[Home] [ICTV Taxonomy - Index of Viruses] [Virus Descriptions] [Character List] [Picture Gallery]
[Tutorial] [Online Data Retrieval & Identification] [Virus Isolate Registration & Submission] [Search]
Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

00.056.0.01.019. Tulip virus X

Cite this publication as: ICTVdB Management (2006). 00.056.0.01.019. Tulip virus X. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: the United Kingdom.

Host of Isolate and Habitat Details
Source of isolate: Tulipa sp.

Natural host and symptoms
Tulipa ssp. — chlorotic or necrotic grey-brown streaking of leaves; streaks of intensified pigment (or of necrosis) in petals.

Reference to Isolation Report
Mowat (1982).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.056.0.01.019. Virus accession number: 56001019. Obsolete virus code: 56.0.1.0.019; superceded accession number: 56010019.
NCBI Taxon Identifier NCBI Taxonomy ID: 167132.

Name, Synonyms and Lineage

ICTV approved acronym: TVX. Virus is an ICTV approved species of the genus 00.056.0.01. Potexvirus in the family 00.056. Flexiviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. The capsid is filamentous, flexuous with a length of 495 nm and a width of c. 13 nm. Axial canal is indistinct. Basic helix is obscure. Pitch of helix is 3.25 nm.

Electron microscopic preparation and references: Virus preparation contains many virions. Reference for electron microscopic methods: Mowat (1982).

Physicochemical and Physical Properties

There are 2 sedimenting component(s) found in purified preparations. The sedimentation coefficient is of the fastest 118 S20w; of the other(s) are 102 S20w. The thermal inactivation point (TIP) is at 60-65°C. The longevity in vitro (LIV) is 30 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 9.

Nucleic Acid

The Mr of the genome constitutes 8% of the virion by weight. The genome is monopartite, only one particle size is recovered of linear, positive-sense, single-stranded RNA. The genome is fully sequenced and complete sequence is 6459 nucleotides long. Reference to nucleotide sequence isolation method by Mowat (1982): two cycles of precipitation with 8% polyethylene glycol (Mr 6000) in the presence of 0.2M NaCl.

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 92% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s).

Lipids

Lipids are not reported.

Transcription: The virus codes for 5 ORF(s).

Coding Strategy of Segment 1: Sequence has a gene block. Sequence has triple gene block sequence (TGB). Encodes proteins involved in cell to cell movement.

Antigenicity

The virus is serologically related to hydrangea ringspot and viola mottle viruses. The virus does not show serological relationships to bamboo mosaic, cactus X, clover yellow mosaic, foxtail mosaic, lily X, narcissus mosaic, nerine X, plantain X and potato X viruses.

Diagnostics and Reference Collections

The best tests for diagnosis are symptoms in tulip resemble those caused by other viruses, but systemic line patterns in Chenopodium amaranticolor and the failure to infect Nicotiana clevelandii, combined with the presence of potexvirus virions, give a reliable diagnosis, especially when confirmed by serological tests.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

General Symptoms in Plants Symptoms chlorotic or necrotic grey-brown streaking of leaves; streaks of intensified pigment (or of necrosis) in petals.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is not transmitted by a vector; manual inoculation of tulips is only occasionally successful, thus spread by contact between plants may be rare. Virus is transmitted by mechanical inoculation; not transmitted by contact between hosts (probably).

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in many families. Susceptible host species are found in the Family Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Leguminosae-Papilionoideae, Liliaceae, Solanaceae, Tetragoniaceae, Umbelliferae, Violaceae (1 /1). The following species were susceptible to experimental virus infection: Anthriscus cerefolium, Apium graveolens, Beta vulgaris, Chenopodium amaranticolor, Chenopodium quinoa, Coriandrum sativum, Cucumis sativus, Daucus carota, Gomphrena globosa, Heracleum sphondylium, Lycopersicon esculentum, Nicotiana benthamiana, Petroselinum crispum, Spinacia oleracea, Tetragonia tetragonioides, Trifolium incarnatum, Tulipa, Viola odorata.

Host:
Experimentally infected hosts mainly show symptoms of chlorotic local lesions and chlorotic mottle.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Compositae, Cruciferae, Gramineae, or Leguminosae-Papilionoideae, Liliaceae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Brassica campestris, Brassica campestris ssp. rapa, Capsicum annuum, Cyamopsis tetragonoloba, Datura stramonium, Lactuca sativa, Lilium formosanum, Nicotiana clevelandii, Nicotiana debneyi, Nicotiana glutinosa, Nicotiana rustica, Nicotiana tabacum, Petunia x hybrida, Phaseolus vulgaris, Pisum sativum, Setaria italica, Trifolium pratense, Trifolium repens, Vicia faba.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium amaranticolor, C. quinoa, Trifolium incarnatum — chlorotic local lesions; systemic chlorotic mottle.

Spinacia oleracea, Nicotiana benthamiana, Apium graveolens, Viola odorata — symptomless systemic infection. Diagnostic host: insusceptible host species Brassica campestris, Trifolium pratense, Nicotiana clevelandii, N. tabacum cv. White Burley.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Chenopodium amaranticolor, C. quinoa.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium amaranticolor (L).

References to host data: Mowat (1982).

Histopathology: Virus can be best detected in epidermal cells of leaves.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Inclusions are spindle shaped. Inclusions contain mature virions.

Geographical Distribution

The virus is found, but with no evidence of proliferation, in the U.K. and Sweden.

References

Mowat, W.P. (1982). Ann. appl. Biol. 101: 51.

Mowat, W.P. (1984). CMI/AAB Descr. Pl. Viruses No. 276, 4pp.

The following generic references are cited in the most recent ICTV Report.

PubMed References. A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 853 by W.P. Mowat, 1990. A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 276.



Limit search to: Title & Body Title Document Path
Show Reverse Sort

DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

ICTVdB and DELTA related References

Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
Copyright © 2002    International Committee on Taxonomy of Viruses.    All rights reserved.


Additional access points to virus species lists, descriptions and images on the web:

Species catalogue                     iSpecies.org - a species search engine           a species search engine

Google Analytics      Google Analytics: activity view