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00.010.0.02.012. Lilac ring mottle virus

Cite this publication as: ICTVdB Management (2006). 00.010.0.02.012. Lilac ring mottle virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: the Netherlands.

Host of Isolate and Habitat Details
Source of isolate: Syringa vulgaris.

Natural host and symptoms
Syringa vulgaris — faint rings and line patterns.

Reference to Isolation Report
Van der Meer (1976).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.010.0.02.012. Virus accession number: 10002012. Obsolete virus code: 10.0.2.07.01; superceded accession number: 10020701.
NCBI Taxon Identifier NCBI Taxonomy ID: 37125.

Name, Synonyms and Lineage

ICTV approved acronym: LiRMoV. Acronym(s): LRMV. Virus is an ICTV approved species. Virus is of the genus 00.010.0.02. Ilarvirus in the family 00.010. Bromoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid is round to elongated with icosahedral symmetry. The capsid is isometric and has a diameter of 27 nm. Capsids appear round. The capsomer arrangement is not obvious. Virus preparations contain more than one particle component.
























Electron micrograph of Bromoviridae.

Electron microscopic preparation and references: Virus preparation contains few virions. Fix virions with 2% glutaraldehyde. Reference for electron microscopic methods: Van der Meer et al. (1976).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.345 g cm-3. The density of virions is 1.265 in Cs2SO4. There are 2 sedimenting component(s) found in purified preparations. The sedimentation coefficient is of the fastest (B) component 98 S20w; of the other(s) are 83 S20w (M). A260/A280 ratio is 1.43-1.46. The thermal inactivation point (TIP) is at 63-65°C. The longevity in vitro (LIV) is 0.2 days (at 20°C). Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 4 (in sap of Chenopodium quinoa). The infectivity is not changed by treatment with ether; retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 13-14% of the virion by weight. The genome is segmented; tripartite, segements are distribute among 3 particle types of different size; consists of four segments of linear positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA, that is mRNA derived from genomic RNA-3. The complete genome is 9042 nucleotides long, is fully sequenced, complete sequence is 3324 nucleotides long. RNA-1 has the accession number [U17391] Em(43)_vi:Lr17391 Gb(89)_vi:Lru17391 Lilac ring mottle virus RNA 3 movement protein and coat protein genes, complete cds. 5/95.

RNA-2 is sequenced, complete sequence is about 3183 nucleotides long. RNA-3 is sequenced, but only an estimate is given, complete sequence is 2535 nucleotides long. RNA-4 is subgenomic has been sequenced, but only an estimate is provided, complete sequence is 1042 nucleotides long. Subgenomic RNA-4 is an mRNA derived from RNA-3 negative strand template. The multipartite genome segments are distributed between 3 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B). The medium sized particles contain each one molecule of RNA-2 (sedimenting component M). The smallest particles contain one molecule each of RNA-3 and RNA-4 (sedimenting component T). Reference to nucleotide sequence Huttinga and Mosch (197.

Reference to nucleotide sequence in PubMed: nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 86-87% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein is involved in viral encapsidation and involved in protein movement.

Reference to method of preparation: Huttinga and Mosch (1976).

Lipids

Lipids are absent.

Genome Organization and Replication

By itself, genomic nucleic acid is not infectious; a successful infection by the virus requires the coat protein specifically associated with RNA 3'-terminal sequences for replicase recognition.

Transcription: Sub-genomic RNA is present in infected cells.

Antigenicity

The virus does not show serological relationships to apple mosaic, elm mottle, Prunus necrotic ringspot, spinach latent and tobacco streak viruses.

Serological tests quickly distinguish lilac ring mottle virus from related viruses.

Diagnostics and Reference Collections

The best tests for diagnosis are ten other sap transmissible viruses are known to infect lilac (Van der Meer, 1980, 1981; Waterworth, 1972), thus identification of lilac ring mottle virus by testing in standard indicator plants, like Chenopodium and Nicotiana species, is unreliable and serological tests are required.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ASTERIDAE; Order Scrophulariales.

Severity and Occurrence of Disease

Host: Signs and symptoms vary cyclically over a few weeks and vary seasonally (infected plants show symptoms in spring; cvs Mme Florent Stepman and Mme Lemoine show clear symptoms).

Transmission and Vector Relationships

Virus is transmitted by mechanical inoculation; transmitted by grafting; transmitted by contact between hosts; transmitted by seeds (in Chenopodium quinoa, C. amaranticolor, but not in lilac (Van der Meer, 1976)).

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Amaranthaceae, Apocynaceae, Chenopodiaceae, Leguminosae-Papilionoideae, Oleaceae, Solanaceae. The following species were susceptible to experimental virus infection: Catharanthus roseus, Celosia argentea, Celosia plumosa, Chenopodium album, Chenopodium amaranticolor, Chenopodium foetidum, Chenopodium quinoa, Gomphrena globosa, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana megalosiphon, Nicotiana rustica, Nicotiana tabacum, Phaseolus vulgaris, Syringa vulgaris, Vigna unguiculata.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Cucurbitaceae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Amaranthus caudatus, Capsicum annuum, Cucumis sativus, Lycopersicon esculentum.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium quinoa — systemic epinasty and tip necrosis.

Celosia plumosa — chlorotic flecks, then systemic chlorosis.

Nicotiana megalosiphon — necrotic local lesions, then systemic mottling.

Nicotiana tabacum — transient wilting of the first leaves to be systemically infected. Diagnostic host: insusceptible host species Cucumis sativus, Amaranthus caudatus, Capsicum annuum, Lycopersicon esculentum.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Chenopodium quinoa.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium quinoa (W).

References to host data: Van der Meer (1976).

Geographical Distribution

The virus is found, but with no evidence of proliferation, in Canada, the Netherlands and the U.K.

Ecology, Epidemiology and Control

Studies reported by Van der Meer (1976, Van der Meer et al. (1976).

References

Bos, L., Huttinga, H. and Maat, D.Z. (1980). Neth. J. Pl. Path. 86: 79.

Fulton, R.W. (1983). CMI/AAB Descr. Pl. Viruses No. 275, 3 pp.

Huttinga, H. and Mosch, W.H.M. (1976). Acta Hort. 59: 113.

Huttinga, H., Maat, D.Z. and van der Meer, F.A. (1976). Acta Bot. neerl. 25: 251.

Van der Meer, F.A. (1976). Acta Hort. 59: 105.

Van der Meer, F.A. (1980). Acta Hort. 110: 211.

Van der Meer, F.A. and Huttinga, H. (1979). CMI/AAB Descr. Pl. Viruses No. 201, 4 pp.

Van der Meer, F.A., Huttinga, H. and Maat, D.Z. (1976). Neth. J. Pl. Path. 82: 67.

Waterworth, H.E. (1972). Pl. Dis. Reptr 56: 923.

The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 445 by F.A. van der Meer, 1987.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 201.



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ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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