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00.010.0.02.005. Blueberry shock virus

Cite this publication as: ICTVdB Management (2006). 00.010.0.02.005. Blueberry shock virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Washington State; the United States of America.

Host of Isolate and Habitat Details
Source of isolate: Vaccinium corymbosum.

Natural host and symptoms
Vaccinium corymbosum — leaf and flower necrosis during bloom, plants show symptoms for 1-4 years, then recover and remain symptomless and produce good quality fruit.

Reference to Isolation Report
MacDonald et al. (1991).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.010.0.02.005. Virus accession number: 10002005. Obsolete virus code: 10.0.2.02.02; superceded accession number: 10020202.

Name, Synonyms and Lineage

Synonym(s): Blueberry necrotic shock virus. ICTV approved acronym: BlShV. Virus is an ICTV approved species of the genus Ilarvirus; family 00.010. Bromoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid is round to elongated with icosahedral symmetry. The capsid is isometric to quasi-isometric and has a diameter of 27 nm. Capsids appear hexagonal in outline. The capsomer arrangement is not obvious. Virus preparations contain more than one particle component.
























Electron micrograph of Bromoviridae.

Electron microscopic preparation and references: Virus preparation contains few virions. Reference for electron microscopic methods: MacDonald et al. (1991).

Physicochemical and Physical Properties

There are 2 sedimenting component(s) found in purified preparations. A260/A280 ratio is 1.46-1.6 (uncorrected for light scattering).

Nucleic Acid

The genome is segmented; tripartite, segements are distribute among 3 particle types of different size; consists of four segments of linear positive-sense, single-stranded RNA. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA.

Comment by the editor: Although the genome hasn't been sequenced the author of this description provided the following data.
The complete genome is estimated to be 7717 nucleotides long. The complete sequence of RNA-1 is estimated to be 2901 nt; RNA-2 about 2366 nt; RNA-3 about 1605 nucleotides long. RNA-4 is subgenomic is estimated to be 845 nucleotides long. Subgenomic RNA-4 is an mRNA derived from RNA-3 negative strand template.

The multipartite genome segments are distributed between 3 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B). The medium sized particles contain each one molecule of RNA-2 (sedimenting component M). The smallest particles contain one molecule each of RNA-3 and RNA-4 (sedimenting component T). Reference to nucleotide sequence proteinase K digest of coat protein.

Proteins

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein is involved in viral encapsidation and involved in protein movement.

Reference to method of preparation: MacDonald et al. (1991).

Lipids

Lipids are absent.

Antigenicity

The virus is serologically related to apple mosaic and prunus necrotic ringspot viruses. The virus does not show serological relationships to asparagus 2, citrus leaf rugose, citrus variegation, elm mosaic, elm mottle, Humulus japonicus, prune dwarf, spinach latent, tobacco streak and Tulare apple mosaic viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass DILLENIIDAE; Order Ericales.

General Symptoms in Plants Symptoms leaf and flower necrosis.

Severity and Occurrence of Disease

Host: Signs and symptoms vary seasonally.

Transmission and Vector Relationships

Virus spreads quickly in a crop. Removal of infected plants does not slow the spread of the virus significantly. Virus is transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between hosts.

Vector Transmission: Virus is not transmitted by Fimbriaphis fimbriata, Myzus persicae.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Ericaceae, Solanaceae. The following species were susceptible to experimental virus infection: Nicotiana benthamiana, Nicotiana clevelandii, Nicotiana sylvestris, Nicotiana tabacum, Vaccinium corymbosum.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Chenopodiaceae, Cruciferae, Cucurbitaceae, Leguminosae-Papilionoideae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Brassica juncea, Chenopodium amaranticolor, Chenopodium quinoa, Cucumis sativus, Gomphrena globosa, Lycopersicon esculentum, Petunia x hybrida, Phaseolus vulgaris.

Diagnostic Hosts

Diagnostic host species and symptoms:

Nicotiana benthamiana, N. clevelandii, N. sylvestris, N. tabacum cvs Samsun and Havana-425 — necrotic local lesions; systemic necrosis.

Vaccinium corymbosum — necrosis of leaves and flowers.

Diagnostic host: insusceptible host species Chenopodium quinoa, Cucumis sativus, Gomphrena globosa, Petunia x hybrida, Phaseolus vulgaris.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Nicotiana benthamiana, N. clevelandii, Vaccinium corymbosum.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Nicotiana clevelandii (W).

References to host data: MacDonald et al. (1991).

Histopathology: Virus can be best detected in leaves, roots and mesophyll. Virions are found in the cytoplasm.

Geographical Distribution

The virus occurs in the United States of America (Oregon and Washington State).

References

MacDonald, S.G., Martin, R.R. and Bristow, P.R. (1989). Acta Hort. 241: 295.

MacDonald, S.G., Martin, R.R. and Bristow, P.R. (1991). Phytopathology 81: 210.

The following generic references are cited in the most recent ICTV Report.
A description of this taxon can also be found on the web at VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description; VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 102 by R.R. Martin, 1991.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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