Launch guide window

Launch NCBI Home Page

Goals

• Find BioAssays that target estrogen receptors
• Find a population of analogs for estrogen, tamoxifen and testosterone in PubChem Compounds
• Using the assays, display estrogen receptor binding activity for these compounds

Steps

1. Find confirmatory BioAssays that target estrogen receptors
   
   • On the NCBI home page, select PubChem BioAssay from the Database menu, enter "estrogen receptor"[proteintargetname] in the search box and click Go.
   • Select PubChem BioActivity Summary from the Display menu.
   • Select the Confirmatory assays from the list (check the boxes to their left).
   • Click the Data Table tab at the top of the page.

2. Retrieve PubChem Compound records for estrogen, tamoxifen and testosterone
   
   • Open a new tab or window in your web browser, and navigate to the NCBI home page.
   • Select PubChem Compound from the Database menu, enter estrogen OR tamoxifen OR testosterone in the search box and click Go.
   • Click the Limits tab, and select Synonym from the All Fields menu. Click Go.

3. Retrieve assay data for these compounds
   
   • Go back to your browser tab/window that contains the assay data table you generated before.
   • Click the Select tab from the second row of tabs.
   • Scroll down to the Other Filters section, and click the + next to Compound Filter.
   • Select your estrogen OR tamoxifen OR testosterone query from the PubChem Compound Entrez History menu.
   • Click the Show button at the bottom of the page.

4. Analyze the results
   
   • Which compounds were tested? (Click on their CID/SID to find out.) Which ones are active? Inactive?
   • In which assay(s) were these compounds studied?
   • To download the data table, click on the Download button and follow the directions.

Revised February 13, 2008