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00.010.0.03.002. Broad bean mottle virus

Cite this publication as: ICTVdB Management (2006). 00.010.0.03.002. Broad bean mottle virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: the United Kingdom.

Host of Isolate and Habitat Details
Source of isolate: Vicia faba.

Natural host and symptoms
Vicia faba — leaf mottling and malformation.

Reference to Isolation Report
Bawden et al. (1951).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.010.0.03.002. Virus accession number: 10003002. Obsolete virus code: 10.0.3.0.002; superceded accession number: 10030002.
NCBI Taxon Identifier NCBI Taxonomy ID: 12301.

Name, Synonyms and Lineage

ICTV approved acronym: BBMV. Virus is an ICTV approved species of the genus 00.010.0.03. Bromovirus in the family 00.010. Bromoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 26 nm. Capsids appear round. The capsomer arrangement is not obvious. Virus preparations contain more than one particle component.




















Electron micrograph of Bromoviridae.

Electron microscopic preparation and references: Virus preparation contains many virions. Reference for electron microscopic methods: Lane (1974).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.36 g cm-3 (pH 6 or lower). There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 85 S20w (pH 7). Isoelectric point pH is 5.6 (and 0.1 ionic strength). A260/A280 ratio is 5.4. The thermal inactivation point (TIP) is at 95°C. The longevity in vitro (LIV) is 20 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 3. The infectivity is not changed by treatment with ether; retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 21-23% of the virion by weight. The genome is segmented; tripartite, segements are distribute among 3 particle types of different size; consists of three segments of to four segments of linear, positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA. The complete genome is 8250 nucleotides long, is fully sequenced, complete sequence is 3158 nucleotides long. Sequence has the accession number

[K01776] Em(40)_vi:BRRNA1 Gb(84)_vi:BBMRNA1 Broad bean mottle virus RNA 1, 3' end. 6/94 200bp.
[K01777] Em(40)_vi:BRRNA2 Gb(84)_vi:BBMRNA2 Broad bean mottle virus RNA 2, 3' end. 6/94 214bp.
[K01778] Em(40)_vi:BRRNA3 Gb(84)_vi:BBMRNA3 Broad bean mottle virus RNA 3/RNA 4, 3' end. 6/94 266bp.
[M60291] Em(40)_vi:BBM3ACT Gb(84)_vi:BBM3ACT Broad bean mottle virus 3a and coat proteins, complete cds. 4/94 2,293bp.
[M64713] Em(40)_vi:BBMRNA2Q Gb(84)_vi:BBMRNA2Q Broad bean mottle virus RNA 2, complete cds. 5/91 2,799bp.
[M65138] Em(40)_vi:BBMIAP Gb(84)_vi:BBMIAP Broad bean mottle virus (BBMV) 1a protein gene, complete cds. 8/92 3,158bp.
[U24495] Em(43)_vi:Bb24495 Gb(89)_vi:Bbu24495 Broad bean mottle virus Type strain 2a protein gene, complete cds. 5/95 2,834bp.
[U24496] Em(43)_vi:Bb24496 Gb(89)_vi:Bbu24496 Broad bean mottle virus Mo strain 2a protein gene, complete cds. 5/95 2,917bp.

RNA-2 is sequenced, complete sequence is about 2799 nucleotides long. RNA-3 is sequenced, but only an estimate is given, complete sequence is 2293 nucleotides long. The genome has a base ratio of 25 % guanine; 27 % adenine; 19 % cytosine; 29 % uracil. The 5'-end of the genome has a methylated nucleotide cap. The 3'-terminus has a tRNA-like structure that can be aminoacylated with tyrosine. The multipartite genome is divided among more than one type of particle and the segments are distributed between 3 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B). The medium sized particles contain each one molecule of RNA-2 (sedimenting component M). The smallest particles contain one molecule each of RNA-3 and RNA-4 (sedimenting component T).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 77-79% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein is involved in viral encapsidation and involved in protein movement.

Reference to method of preparation: Gibbs (1972): 1M NaCl and reducing agent.

Lipids

Lipids are absent.

Polyamines

Other compounds that have been detected in the virus particles are spermidine.

Transcription: Sub-genomic RNA is present in infected cells.

Translation: Coat protein mRNA is translated in the cytoplasm (cycloheximide sensitive).

Antigenicity

The virus is serologically related to brome mosaic and cowpea chlorotic mottle viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ROSIDAE; Order Fabales.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seeds.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Coleoptera; Acalymma trivittata, Colaspis flavida, Diabrotica undecimpunctata, Sitona lineata.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Amaranthaceae, Chenopodiaceae, Leguminosae-Papilionoideae, Solanaceae. The following species were susceptible to experimental virus infection: Chenopodium album, Chenopodium amaranticolor, Chenopodium hybridum, Chenopodium quinoa, Cyamopsis tetragonoloba, Glycine max, Gomphrena globosa, Lathyrus odoratus, Lupinus albus, Medicago sativa, Melilotus albus, Nicotiana clevelandii, Phaseolus vulgaris, Pisum sativum, Trifolium hybridum, Trifolium incarnatum, Trifolium pratense, Trifolium repens, Trifolium subterraneum, Vicia faba, Vicia villosa.

Host:
Experimentally infected hosts mainly show symptoms of local lesions, mosaics, mottles.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Chenopodiaceae, Cucurbitaceae, or Leguminosae-Papilionoideae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Cucumis sativus, Datura stramonium, Lablab purpureus, Lycopersicon pimpinellifolium, Nicotiana glutinosa, Nicotiana tabacum, Spinacia oleracea, Vicia sativa, Vigna radiata, Vigna unguiculata.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium amaranticolor — chlorotic local lesions; not systemic.

Cyamopsis tetragonoloba — necrotic local lesions or streaks.

Phaseolus vulgaris cv. Canadian Wonder — chlorotic local lesions; systemic mottle and vein yellowing or netting.

Pisum sativum — lethal systemic wilt.

Vicia faba — systemic blotchy mottle.

Nicotiana clevelandii — mild mottle. Diagnostic host: insusceptible host species Nicotiana tabacum.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Vicia faba, Nicotiana clevelandii.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Cyamopsis tetragonoloba (L).

References to host data: Thornberry (1966, Lane (1974; 1981).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are amorphous X-bodies. Inclusions contain mature virions.

Geographical Distribution

The virus occurs in Portugal, Sudan, and the United Kingdom.

References

Bawden, F.C., Chaudhuri, R.P. and Kassanis, B. (1951). Ann. appl. Biol. 38: 774.

Dzianott, AM. and Bujarski, J.J. (1991). Virology 185: 553.

Gibbs, AJ. (1972). CMI/AAB Descr. Pl. Viruses No. 101, 4 pp.

Lane, LC (1974). Adv. Virus Res. 19: 151.

Lane, LC (1979). CMI/AAB Descr. Pl. Viruses No. 215, 4 pp.

Lane, LC (1981). In: Handbook of Pl. Virus Infections and Comparative Diagnosis, p.333; Ed. E. Kurstak. Elsevier/North Holland Biomedical Press, Amsterdam.

Thornberry, HH (1966). In: Index of Plant Virus Diseases. U.S. Dep. Agric. Hdbk No. 307, p.235.

The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 109 by A.J. Gibbs, 1981, Revised 1985.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 101.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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