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00.010.0.03.004. Cassia yellow blotch virus

Cite this publication as: ICTVdB Management (2006). 00.010.0.03.004. Cassia yellow blotch virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Mitchell, Queensland; Australia.

Host of Isolate and Habitat Details
Source of isolate: Cassia pleurocarpa.

Natural host and symptoms
Cassia pleurocarpa — yellow leaf blotches.

Reference to Isolation Report
Dale et al. (1984).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.010.0.03.004. Virus accession number: 10003004. Obsolete virus code: 10.0.3.0.004; superceded accession number: 10030004.
NCBI Taxon Identifier NCBI Taxonomy ID: 300879.

Name, Synonyms and Lineage

ICTV approved acronym: CYBV. Virus is an ICTV approved species of the genus 00.010.0.03. Bromovirus in the family 00.010. Bromoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 26 nm. Capsids appear round. The capsomer arrangement is not obvious. Virus preparations contain more than one particle component.




















Electron micrograph of Bromoviridae.

Electron microscopic preparation and references: Virus preparation contains many virions. Best with UA or AM. Reference for electron microscopic methods: Bancroft et al. (1967, Dale et al. (1984).

Physicochemical and Physical Properties

There are 1 sedimenting component(s) found in purified preparations (at pH 7). The sedimentation coefficient is 85 S20w. Isoelectric point pH is 3.6. The infectivity is retained when deproteinized with phenol or detergent.

Nucleic Acid

The genome is segmented; tripartite, segements are distribute among 3 particle types of different size; consists of three segments of to four segments of linear, positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA, that is mRNA derived from genomic RNA-3. The complete genome is 8700 nucleotides long, is sequenced, complete sequence is about 3400 nucleotides long. RNA-2 is sequenced, but only an estimate is available, complete sequence is 3000 nucleotides long. RNA-3 is sequenced, but only an estimate is given, complete sequence is 2300 nucleotides long. The genome has a base ratio of 24.4 % guanine; 24.9 % adenine; 22.2 % cytosine; 28.5 % uracil. The multipartite genome is divided among more than one type of particle and the segments are distributed between 3 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B). The medium sized particles contain each one molecule of RNA-2 (sedimenting component M). The smallest particles contain one molecule each of RNA-3 and RNA-4 (sedimenting component T). Reference to nucleotide sequence Dale et al. (1984).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein is involved in viral encapsidation and involved in protein movement.

Reference to amino acid sequence or composition Dale et al. (1984).

Lipids

Lipids are absent.

Genome Organization and Replication

By itself, genomic nucleic acid is infectious.

Transcription: Sub-genomic RNA is present in infected cells; encoding the coat protein.

Antigenicity

The virus is serologically related to broad bean mottle, brome mosaic and cowpea chlorotic mottle viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ROSIDAE; Order Fabales.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by mechanical inoculation.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Gramineae, Leguminosae-Caesalpinioideae, Leguminosae-Papilionoideae, Solanaceae. The following species were susceptible to experimental virus infection: Cassia hirsuta, Cassia occidentalis, Cassia pleurocarpa, Cassia tora, Chenopodium amaranticolor, Chenopodium quinoa, Cicer arietinum, Cucumis sativus, Desmodium tortuosum, Glycine max, Gomphrena globosa, Lathyrus odoratus, Lupinus angustifolius, Macroptilium lathyroides, Nicotiana clevelandii, Phaseolus vulgaris, Trifolium incarnatum, Zea mays.

Host:
Experimentally infected hosts mainly show symptoms of chlorotic local lesions, mottle, tip necrosis.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Compositae, Gramineae, or Leguminosae-Papilionoideae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Arachis hypogaea, Avena sativa, Cajanus cajan, Cicer arietinum, Clitoria ternatea, Datura stramonium, Helianthus annuus, Hordeum vulgare, Medicago sativa, Pisum sativum, Sorghum bicolor, Trifolium pratense, Triticum aestivum, Vigna radiata, Vigna unguiculata ssp. sesquipedalis .

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium amaranticolor, C. quinoa — chlorotic local lesions; not systemic.

Cassia hirsuta, C. occidentalis, Trifolium incarnatum — systemic chlorotic mottle.

Cicer arietinum — tip necrosis.

Nicotiana clevelandii — systemic green mottle. Diagnostic host: insusceptible host species Arachis hypogaea, Pisum sativum, Vigna radiata.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Nicotiana clevelandii.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium amaranticolor (L).

References to host data: Dale et al. (1984).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm and nucleus.

Cytopathology: Inclusions are not present in infected cells.

Geographical Distribution

The virus occurs in Australia.

References

Bancroft, J.B., Hills, G.J. and Markham, R. (1967). Virology 31: 354.

Dale, J.L. (1988). CMI/AAB Descr. Pl. Viruses No. 334, 3 pp.

Dale, J.L., Gibbs, AJ. and Behncken, GM (1984). J. gen. Virol. 65: 281.

The following generic references are cited in the most recent ICTV Report.

PubMed References. A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 182 by J.L. Dale, 1984. Revised 1987.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 334.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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