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00.010.0.02.006. Citrus leaf rugose virus

Cite this publication as: ICTVdB Management (2006). 00.010.0.02.006. Citrus leaf rugose virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: the United States of America.

Host of Isolate and Habitat Details
Source of isolate: citrus limon.

Natural host and symptoms
citrus aurantifolia, C. limon, C. paradisi — flecking of young leaves and malformation of old leaves (particularly of Eureka lemons), fruit small and lumpy.

Phaseolus vulgaris cv. Red Kidney — necrotic local lesions, no systemic infection.

Reference to Isolation Report
Fawcett (1936).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.010.0.02.006. Virus accession number: 10002006. Obsolete virus code: 10.0.2.02.03; superceded accession number: 10020203.
NCBI Taxon Identifier NCBI Taxonomy ID: 37126.

Name, Synonyms and Lineage

Synonym(s): citrus psorosis virus complex, citrus crinkly leaf ilarvirus. ICTV approved acronym: CiLRV. Virus is an ICTV approved species of the genus 00.010.0.02. Ilarvirus in the family 00.010. Bromoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid is round to elongated with icosahedral symmetry. The capsid is quasi-isometric and has a diameter of 25-32 nm (Yot-Dauthy and Bov‚, 1968). Virus preparations contain more than one particle component. Virions have isometric or irregularly shaped capsid.
























Electron micrograph of an Ilarvirus, Bromoviridae.

Electron microscopic preparation and references: Reference for electron microscopic methods: Yot-Dauthy and Bov‚ (1968, Garnsey (1975).

Physicochemical and Physical Properties

There are 4 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 105 S20w; of the other(s) are 98 S20w (also 89 and 79). A260/A280 ratio is 1.4-1.46 (mixed components).

Nucleic Acid

The genome is segmented; tripartite, segements are distribute among 3 particle types of different size; consists of four segments of linear positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA, that is mRNA derived from genomic RNA-3. The complete genome is 7888 nucleotides long. Sequence can be accessed from EBI-EMBL and GenBank. The RNA-1 is fully sequenced. Complete sequence is 3099 nucleotides long. Sequence has the accession number
[U17390] Em(43)_vi:Cl17390 Gb(89)_vi:Clu17390 citrus leaf rugose virus RNA 3 movement protein and coat protein genes, complete cds. 4/95 2,
[U17726] Em(43)_vi:Cl17726 Gb(89)_vi:Clu17726 citrus leaf rugose virus RNA-2, polymerase gene, complete cds. 2/95 2,990bp.
[U23715] Em(44)n:Cl23715 Gb(90)n:Clu23715 citrus leaf rugose ilarvirus putative viral replicase gene, complete cds. 8/95 3,404bp.

RNA-2 is sequenced, complete sequence is about 2817 nucleotides long. RNA-3 is sequenced, but only an estimate is given, complete sequence is 1972 nucleotides long. Subgenomic RNA-4 is an mRNA derived from RNA-3 negative strand template. The multipartite genome is divided among more than one type of particle and the segments are distributed between 3 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B). The medium sized particles contain each one molecule of RNA-2 (sedimenting component M). The smallest particles contain one molecule each of RNA-3 and RNA-4 (sedimenting component T).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein is involved in viral encapsidation and involved in protein movement.

Lipids

Lipids are absent.

Transcription: Sub-genomic RNA is present in infected cells; encoding the coat protein.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ROSIDAE; Order Sapindales Family Rutaceae.

Severity and Occurrence of Disease

Host: Signs and symptoms persist, or vary seasonally.

Transmission and Vector Relationships

Virus is not transmitted by a vector. Virus is transmitted by mechanical inoculation; transmitted by grafting; possibly transmitted by seeds.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Apocynaceae, Leguminosae-Papilionoideae, Rutaceae. The following species were susceptible to experimental virus infection: Catharanthus roseus, Citrus aurantifolia, Citrus limon, Citrus paradisi, Citrus reticulata, Citrus sinensis, Crotalaria spectabilis, Phaseolus vulgaris (cv. Red Kidney), Vigna unguiculata.

Diagnostic Hosts

Diagnostic host species and symptoms:

citrus limon Eureka lemon — asteroid local lesions.

citrus sinensis, C. limon, C. reticulata — crinkling and flecking.

Vigna unguiculata — vein clearing, malformation.

Crotalaria spectabilis — systemic chlorotic veinbanding.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are any citrus species.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Vigna unguiculata cv. 'Blackeye' No. 5. (L).

Geographical Distribution

The virus occurs in the United States of America (California). The virus is found, but with no evidence of proliferation, in Australia.

References

Attathom, S., Weathers, L.G. and Greer, F.C. (1975). Proc. Am. Phytopath. Soc. 2: 111.

Fawcett, H.S. (1936). In: citrus Diseases and their Control. McGraw Hill, New York, London, 656 pp.

Garnsey, SM (1975). Phytopathology 65: 50.

Gonsalves, D. and Garnsey, SM (1974). Virology 61: 343.

Gonsalves, D. and Garnsey, SM (1975a). Virology 64: 23.

Gonsalves, D. and Garnsey, SM (1975b). Virology 67: 311.

Gonsalves, D. and Garnsey, SM (1975c). Virology 67: 319.

Gonsalves, D. and Garnsey, SM (1976). CMI/AAB Descr. Pl. Viruses No. 164, 4pp.

Yot Dauthy, D. and Bov‚, J.M. (1968). Proc. 4th Conf. Int. Org. citrus Virol., p. 255.

The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 218 by P. Barkley, 1987. Checked by R.F. Lee, 1989.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 164.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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