[Home] [ICTV Taxonomy - Index of Viruses] [Virus Descriptions] [Character List] [Picture Gallery]
[Tutorial] [Online Data Retrieval & Identification] [Virus Isolate Registration & Submission] [Search]
Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

00.010.0.03.007. Spring beauty latent virus

Cite this publication as: ICTVdB Management (2006). 00.010.0.03.007. Spring beauty latent virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Arkansas; the United States of America.

Host of Isolate and Habitat Details
Source of isolate: Claytonia virginica.

Natural host and symptoms
Claytonia virginica — symptomless.

Reference to Isolation Report
Valverde (1985).

Depositories and Collections

The isolate has been deposited at the American Type Culture Collection (ATCC) and is listed in the ATCC catalogue with the reference number [PV-396].

Classification

This is a description of a plant virus at the species level and isolate level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.010.0.03.007. Virus accession number: 10003007. Obsolete virus code: 10.0.3.0.007; superceded accession number: 10030007.
NCBI Taxon Identifier NCBI Taxonomy ID: 188141.

Name, Synonyms and Lineage

ICTV approved acronym: SBLV. Virus is an ICTV approved species of the genus 00.010.0.03. Bromovirus in the family 00.010. Bromoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 28 nm. Capsids appear round. The capsomer arrangement is not obvious. Virus preparations contain more than one particle component.




















Electron micrograph of Bromoviridae.

Electron microscopic preparation and references: Virus preparation contains many virions. Reference for electron microscopic methods: Valverde RA (1985) Phytopathology 75: 395.

Physicochemical and Physical Properties

There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 90 S20w. Under in vitro conditions virions are stable in acid environment of pH 7; stable in alkaline environment of pH 7.

Nucleic Acid

The genome is segmented; tripartite, segements are distribute among 3 particle types of different size; consists of three segments of to four segments of linear, positive-sense, single-stranded RNA. Minor species of genomic nucleic acid are found in addition to the full length genome; there are 1 minor species found; non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic mRNA; subgenomic RNA-4 derived from genomic RNA-3. The complete genome is 8363 nucleotides long. Sequence can be accessed from EBI-EMBL, or GenBank, or Rothamsted; the RNA-1 is fully sequenced, complete sequence is 3252 nucleotides long and encodes protein 1a. Sequence has the accession number [AB080598]. RNA-2 is fully sequenced, complete sequence is 2898 nucleotides long and encodes protein 2a has the accession number
[AB080599]. RNA-3 is fully sequenced. Complete sequence is 2213 nucleotides long and encodes protein 3a, which is not essential for viral RNA replication, but is required for viral movement and the coat protein, which is translated from the subgenomic RNA-4; has the accession number
[AB080600]. RNA-4 is a subgenomic mRNA derived from RNA-3 negative strand template. The genome has a base ratio of 23.8 % guanine; 27.7 % adenine; 18.3 % cytosine; 30.2 % uracil. The genome has a guanine + cytosine content of 40.1 %. The multipartite genome is divided among more than one type of particle and the segments are distributed between 3 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B). The medium sized particles contain each one molecule of RNA-2 (sedimenting component M). The smallest particles contain one molecule each of RNA-3 and RNA-4 (sedimenting component T). Reference to nucleotide sequence Valverde (1985).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein is involved in viral encapsidation and involved in protein movement.

Reference to method of preparation: Valverde (1985).

Lipids

Lipids are absent.

Genome Organization and Replication

By itself, genomic nucleic acid is infectious.

Transcription: Sub-genomic RNA is present in infected cells; encoding the coat protein. The genome expression is based on RNA production which can be analyzed by the dsRNA patterns found in the infected tissues. Usually there are 5 virus specified dsRNA species found in infected cells. Size of largest virus specified dsRNA 2.2 kbp. 2nd largest 2 kbp. 3rd largest 1.4 kbp. 4th largest 1.2 kbp. 5th largest 0.5 kbp.

Antigenicity

The virus is serologically related to broad bean mottle, brome mosaic, cowpea chlorotic mottle and melandrium yellow fleck viruses.

Diagnostics and Reference Collections

The best tests for diagnosis are serology and double diffusion tests.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass CARYOPHYLLIDAE; Order Caryophyllales.

Severity and Occurrence of Disease

Host: Infection is not apparent.

Transmission and Vector Relationships

Possibly on lawn mowers and tractors in lawns and public areas. Virus is transmitted by mechanical inoculation; transmitted by grafting; possibly transmitted by contact between hosts; not transmitted by seeds.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Amaranthaceae, Apocynaceae, Chenopodiaceae, Commelinaceae, Cruciferae, Cucurbitaceae, Leguminosae-Papilionoideae, Portulacaceae, Solanaceae. The following species were susceptible to experimental virus infection: Arabidopsis thaliana, Catharanthus roseus, Chenopodium album, Chenopodium amaranticolor, Chenopodium quinoa, Claytonia virginica, Commelina diffusa, Cucumis sativus, Cucurbita pepo, Datura stramonium, Glycine max, Gomphrena globosa, Nicotiana clevelandii, Nicotiana debneyi, Nicotiana glutinosa, Nicotiana megalosiphon, Phaseolus vulgaris, Pisum sativum, Vicia faba, Vigna radiata, Vigna unguiculata, Vigna unguiculata ssp. unguiculata (cv. Monarch).

Host:
Experimentally infected hosts mainly show symptoms of necrotic local lesions, systemic mottle and necrosis.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Compositae, Cucurbitaceae, Gramineae, or Portulacaceae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Avena sativa, Capsicum annuum, Cucumis melo, Lycopersicon esculentum, Nicotiana tabacum, Portulaca oleracea, Triticum aestivum, Zea mays, Zinnia elegans.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium quinoa, C. album, C. amaranticolor, Cucurbita pepo cv. Small Sugar, Datura stramonium, Glycine max cvs Lee and Bragg, Phaseolus vulgaris cvs Pinto, Bountiful, Black Valentine, Vigna radiata, V. unguiculata ssp. unguiculata cv. Monarch — necrotic local lesions.

Cucumis sativus cv. Model — chlorotic local lesions.

Commelina diffusa — systemic mottle.

Gomphrena globosa — necrotic local lesions, systemic mottle and necrosis.

Nicotiana megalosiphon, N. clevelandii — necrotic local lesions, systemic necrosis.

Nicotiana debneyi — necrotic local lesions, systemic mottle.

Pisum sativum cv. little Marvel — systemic mottle and necrosis.

Vicia faba — systemic necrosis.

Catharanthus roseus — systemic necrosis and mottle. Diagnostic host: insusceptible host species Avena sativa, Capsicum annuum, Lycopersicon esculentum, Nicotiana tabacum cv. Havana 38, Zea mays.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Commelina diffusa, Gomphrena globosa, Nicotiana clevelandii, Pisum sativum, Catharanthus roseus.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium quinoa (L), Datura stramonium (L), Gomphrena globosa (L and W), Pisum sativum (W), Vicia faba (W).

References to host data: Valverde (1985).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm.

Geographical Distribution

The virus occurs in the United States of America.

References

Valverde, R.A. (1985). Phytopathology 75: 395.

Valverde, R.A. and Glascock, C.B. (1991). Phytopathology 81: 401.

The following generic references are cited in the most recent ICTV Report.

PubMed References. A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 751 by R.A. Valverde, 1991.



Limit search to: Title & Body Title Document Path
Show Reverse Sort

DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

ICTVdB and DELTA related References

Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
Copyright © 2002    International Committee on Taxonomy of Viruses.    All rights reserved.


Additional access points to virus species lists, descriptions and images on the web:

Species catalogue                     iSpecies.org - a species search engine           a species search engine

Google Analytics      Google Analytics: activity view