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00.029.0.03.001. Bean golden yellow mosaic virus

Cite this publication as: ICTVdB Management (2006). 00.029.0.03.001. Bean golden yellow mosaic virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Costa Rica.

Host of Isolate and Habitat Details
Source of isolate: Phaseolus vulgaris.

Natural host and symptoms
Macroptilium lathyroides, Phaseolus vulgaris, P. lunatus, Malvastrum coromandelianum — vein yellow net, interveinal chlorosis.

Reference to Isolation Report
Galvez and Castano (1976).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.029.0.03.001. Virus accession number: 29003001. Obsolete virus code: 29.0.3.0.001; superceded accession number: 29030001.
NCBI Taxon Identifier NCBI Taxonomy ID: 222448.

Name, Synonyms and Lineage

Synonym(s): virus del mosaico dorado; Bean golden mosaic virus - Puerto Rico. ICTV approved acronym: BGMV. Virus is the type species of the genus 00.029.0.03. Begomovirus in the family 00.029. Geminiviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid is elongated and exhibits icosahedral symmetry. The capsid is geminate and has a diameter of 18-20 nm. Capsids appear hexagonal in outline. The capsomer arrangement is not obvious. The capsid consists of 22 capsomers. With a length of dimers 30 nm.






















Electron micrograph of Geminiviridae by R.G. Milne, Istituto di Virologia, CRN, Torino, Italy.

Electron microscopic preparation and references: Virus preparation contains few virions. Aldehyde fixation essential unless PTA used at pH 4. Reference for electron microscopic methods: Goodman et al. (1977, Goodman and Bird (1978).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.717 g cm-3 (without aldehyde fixation). The density of virions is 1.31 in Cs2SO4. There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 69 S20w. Isoelectric point pH is 7. The thermal inactivation point (TIP) is at 50-55°C. The longevity in vitro (LIV) is 72 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 2. The infectivity is decreased when deproteinized with proteases (proteinase K has no effect on protoplast infectivity, retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 20% of the virion by weight. The genome is segmented and consists of two segments of (similar,) circular, ambisense, single-stranded DNA ; that forms a closed circle. Minor species of non-genomic nucleic acid are not found in virions. The complete genome is 5233 nucleotides long. The DNA-1 is fully sequenced, complete sequence is 2646 nucleotides long. Sequence has the accession number
[D00200] Em(40)_vi:GEMBGACG Gb(84)_vi:MBGACG Bean golden mosaic virus (BGMV), DNA A, complete sequence. 4/90 2,585bp
[L01635] Em(40)_vi:GEMBGCOAR Gb(84)_vi:MBGCOAREPA Bean golden mosaic virus type II coat protein (AR1) gene complete cds, (AL2 and AL3)
[L01636] Em(40)_vi:GEMBGCOA1 Gb(84)_vi:MBGCOAREPB Bean golden mosaic virus type II (BR1 and BL1) genes, complete cds. 11/92 2,608bp.
[L34266] Gb(84)_vi:MBGAC1A Bean golden mosaic virus (clone pCR1) AC1 gene, partial cds. 7/94 222bp.
[L34267] Gb(84)_vi:MBGAC1B Bean golden mosaic virus (clone pCR1) AC1 gene, partial cds. 7/94 222bp.
[L34268] Gb(84)_vi:MBGAC1C Bean golden mosaic virus (clone pGA3) AC1 gene, partial cds. 7/94 222bp.
[L34269] Gb(84)_vi:MBGAC1D Bean golden mosaic virus (clone pJA1) AC1 gene, partial cds. 7/94 222bp
[M10070] Em(40)_vi:GEBGMV1 Gb(84)_vi:MBGCG1Z Bean golden mosaic virus, DNA 1 of complete genome. 1/91 2,646bp.
[M10080] Em(40)_vi:GEBGMV2 Gb(84)_vi:MBGCG2Z Bean golden mosaic virus, DNA 2 of complete genome. 4/90 2,587bp.
[M88686] Em(40)_vi:GEMBGARAL Gb(84)_vi:MBGARAL Bean golden mosaic virus viral coat protein (AR1) gene, complete cds; putative replicat
[M88687] Em(40)_vi:GEMBGBRBL Gb(84)_vi:MBGBRBL Bean golden mosaic virus BR1 gene, complete cds and BL1 gene, complete cds. 9/92 2,580b
[M91604] Em(40)_vi:GEMBGCGA Gb(84)_vi:MBGCGA Bean golden mosaic virus coat protein gene, complete cds; putative replication protein gene
[M91605] Em(40)_vi:GEMBGCGB Gb(84)_vi:MBGCGB Bean golden mosaic virus BR1 and BL1 genes, complete cds's. 9/92 2,596bp.
[S66724] Em(40)_vi:S66724 Gb(84)_vi:S66724 (clone DR-1 hypervariable region) bean golden mosaic virus, Genomic, 203 nt. 1/94 203
[S66726] Em(40)_vi:S66726 Gb(84)_vi:S66726 (clone DR-2 hypervariable region) bean golden mosaic virus, Genomic, 203 nt. 1/94 203
[S66729] Em(40)_vi:S66729 Gb(84)_vi:S66729 (clone DR-3 hypervariable region) bean golden mosaic virus, Genomic, 203 nt. 1/94 203
[S66730] Em(40)_vi:S66730 Gb(84)_vi:S66730 (clone DR-4 hypervariable region) bean golden mosaic virus, Genomic, 203 nt. 1/94 203
[S66732] Em(40)_vi:S66732 Gb(84)_vi:S66732 (clone DR-5 hypervariable region) bean golden mosaic virus, Genomic, 203 nt. 1/94 203
[S66733] Em(40)_vi:S66733 Gb(84)_vi:S66733 (DNA-B, clone pDRB-PCR hypervariable region) bean golden mosaic virus, Genomic, 203 nt.
DNA-B is fully sequenced, complete sequence is 2587 nucleotides long and has the accession number
[D00201] Em(40)_vi:GEMBGBCG Gb(84)_vi:MBGBCG Bean golden mosaic virus (BGMV), DNA B, complete genome. 1/91 2,647bp. The genome has a base ratio of 21 % guanine; 28.1 % adenine; 18.3 % cytosine; 32.6 % thymidine. Reference to nucleotide sequence Howarth et al. (1985).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 80% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s).

Structural Proteins: Reference to method of preparation: Goodman et al. (1980).

Reference to amino acid sequence or composition inferred from nucleotide sequence.

Non-Structural Proteins: Virus-coded non-structural proteins have been identified by sequence analysis and 5 non-structural protein(s) are found.

Lipids

Lipids are not reported.

Genome Organization and Replication

By itself, genomic nucleic acid is infectious (both DNAs required for full systemic infection).

Translation: Coat protein mRNA is translated in the cytoplasm.

The genome replicates in probably the nucleus.

Antigenicity

The virus is serologically related to Abutilon mosaic, African cassava mosaic, squash leaf curl and tomato golden mosaic viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ROSIDAE; Order Fabales.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is some isolates transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between hosts; not transmitted by seeds; not transmitted by pollen.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Hemiptera, family Aleyrodidae; Bemisia tabaci (Sida race). Virus is transmitted in a persistent manner; retained when the vector moults; does not replicate in the vector; not transmitted congenitally to the progeny of the vector.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Leguminosae-Papilionoideae, Malvaceae. The following species were susceptible to experimental virus infection: Cajanus cajan, Glycine max, Macroptilium lathyroides, Malvastrum coromandelianum, Pachyrhizus erosus, Phaseolus acutifolius, Phaseolus coccineus, Phaseolus longipedunculatus, Phaseolus lunatus, Phaseolus vulgaris, Vigna angularis, Vigna mungo.

Host:
Experimentally infected hosts mainly show symptoms of chlorosis.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Cyperaceae, Leguminosae-Papilionoideae. Species inoculated with virus that do not show signs of susceptibility: Glycine max, Rhynchosia minima.

Diagnostic Hosts

Diagnostic host species and symptoms:

Phaseolus vulgaris cv. Top Crop, Phaseolus lunatus — systemic vein chlorosis, golden mosaic. Diagnostic host: insusceptible host species Glycine max, Rhynchosia minima (distinguishes the virus from Rhynchosia mosaic).

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Phaseolus vulgaris cv. Top Crop.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Phaseolus vulgaris cvs Top Crop and Red Kidney (W) and Red Mexican 35 (L).

References to host data: Galvez and Castano (1976, J. Bird (unpublished).

Histopathology: Virus can be best detected in leaves, mesophyll, phloem and stems. Virions are found in the nucleus and nucleolus.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the nucleus. Nuclear inclusion bodies are crystals. Inclusions are hexagonally packed crystalline arrays or loose aggregates of virion-like particles in the nucleus. Other cellular changes include the occurrence of characteristic fibrillar rings in nuclei, after nucleoli undergo hypertrophy.

Geographical Distribution

The virus occurs in Brazil, the Dominican Republic, Guatemala, Jamaica, Puerto Rico, and Venezuela.

List of Strains and Isolates in the Species

Isolates from Venezuela and Puerto Rico differ in DNA hybridization analyses.

References

Bird, J., Perez, JE, Alconero, R., Vakili, N.G. and Melandez, P.L. (1972). J. agric. Univ. Puerto Rico 56: 64.

Galvez, G.E. and Castano, M. (1976). Turrialba 26: 205.

Goodman, RM (1977). Virology 83: 171.

Goodman, RM and Bird, J. (1978). CMI/AAB Descr. Pl. Viruses No. 192, 4 pp.

Goodman, RM, Bird, J. and Thongmeearkom, P. (1977). Phytopathology 67: 37.

Goodman, RM, Shock, T.L., Haber, S., Browning, K.S. and Bowers, G.R. (1980). Virology 106: 168.

Haber, S., Howarth, AJ. and Goodman, RM (1983). Virology 129: 517.

Howarth, AJ. and Vandemark, G.J. (1989). J. gen. Virol. 70: 2717.

Howarth, AJ., Caton, J., Bossert, M. and Goodman, RM (1985). Proc. Natl. Acad. Sci. U.S.A. 82: 3572.

Kim, K.S., Shock, T.L. and Goodman, RM (1978). Virology 89: 22.

The following generic references are cited in the most recent ICTV Report.

PubMed References. A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 70 by S. Haber and A.J. Howarth, 1983. Revised A.J. Howarth, 1990.
A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 192.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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