions of 75-80 nm with the outer projections attached. Reference for electron microscopic methods: Kitagawa and Shikata (1969, Kawano et al. (1984).

Physicochemical and Physical Properties

The thermal inactivation point (TIP) is at 60°C. The longevity in vitro (LIV) is 6 days, or 7 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 5 (in rice plants), or 6 (in insects).

Nucleic Acid

The genome is segmented and consists of ten segments of linear double-stranded RNA. The complete genome is 23898 nucleotides long. RNA-1 is fully sequenced and complete sequence is 3998 nucleotides long. Sequence has the accession number

[D00606] Em(40)_vi:RBDSEG10 Gb(84)_vi:RBDSEG10 Rice black-streaked dwarf virus genome segment 10. 11/90 1,801bp.
[L36524] Gb(84)n:RBDGS3A Rice black-streaked dwarf virus genome segment 3 fragment. 10/94 939bp.
[S63914] Em(40)_vi:S63914 Gb(84)_vi:S63914 orf (segment 8) rice black-streaked dwarf virus RBSDV, Genomic RNA, 1927 nt. 1/94 1,927bp.
[S63917] Em(40)_vi:S63917 Gb(84)_vi:S63917 orf1, orf2 (segment 7) rice black-streaked dwarf virus RBSDV, Genomic RNA, 2193 nt. 1/94 2. RNA-2 is sequenced, complete sequence is about 3385 nucleotides long. RNA-3 is sequenced, complete sequence is 3310 nucleotides long. RNA-4 has been sequenced, but only an estimate is provided; complete sequence is 3310 nucleotides long. RNA-5 has been sequenced, but only an estimate is presented, complete sequence is 3100 nucleotides long. RNA-6 has been fully sequenced. Complete sequence is 2450 nucleotides long. RNA-7 has been sequenced, but only an estimate is presented, complete sequence is 2198 nucleotides long. RNA-8 has been sequenced, but only an estimate is available, complete sequence is 1830 nucleotides long. RNA-9 has been sequenced and complete sequence is about 1826 nucleotides long. RNA-10 has been sequenced, but only an estimate is available; complete sequence is 1810 nucleotides long.

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins.

Lipids

Lipids are absent.

Antigenicity

Reported to be serologically closely related to maize rough dwarf virus (Luisoni et al., 1973, their relationship is so close that they may be the same species.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is not transmitted by mechanical inoculation; not transmitted by seeds.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Hemiptera, family Delphacidae; Laodelphax striatellus (Kuribayashi and Shinkai, 1952; Shinkai, 1962), Unkanodes sapporona (Shinkai, 1966) and U. albifascia (Shinkai, 1967). Virus is transmitted in a persistent manner; retained when the vector moults; replicates in the vector; not transmitted congenitally to the progeny of the vector.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Gramineae. The following species were susceptible to experimental virus infection: Avena sativa, Hordeum vulgare, Oryza sativa, Oryza sativa var. japonica, Triticum aestivum, Triticum sativum, Zea mays.

Diagnostic Hosts

Diagnostic host species and symptoms:

Oryza sativa var. japonica — stunting, darkening of leaves, twisting of tips of young leaves, white waxy swellings along veins on the abaxial leaf surface, later becoming brown and forming black-streaked tumours.

Zea mays — dwarfening, darkening of leaves, white streaks along the veins and white waxy swollen veins on the abaxial surface of leaves.

Triticum sativum, Avena sativa, Hordeum vulgare — severe stunting, twisting of leaves, sometimes with waxy swollen veins on the abaxial surface of leaves and culms.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Oryza sativa, Triticum sativum, Avena sativa and Hordeum vulgare.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Zea mays cv. Golden Cross Bantam (W) (Zea mays useless as a virus source for insect transmission).

References to host data: Shinkai (1962, Morinaka and Sakurai (1968).

Histopathology: Virus can be best detected in phloem. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm and nucleus. Nuclear inclusion bodies are crystals. Cytoplasmic inclusions are viroplasma. Inclusions contain mature virions. Other cellular changes include hyperplasia and hypertrophy of phloem cells.

Geographical Distribution

The virus occurs in China, Japan, Korea (North), and Korea (South).

References

Kashiwagi, Y. (1966). Ann. Phytopath. Soc. Japan 32: 168.

Kawano, S., Uyeda, I. and Shikata, E. (1984). J. Fac. Agric. Hokkaido Univ. 61: 408.

Kitagawa, Y. and Shikata, E. (1969). Mem. Fac. Agric. Hokkaido Univ. 6: 446.

Kuribayashi, K. and Shinkai, A (1952). Ann. Phytopath. Soc. Japan 16: 41.

Luisoni, E., Lovisolo, O., Kitagawa, Y. and Shikata, E. (1973). Virology 52: 281.

Morinaka, T. and Sakurai, Y. (1968). Bull. Chugoku agric. exp. Stn. Ser. E 2: 1.

Shikata, E. (1974). CMI/AAB Descr. Pl. Viruses No. 135, 4 pp.

Shinkai, A (1962). Bull. nAT Inst. agric. Sci., Tokyo Ser. C., p. 223.

Shinkai, A (1966). Ann. Phytopath. Soc. Japan 32: 317.

Shinkai, A (1967). Ann. Phytopath. Soc. Japan 35: 318.

The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 684 by E. Shikata, 1987; Li Jing, 1987.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 135.



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ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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