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00.049.0.04.001. White clover cryptic virus 2

Cite this publication as: ICTVdB Management (2006). 00.049.0.04.001. White clover cryptic virus 2. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Italy.

Host of Isolate and Habitat Details
Source of isolate: Trifolium repens.

Natural host and symptoms
Trifolium repens — no symptoms.

Reference to Isolation Report
Boccardo et al. (1985).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.049.0.04.001. Virus accession number: 49004001. Obsolete virus code: 49.0.4.0.001; superceded accession number: 49040001.

Name, Synonyms and Lineage

ICTV approved acronym: WCCV-2. Virus is the type of the genus 00.049.0.04. Betacryptovirus in the family 00.049. Partitiviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The nucleocapsid is isometric and has a diameter of 38 nm. Capsids appear round. The capsomer arrangement is clearly visible.

Electron microscopic preparation and references: Reference for electron microscopic methods: Boccardo et al. (1985).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.375 g cm-3. There are 1 sedimenting component(s) found in purified preparations. A260/A280 ratio is 1.31.

Nucleic Acid

The Mr of the genome constitutes 24% of the virion by weight. The genome is segmented; bipartite, segements are distributed among 2 particle types of linear, double-stranded RNA. The complete genome is 4310 nucleotides long, is sequenced and complete sequence is about 2238 nucleotides long, is sequenced, but only an estimate is available, complete sequence is 2072 nucleotides long. The multipartite genome is possible packaged in one particle, but it is not known whether they are packaged together or separately.

Proteins

Proteins constitute about 76% of the particle weight.

The viral genome encodes structural proteins.

Lipids

Lipids are not reported.

Antigenicity

The virus is serologically related to red clover 2 and hop trefoil 2 betacryptoviruses.

Complementary cDNA probes to white clover 2 virus failed to detect any homology with white clover 1 and 3 alphacryptoviruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Transmission and Vector Relationships

Virus is not transmitted by mechanical inoculation; not transmitted by grafting; transmitted by seeds.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Leguminosae-Papilionoideae. The following species were susceptible to experimental virus infection: Trifolium repens.

Diagnostic Hosts

Diagnostic host species and symptoms:

none known.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Trifolium repens containing no other viruses.

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm.

Cytopathology: Inclusions are not present in infected cells.

Geographical Distribution

The virus is found, but with no evidence of proliferation, in Europe, Japan, New Zealand and the U.S.A.

Comments

This virus was originally described as white clover temperate virus (Natsuaki et al., 1984), and was shown later to be a mixture of white clover cryptic viruses 1, 2 and 3 (Natsuaki et al., 1986).

References

Boccardo, G., Lisa, V. and Milne, R.G. (1983). In: Double-Stranded RNA Viruses, p. 425; eds R.W. Compans and D.H.L. Bishop. Elsevier, New York.

Boccardo, G., Milne, R.G., Luisoni, E., Lisa, V. and Accotto, G.P. (1985). Virology 147: 29.

Boccardo, G., Lisa, V., Luisoni, E. and Milne, R.G. (1987). Adv. Virus Res. 32: 171.

Luisoni, E. and Milne, R.G. (1988). AAB Descr. Pl. Viruses No. 332, 4 pp.

Luisoni, E., Milne, R.G., Accotto, G.P. and Boccardo, G. (1987). Intervirology 28: 144.

Natsuaki, K.T., Natsuaki, T., Okuda, S., Teranaka, M., Yamashita, S. and Doi, Y. (1984). J. agric. Sci., Tokyo Nogyo Daigaku 29: 49.

Natsuaki, T., Natsuaki, K.T., Okuda, S., Teranaka, M., Milne, R.G., Boccardo, G. and Luisoni, E. (1986). Intervirology 25: 69.
The following generic references are cited in the most recent ICTV Report.
A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 894 by A.A. Brunt, 1991. A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 332.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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