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Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

00.017.0.03.005. Pineapple mealybug wilt-associated virus 1

Cite this publication as: ICTVdB Management (2006). 00.017.0.03.005. Pineapple mealybug wilt-associated virus 1. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Hawaii; the United States of America.

Host of Isolate and Habitat Details
Source of isolate: Ananas comosus cv. Smooth Cayenne.

Natural host and symptoms
Ananas comosus — yellowing and flaccidity of young leaves, necrosis of leaf tips; plants wilt and may die.

Reference to Isolation Report
Carter (1933).

Classification

This is a description of a plant virus at the species level.

ICTVdB Virus Code: 00.017.0.03.005. Virus accession number: 17081026. Obsolete virus code: 00.017.0.81.026.; 17.0.1.T.2.02; superceded accession number: 17081026; 1701t202.
NCBI Taxon Identifier NCBI Taxonomy ID: 180903.

Name, Synonyms and Lineage

Synonym(s): pineapple wilt-associated virus (1 and/or 2 ?) (Gunasinghe et al., 1989; Ullman et al., 1989). ICTV approved acronym: PMWaV-1. Virus is an ICTV approved species of the genus Ampelovirus; family 00.017. Closteroviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. The capsid is filamentous, flexuous with a length of 1200-1500 nm and a width of 12 nm. Axial canal is indistinct.

Electron microscopic preparation and references: Virus preparation contains few virions. Reference for electron microscopic methods: Gunasinghe and German (1977). Extract tissue in 0.5M Tris, followed by PEG precipitation and differential centrifugation.

Physicochemical and Physical Properties

The density of virions is 1.2 g cm-3 in caesium sulphate. There are 1 sedimenting component(s) found in purified preparations.

Nucleic Acid

The genome is monopartite. Only one particle size of linear, positive-sense, single-stranded RNA is recovered. The complete genome is 7100 nucleotides long. Genome is sequenced, but only an estimate is available, complete sequence is 7100 nucleotides long.

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s).

Lipids

Lipids are not reported.

Diagnostics and Reference Collections

The best tests for diagnosis are detection of dsRNA can be used for diagnosis (Gunasinghe and German, 1986; 1988).

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

Severity and Occurrence of Disease

Host: Signs and symptoms persist, vary seasonally, and disappear soon after infection.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is not transmitted by mechanical inoculation.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Hemiptera, family Pseudococcidae; Pseudococcus brevipes. Virus is transmitted in a semi-persistent manner (possibly).

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Bromeliaceae. The following species were susceptible to experimental virus infection: Ananas comosus (cv. Smooth Cayenne).

Diagnostic Hosts

Diagnostic host species and symptoms:

Ananas comosus — young leaves become yellow and flacid with brown and necrotic tips. Plants wilt.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Ananas comosus.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Ananas comosus (W).

Histopathology: Virus can be best detected in leaves and phloem.

Geographical Distribution

The virus occurs in the United States of America (Hawaii).

Ecology, Epidemiology and Control

Studies reported by Gunasinghe and German (1986; 1988).

Comments

The virus can be eliminated from planting stock by hot water treatment of "crowns" for up to 120 minutes at 50°C.

References

Carter, W.C. (1933). Phytopathology 23: 207.

Gunasinghe, U.B. and German, T. (1986). Phytopathology 76: 1073.

Gunasinghe, U.B. and German, T. (1987). Phytopathology 77: 1776.

Gunasinghe, U.B. and German, T. (1988). Phytopathology 78: 1584.

Gunasinghe, U.B. and German, T.L. (1989). Phytopathology 79: 1337.

Ullman, D.E., German, T.L., Gunasinghe, U.B. and Ebesu, RH (1989). Phytopathology 79: 1341.

Ullman, D.E., German, T.L., McIntosh, C.E. and Williams, D.D.F. (1991). Plant Dis. 75: 859.

The following generic references are cited in the most recent ICTV Report.

PubMed References. A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 617 by A.A. Brunt and U.B. Gunasinghe, 1988. Updated 1991.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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