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00.056.0.01.011. Narcissus mosaic virus

Cite this publication as: ICTVdB Management (2006). 00.056.0.01.011. Narcissus mosaic virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: the Netherlands (and the U.K).

Host of Isolate and Habitat Details
Source of isolate: Narcissus pseudonarcissus.

Natural host and symptoms
Narcissus pseudonarcissus — symptomless infection.

Reference to Isolation Report
Van Slogteren and de Bruyn Ouboter (1946, Brunt (1966).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.056.0.01.011. Virus accession number: 56001011. Obsolete virus code: 56.0.1.0.011; superceded accession number: 56010011.
NCBI Taxon Identifier NCBI Taxonomy ID: 12180.

Name, Synonyms and Lineage

ICTV approved acronym: NMV. Virus is an ICTV approved species of the genus 00.056.0.01. Potexvirus in the family 00.056. Flexiviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. The capsid is filamentous, flexuous with a length of 550 nm and a width of 13-14 nm. Axial canal is indistinct. Basic helix is obscure. Pitch of helix is 3.4 nm.

Electron microscopic preparation and references: Virus preparation contains many virions. Reference for electron microscopic methods: Mowat (1971).

Physicochemical and Physical Properties

There are 2 sedimenting component(s) found in purified preparations. The sedimentation coefficient is of the fastest, the 550 nm virions 114 S20w. The thermal inactivation point (TIP) is at 75°C. The longevity in vitro (LIV) is 85 days (and more). Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 6. The infectivity is not changed by treatment with ether.

Nucleic Acid

The Mr of the genome constitutes 6% of the virion by weight. The genome is monopartite, only one particle size is recovered of linear, positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA that is mRNA. The complete genome is 6955 nucleotides long, is fully sequenced and partially sequenced, complete sequence is 6955 nucleotides long. Sequence has the accession number

[D00405] Em(40)_vi:NMV Narcissus mosaic virus (NMV), complete genome. 8/90 6,955bp
[D13747] Em(40)_vi:MNCCGAA Gb(84)_vi:MNCCGAA Narcissus mosaic virus (NMV), complete genome. 11/92 6,955bp. 2 sequences. Reference to nucleotide sequence Short and Davies (1983).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 94% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s).

Structural Proteins: Reference to method of preparation: Robinson et al. (1975).

Reference to amino acid sequence or composition Short (1982).

Non-Structural Proteins: Virus-coded non-structural proteins have been identified by sequence analysis and 3 non-structural protein(s) are found.

Lipids

Lipids are not reported.

Transcription: The virus codes for 5 ORF(s).

Sub-genomic RNA is present in infected cells.

Coding Strategy of Segment 1: Sequence has a gene block. Sequence has triple gene block sequence (TGB). Encodes proteins involved in cell to cell movement.

Antigenicity

The virus does not show serological relationships to tulip Virus X.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

Transmission and Vector Relationships

Virus is transmitted by mechanical inoculation; not transmitted by contact between hosts (between narcissus plants, not transmitted by seeds.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Amaranthaceae, Amaryllidaceae, Apocynaceae, Chenopodiaceae, Compositae, Leguminosae-Papilionoideae, Solanaceae, Tetragoniaceae, Umbelliferae. The following species were susceptible to experimental virus infection: Apium graveolens, Atriplex hortensis, Catharanthus roseus, Chenopodium amaranticolor, Chenopodium quinoa, Datura stramonium, Glycine max, Gomphrena globosa, Lactuca sativa, Medicago sativa, Narcissus pseudonarcissus, Nerine bowdenii, Nicandra physalodes, Nicotiana clevelandii, Petunia x hybrida, Pisum sativum, Tetragonia tetragonioides, Trifolium pratense, Trifolium repens.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Alliaceae, Chenopodiaceae, Cruciferae, or Cucurbitaceae, Gramineae, Leguminosae-Papilionoideae, Liliaceae, or Polygonaceae, Scrophulariaceae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Allium cepa, Antirrhinum majus, Beta vulgaris, Brassica campestris ssp. pekinensis, Brassica juncea, Cucumis sativus, Fagopyrum esculentum, Lilium formosanum, Lycopersicon esculentum, Nicotiana glutinosa, Nicotiana rustica, Nicotiana tabacum, Phaseolus vulgaris, Solanum tuberosum, Vicia faba, Zea mays.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium amaranticolor — chlorotic local lesions.

Nicotiana clevelandii — symptomless systemic infection.

Gomphrena globosa — necrotic local lesions. Diagnostic host: insusceptible host species Nicotiana tabacum cv. White Burley, N. glutinosa, Brassica campestris ssp. pekinensis.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Chenopodium quinoa, Nicotiana clevelandii (not infected by some isolates).

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium amaranticolor (L).

References to host data: Brunt (1966).

Histopathology: Virus can be best detected in epidermis. Virions are found in the cytoplasm, nucleus, and cell vacuole.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Inclusions are spindle-shaped. Inclusions contain mature virions.

Geographical Distribution

The virus is probably distributed worldwide (in all countries which have imported narcissus and nerine from Europe). The virus occurs in particularly the Netherlands and the United Kingdom.

List of Strains and Isolates in the Species

Lychnis virus (RI Hamilton in Koenig, 1985).

References

Bancroft, J.B., Hills, G.J. and Richardson, J.F. (1980). J. gen. Virol. 50: 451.

Brunt, A.A. (1966). Ann. appl. Biol. 58: 13.

Koenig, R. (1985). Acta Hort. 164: 21.

Mowat, W.P. (1971). CMI/AAB Descr. Pl. Viruses No. 45, 3 pp.

Robinson, D.J., Hutcheson, A, Tollin, P. and Wilson, H.R. (1975). J. gen. Virol. 29: 325.

Short, M.N. (1982). Ph.D. Thesis, University of East Anglia, U.K.

Short, M.N. and Davis, J.W. (1983). Bioscience Rep. 3: 837.

Van Slogteren, D.H.M. and de Bruyn Ouboter, M.P. (1946). Daffodil Tulip Yrbk. 12: 3.

Zuidema, D., Linthorst, H.J.M., Huisman, M.J., Asjes, C.J. and Bol, J.F. (1989). J. gen. Virol. 70: 267.

The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 511 by W.P. Mowat, 1986. A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 45.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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