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The Easiest Route to Metazoan Heat Shock

(How can I find if my bacterial protein has significant similarity to any metazoan proteins?)

  Sample User Question
Analysis/Comments
Flow Chart
Step By Step Guide
Additional Notes
 

Sample User Question back to top

I am interested in studying the evolution of a protein sequence (Chaperone protein dnaK (Heat shock protein 70) Accession Number NP_212652). I would like to find the closest matches in higher animals. What is the easiest way to do this?

Analysis/Comments back to top

You could run a BLAST search for the protein, then look at the results and choose the metazoan representatives. An easier method is to use BLink and eliminate all the taxonomic groups except the one of interest.

Flow Chart back to top

  1. NP_212652 Entrez Protein Record - Look at record and access BLink from here

  2. NP_212652 BLink Record

Step By Step Guide back to top

Entrez Protein

  1. Find Entrez Protein entry for appropriate protein
  2. Examine the record and be sure it is the appropriate entry
  3. Click the BLink Link
BLink

  1. Click the underlined name of each taxonomic group that you would like to eliminate from the list or click in the colored box preceeding the group that you would like to select.
  2. BLink will remove all groups but the one chosen.

Additional Notes back to top

Elimination of all taxonomic groups but one is a new feature of BLink. The selective elimination of multiple groups

Similarity Searching/BLink User Question 1 Back to the Slide Revised 07/23/05